Recombinant Mouse Heat-stable enterotoxin receptor(Gucy2c),partial

In Stock
Code CSB-MP664972MO
Size US$1848Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names Gucy2c
Uniprot No. Q3UWA6
Research Area Microbiology
Alternative Names Gucy2c; Guc2c; Heat-stable enterotoxin receptor; STA receptor; EC 4.6.1.2; Guanylyl cyclase C; GC-C; Intestinal guanylate cyclase
Species Mus musculus (Mouse)
Source Mammalian cell
Expression Region 20-433aa
Target Protein Sequence VFWASQVRQNCRNGSYEISVLMMDNSAYKEPMQNLREAVEEGLDIVRKRLREADLNVTVNATFIYSDGLIHKSGDCRSSTCEGLDLLREITRDHKMGCALMGPSCTYSTFQMYLDTELNYPMISAGSYGLSCDYKETLTRILPPARKLMYFLVDFWKVNNASFKPFSWNSSYVYKNGSEPEDCFWYLNALEAGVSYFSEVLNFKDVLRRSEQFQEILTGHNRKSNVIVMCGTPESFYDVKGDLQVAEDTVVILVDLFSNHYFEENTTAPEYMDNVLVLTLPSEQSTSNTSVAERFSSGRSDFSLAYLEGTLLFGHMLQTFLENGENVTGPKFARAFRNLTFQGFAGPVTLDDSGDIDNIMSLLYVSLDTRKYKVLMKYDTHKNKTIPVAENPNFIWKNHKLPNDVPGLGPQILM
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 52.1 kDa
Protein Length Extracellular Domain
Tag Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin (By similarity).
Gene References into Functions
  1. Relative to controls, guanylate cyclase C knockout mice develop severe systemic illness following oral Salmonella infection, characterized by disrupted intestinal mucus layer, elevated cytokines and organ bacterial load, and reduced animal survival. PMID: 29367634
  2. Loss of Gucy2c expression is associated with Acute Radiation-Induced GI Syndrome. PMID: 28916678
  3. Findings show how caloric suppression of the guanylin-GUCY2C signaling axis links obesity to negation of a universal tumor suppressor pathway in colorectal cancer. PMID: 26773096
  4. The GC-C signaling pathway blunts colonic mucosal inflammation that is initiated by systemic cytokine burst or loss of mucosal immune cell immunosuppression. PMID: 24244444
  5. GC-C signaling is an essential component of host defense during murine enteric infection by reducing bacterial load and preventing systemic dissemination of attaching/effacing-lesion forming bacterial pathogens such as C. rodentium. PMID: 24004613
  6. guanylate cyclase-C PMID: 23739979
  7. GUCY2C opposes systemic genotoxic tumorigenesis by regulating AKT-dependent intestinal barrier integrity PMID: 22384056
  8. The role for GUCY2C-directed CD8(+) T cells targeting specific epitopes in antitumor efficacy. PMID: 22057677
  9. Data show that silencing of GUCY2C in mice disrupts satiation, resulting in hyperphagia and subsequent obesity and metabolic syndrome. PMID: 21865642
  10. a novel role for GC-C signaling in facilitating mucosal wounding and inflammation, and further suggest that this may be mediated, in part, through control of RELMbeta production PMID: 21555532
  11. midbrain dopamine neurons express GC-C; GC-C activation potentiates excitatory response mediated by glutamate and acetylcholine receptors via activity of PKG; GC-C knockout mice exhibit hyperactivity and attention deficit PMID: 21835979
  12. GCC signaling plays a protective role in the integrity of the intestinal mucosal barrier by regulating myosin light chain kinase activation and tight junction disassembly. PMID: 21305056
  13. There is a previously unappreciated interaction between sex and GCC signaling restricting crypt cell proliferation. PMID: 20443837
  14. GUCY2C is a tumor suppressor that controls proliferation and metabolism of intestinal epithelial cells by inactivating AKT signaling PMID: 19737566
  15. transmembrane guanylyl cyclase C is a key mediator of acid-stimulated duodenal bicarbonate secretion PMID: 12881226
  16. guanylin, uroguanylin, and E coli enterotoxin act on the mouse kidney, in part, through a cGMP-dependent, GC-C-independent mechanism, causing significant natriuresis by renal tubular processes PMID: 14675035
  17. two signaling pathways for guanylin peptides in principal cells of mouse cortical kidney collecting duct is not mediated by guanylate cyclase C PMID: 16105031
  18. GCC suppresses intestinal tumor initiation and growth by maintaining genomic integrity and restricting proliferation. PMID: 17681179
  19. GC-C coordinates component processes maintaining homeostasis of the crypt progenitor compartment. PMID: 17974601
  20. These findings demonstrate increased radiosensitivity of intestinal epithelial cells in guanylate cyclase C (GCC) knockout mice, and establish a role for GCC in preventing apoptosis. PMID: 19221018
  21. the role of the linker region in receptor guanylyl cyclases PMID: 19648115

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Subcellular Location Cell membrane, Single-pass type I membrane protein, Endoplasmic reticulum membrane, Single-pass type I membrane protein
Protein Families Adenylyl cyclase class-4/guanylyl cyclase family
Database Links

KEGG: mmu:14917

STRING: 10090.ENSMUSP00000032338

UniGene: Mm.222729

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