Recombinant Mouse Heparin cofactor 2 (Serpind1)

Code CSB-YP021080MO
MSDS
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Source Yeast
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Code CSB-EP021080MO
MSDS
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Source E.coli
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Code CSB-EP021080MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021080MO
MSDS
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Source Baculovirus
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Code CSB-MP021080MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Serpind1
Uniprot No.
Alternative Names
Serpind1; Hcf2; Hcii; Heparin cofactor 2; Heparin cofactor II; HC-II; Protease inhibitor leuserpin-2; Serpin D1
Species
Mus musculus (Mouse)
Expression Region
24-478
Target Protein Sequence
EQLTNEN LTTSFLPANF HKENTVTNDW IPEGEEDEDY LDLEKLLGED DDYIYIIDAV SPTDSESSAG NILQLFQGKS RIQRLNILNA KFAFNLYRVL KDQATTSDNL FIAPVGISTA MGMISLGLRG ETHEEVHSVL HFRDFVNASS KYEVTTIHNL FRKLTHRLFR RNFGYTLRSV NGLYIQKQFP IREDFKAAMR EFYFAEAQEA NFPDPAFISK ANNHILKLTK GLIKEALENI DPATQMLILN CIYFKGTWVN KFPVEMTHNH NFRLNEREVV KVSMMQTKGN FLAANDQELD CDILQLEYVG GISMLIVVPR KLSGMKTLEA QLTPQVVERW QKSMTNRTRE VLLPKFKLEK NYNLVEVLKS MGITKLFNKN GNMSGISDQR IAIDLFKHQS TITVNEEGTQ AAAVTTVGFM PLSTQVRFTV DRPFLFLVYE HRTSCLLFMG KVTNPAKS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
Gene References into Functions
  1. Loss of Heparin Cofactor is associated with insulin resistance . PMID: 28502917
  2. In this study, we demonstrate a previously unknown role for HCII in host defense PMID: 23656734
  3. HCII potentiates the activation of vascular endothelial cells and the promotion of angiogenesis in response to hindlimb ischemia via an AMPK-eNOS signaling pathway. PMID: 22904320
  4. HCII mediates the antithrombotic effect of porcine skin dermatan sulfate after injury to the carotid arterial endothelium in mice. PMID: 15315969
  5. In HCII(+/-) mice, prominent intimal hyperplasia with increased cellular proliferation was observed after tube cuff and wire vascular injury. PMID: 17549254
  6. HCII deficiency promotes atherogenesis and neointima formation PMID: 17878401
  7. These results support the hypothesis that HCII interacts with dermatan sulfate in the vessel wall after disruption of the endothelium and that this interaction regulates thrombus formation in vivo. PMID: 18281504

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Protein Families
Serpin family
Tissue Specificity
Expressed predominantly in liver.
Database Links
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