Mouse Glucose-6-phosphate isomerase(GPI) ELISA kit

Instructions
Code CSB-EL009717MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name glucose phosphate isomerase
Alternative Names Gpi ELISA Kit; Gpi1Glucose-6-phosphate isomerase ELISA Kit; GPI ELISA Kit; EC 5.3.1.9 ELISA Kit; Autocrine motility factor ELISA Kit; AMF ELISA Kit; Neuroleukin ELISA Kit; NLK ELISA Kit; Phosphoglucose isomerase ELISA Kit; PGI ELISA Kit; Phosphohexose isomerase ELISA Kit; PHI ELISA Kit
Abbreviation GPI
Uniprot No. P06745
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 95    
Range % 90-99    
1:2 Average % 94    
Range % 87-98    
1:4 Average % 100    
Range % 94-105    
1:8 Average % 97    
Range % 91-102    
Recovery
The recovery of mouse GPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 97 89-102    
EDTA plasma (n=4) 102 95-107    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
50 2.538 2.422 2.480 2.308    
25 1.736 1.684 1.710 1.538    
12.5 1.197 1.143 1.170 0.998    
6.25 0.689 0.681 0.685 0.513    
3.12 0.414 0.389 0.402 0.230    
1.56 0.295 0.285 0.290 0.118    
0.78 0.223 0.226 0.225 0.053    
0 0.174 0.170 0.172      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
Gene References into Functions
  1. PGI is a moonlighting protein that functions as a cytosolic enzyme involved in glycolysis and gluconeogenesis, and as cytokine through binding to its cell surface receptor. PMID: 8674049
  2. The mGPI-transgenic mice presented GPI more efficiently and showed a dramatic increase in negative selection and an inhibition of arthritis. PMID: 23840022
  3. Data show that silencing of AMF/PGI induced MET and suppressed the tumor growth and pulmonary metastasis of LM8 cells in nude mice. PMID: 20978190
  4. induces hyperpermeability of endothelial and mesothelial cells leading to accumulation of ascites fluid (tumor autocrine motility factor) PMID: 12054583
  5. acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils PMID: 12888574
  6. Gpi1 immunoreactivity was found in ganglion, amacrine, horizontal and bipolar cells, but not in rods, cones, pigment epithelium and Muller cells. PMID: 15182299
  7. High-resolution crystal structures of the enzyme from mouse have been determined in native form and in complex with the inhibitor erythrose 4-phosphate, and with the substrate glucose 6-phosphate. PMID: 15342241
  8. crystal structure of Autocrine Motility Factor PMID: 15502335
  9. AMF expression significantly contributes to the aggressive phenotype of pancreatic cancer PMID: 15570012
  10. Results report the first high-resolution crystal structure of the inhibitor-free form and the eight types of inhibitor complexes of mouse autocrine motility factor. PMID: 16375918
  11. the receptor molecule for AMF/NLK/MF in leukemic differentiation is not gp78 PMID: 17071500

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Subcellular Location Cytoplasm, Secreted
Protein Families GPI family
Database Links

KEGG: mmu:14751

STRING: 10090.ENSMUSP00000049355

UniGene: Mm.589

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