Recombinant Mouse Glucose-6-phosphate isomerase (Gpi)

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Code CSB-YP009717MO
MSDS
Size $306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
Gpi
Uniprot No.
Research Area
Others
Alternative Names
Gpi; Gpi1Glucose-6-phosphate isomerase; GPI; EC 5.3.1.9; Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI
Species
Mus musculus (Mouse)
Source
Yeast
Expression Region
2-558aa
Target Protein Sequence
AALTRNPQFQKLLEWHRANSANLKLRELFEADPERFNNFSLNLNTNHGHILVDYSKNLVNKEVMQMLVELAKSRGVEAARDNMFSGSKINYTENRAVLHVALRNRSNTPIKVDGKDVMPEVNRVLDKMKSFCQRVRSGDWKGYTGKSITDIINIGIGGSDLGPLMVTEALKPYSKGGPRVWFVSNIDGTHIAKTLASLSPETSLFIIASKTFTTQETITNAETAKEWFLEAAKDPSAVAKHFVALSTNTAKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDHFEQLLSGAHWMDQHFLKTPLEKNAPVLLALLGIWYINCYGCETHALLPYDQYMHRFAAYFQQGDMESNGKYITKSGARVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMKGKLPEEARKELQAAGKSPEDLEKLLPHKVFEGNRPTNSIVFTKLTPFILGALIAMYEHKIFVQGIMWDINSFDQWGVELGKQLAKKIEPELEGSSAVTSHDSSTNGLISFIKQQRDTKLE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
64.6kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Advance your metabolic research with our Recombinant Mouse Gpi, also known as Glucose-6-phosphate isomerase. This protein, recombinantly expressed in yeast, is a vital component in studies of carbohydrate metabolism and energy production pathways.

Our Gpi is a full-length mature protein (2-558aa), offering a comprehensive view of its function and structure. It is expressed with a N-terminal 6xHis-tag, ensuring efficient purification and reliable stability. With a purity greater than 90% as determined by SDS-PAGE, our Recombinant Mouse Gpi meets the rigorous requirements of precise scientific research. Opt for either the liquid form for immediate use or the lyophilized powder for long-term storage, suiting your research needs.

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Target Background

Function
In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.
Gene References into Functions
  1. PGI is a moonlighting protein that functions as a cytosolic enzyme involved in glycolysis and gluconeogenesis, and as cytokine through binding to its cell surface receptor. PMID: 8674049
  2. The mGPI-transgenic mice presented GPI more efficiently and showed a dramatic increase in negative selection and an inhibition of arthritis. PMID: 23840022
  3. Data show that silencing of AMF/PGI induced MET and suppressed the tumor growth and pulmonary metastasis of LM8 cells in nude mice. PMID: 20978190
  4. induces hyperpermeability of endothelial and mesothelial cells leading to accumulation of ascites fluid (tumor autocrine motility factor) PMID: 12054583
  5. acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils PMID: 12888574
  6. Gpi1 immunoreactivity was found in ganglion, amacrine, horizontal and bipolar cells, but not in rods, cones, pigment epithelium and Muller cells. PMID: 15182299
  7. High-resolution crystal structures of the enzyme from mouse have been determined in native form and in complex with the inhibitor erythrose 4-phosphate, and with the substrate glucose 6-phosphate. PMID: 15342241
  8. crystal structure of Autocrine Motility Factor PMID: 15502335
  9. AMF expression significantly contributes to the aggressive phenotype of pancreatic cancer PMID: 15570012
  10. Results report the first high-resolution crystal structure of the inhibitor-free form and the eight types of inhibitor complexes of mouse autocrine motility factor. PMID: 16375918
  11. the receptor molecule for AMF/NLK/MF in leukemic differentiation is not gp78 PMID: 17071500

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Subcellular Location
Cytoplasm. Secreted.
Protein Families
GPI family
Database Links

KEGG: mmu:14751

STRING: 10090.ENSMUSP00000049355

UniGene: Mm.589

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