Recombinant Mouse Heat shock protein 75 kDa, mitochondrial (Trap1)

Code CSB-EP863423MO
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP863423MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863423MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP863423MO
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Trap1
Uniprot No.
Alternative Names
Trap1; Hsp75; Heat shock protein 75 kDa; mitochondrial; HSP 75; TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1
Species
Mus musculus (Mouse)
Expression Region
61-706
Target Protein Sequence
STQAAEDKEE ESLHSIISNT EAVRGSVSKH EFQAETKKLL DIVARSLYSE KEVFIRELIS NASDALEKLR HKLVCEGQVL PEMEIHLQTD AKKGTITIQD TGIGMTQEEL VSNLGTIARS GSKAFLEALQ NQAETSSKII GQFGVGFYSA FMVADKVEVY SRSAAPESPG YQWLSDGSGV FEIAEASGVR PGTKIIIHLK SDCKDFASES RVQDVVTKYS NFVSFPLYLN GKRINTLQAI WMMDPKDISE FQHEEFYRYI AQAYDKPRFT LHYKTDAPLN IRSIFYVPEM KPSMFDVSRE LGSSVALYSR KVLIQTKAAD ILPKWLRFIR GVVDSEDIPL NLSRELLQES ALIRKLRDVL QQRLIKFFID QSKKDAEKYA KFFEDYGLFM REGIVTTAEQ DIKEDIAKLL RYESSALPAG QLTSLPDYAS RMQAGTRNIY YLCAPNRHLA EHSPYYEAMK QKHTEVLFCY EQFDELTLLH LREFDKKKLI SVETDIVVDH YKEEKFEDTS PADERLSEKE TEDLMAWMRN ALGSRVTNVK VTFRLDTHPA MVTVLEMGAA RHFLRMQQLA KTQEERAQLL QPTLEINPRH TLIKKLCQLR ESEPELAQLL VDQIYENAMI AAGLVDDPRA MVGRLNDLLV KVLEKH
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.
Gene References into Functions
  1. show that TRAP1 could protect the mitochondrial structure in renal tubular epithelial cells; maintain the levels of mitochondrial membrane potential, ATP, and mitochondrial DNA copy number PMID: 28710113
  2. TRAP1 increases cell proliferation, reduces apoptosis, and promotes cell invasion without changes in mitochondrial bioenergetics. Therefore, TRAP1 is a driver of prostate cancer in vivo and an "actionable" therapeutic target. PMID: 27754870
  3. Data show that the overexpression of heat shock protein 75 (Hsp75) decreased neural stem cells (NSCs) apoptosis and preserved mitochondrial membrane potential. PMID: 26500047
  4. TRAP-1-/- mice are viable and show reduced incidence of age-associated pathologies. Loss of TRAP-1 upregulates oxidative phosphorylation and glycolysis transcriptomes. PMID: 25088416
  5. overexpression of TRAP1 leads to mitochondrial aberrations, including an increase in basal ROS levels and decrease in mitochondrial biogenesis, together with a decrease in peroxisome proliferator-activated receptor gamma coactivator-1alpha mRNA levels PMID: 24286320
  6. Early mesangial nephritis initiates a cascade of inflammatory signals that lead to up-regulation of Trap1 and a consequent down-regulation of renal DNaseI by transcriptional interference. PMID: 23273922
  7. TRAP1 regulates a metabolic switch between oxidative phosphorylation and aerobic glycolysis in immortalized mouse fibroblasts. PMID: 23564345
  8. These data identify TRAP-1 as a novel mitochondrial survival factor differentially expressed in localized and metastatic prostate cancer compared with normal prostate. PMID: 19948822

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Subcellular Location
Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix.
Protein Families
Heat shock protein 90 family
Database Links
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