Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Gfra1

Uniprot No.

P97785

Research Area

Neuroscience

Alternative Names

Gfra1; Gdnfra; Trnr1; GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1; TGF-beta-related neurotrophic factor receptor 1

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

25-430aa

Target Protein Sequence

DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLTSGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRAVPFISDVFQQVEHISKGNNCLDAAKACNLDDTCKKYRSAYITPCTTSMSNEVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDVACTERRRQTIVPVCSYEERERPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSNCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEDCLKFLNFFKDNTCLKNAIQAFGNGSDVTMWQPAPPVQTTTATTTTAFRIKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGSTHLCLSDNDYGKDGLAGASS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

47.6

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer，50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.

Gene References into Functions

We further find that overexpression of NANOS2 results in the repression of GFRA1 and PLZF in gonocytes, leading to a delay in GST. On the other hand, loss of NANOS2 results in the up-regulation of GFRA1 and PLZF, indicating a precocious entry of GST. PMID: 29339164
Compromised Survival of Cerebellar Molecular Layer Interneurons Lacking GDNF Receptors GFRalpha1 or RET Impairs Normal Cerebellar Motor Learning. PMID: 28591570
GFRA1 regulates AMPK-dependent autophagy by promoting SRC phosphorylation independent of proto-oncogene RET kinase PMID: 27754745
the neural cell adhesion molecule NCAM is co-expressed and directly interacts with GFRalpha1 in embryonic Purkinje cells. PMID: 28076782
LncRNA033862 is an antisense transcript of the GDNF receptor alpha1 (Gfra1) that lacks protein coding potential and regulates Gfra1 expression levels by interacting with Gfra1 chromatin. PMID: 26962690
These findings collectively demonstrate that GFRalpha1 released by nerves enhances perineural invasion through GDNF-RET signaling and that GFRalpha1 expression by cancer cells enhances but is not required for it. PMID: 24778213
Gfra1 expression in the urinary tract strictly depends on cell-type and specific stage in kidney development. PMID: 23542432
our data demonstrate that RET/GFRalpha signals are dispensable for thymic T cell development in vivo PMID: 23300832
GFRalpha1 is critical for the development and function of the main olfactory system, contributing to the development and allocation of all major classes of neurons and glial cells. PMID: 23197722
Donor-derived GFRalpha1-positive cells formed several aggregates of A(al(aligned)) /morula-like structures in a single spermatogenic cell patch before and on day 14 post-transplant, indicating involvement in the formation of a stable spermatogenic colony. PMID: 22745058
GFRA1 expression is regulated by GDNF and in cultures of isolated seminiferous tubules, we found that GDNF expression and secretion by Sertoli cells is stage-dependent, being maximal in stages II-VI and decreasing thereafter. PMID: 22143971
Histone methylation and acetylation regulate Pou5f1 and Gfra1 in male germ cell. PMID: 20856864
Following ischemic injury, GFR alpha-1 is differentially up-regulated in GFR alpha-1 (+/+) and GFR alpha-1 (+/-) mice. PMID: 12697293
localization pattern of c-kit, Notch-1 and GFRalpha-1 in postnatal, immature and adult testes PMID: 15237212
GFR alpha-1, the receptor for GDNF is strongly expressed by a subset of type A spermatogonia. PMID: 15708562
The role of GFRalpha1 in the developing brain is to promote the differentiation and migration of cortical GABAergic neurons. PMID: 15748846
Data suggest that the combinatorial interactions of the spliced isoforms of GFRalpha, RET and NCAM may contribute to the pleiotropic biological responses. PMID: 15979200
Spermatogonial stem cells expresses GFRA1, but also the stem cell pool is heterogeneous with respect to the level of GFRA1 expression PMID: 16014811
Conditional ablation of GFRalpha1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung's disease phenotype. PMID: 17507417
Gfra1 is expressed dominantly in mouse spermatogonial stem cells; Gfra1 knockdown leads to their differentiation via the inactivation of RET tyrosine kinase, suggesting an essential role for Gfra1 in spermatogonial stem cell regulation. PMID: 17625109
The absence of Gfr alpha1 had no effect on either the appearance of brain-derived fatty acid binding protein (B-FABP) cells or the relative area of the ganglia occupied by B-FABP-immunoreactive cells. PMID: 18551627
The findings point to increased sensitivity of the DAergic system with age and neurotoxic exposure as a result of a genetic reduction of GFR alpha1. PMID: 18822276
The present study clearly establishes that genetic reductions in GFRalpha-1 can contribute to the degenerative changes observed in this system during the aging process. PMID: 18973577
From the data of this study suggested that GFR1 signaling guides the development of a subset of parvalbumin-expressing GABAergic interneurons populating discrete regions of the cerebral cortex. PMID: 19710321

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus, trans-Golgi network. Endosome. Endosome, multivesicular body.

Protein Families

GDNFR family

Tissue Specificity

Expressed in the brain, in hippocampal neurons (at protein level). Isoform 1 and isoform 2 are expressed in heart, brain, lung, liver, kidney and testis.

Database Links

KEGG: mmu:14585

STRING: 10090.ENSMUSP00000026076

UniGene: Mm.88367

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Code	CSB-MP009379MO
MSDS	MSDS
Size	$660
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse GDNF family receptor alpha-1 (Gfra1)

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