Recombinant Mouse Insulin-like growth factor-binding protein 1 (Igfbp1) is produced in an E. coli expression system and contains the full-length mature protein sequence from amino acids 26 to 272. The protein includes an N-terminal 6xHis-SUMO tag that makes purification and detection more straightforward. SDS-PAGE analysis shows the product achieves greater than 90% purity, which appears suitable for demanding research applications.
Insulin-like growth factor-binding protein 1 (IGFBP1) seems to play an important role in controlling insulin-like growth factor (IGF) activity through high-affinity binding interactions. The protein likely influences growth and metabolic processes by regulating how much IGF remains available to cells. Research into developmental biology and endocrine function has focused considerable attention on this protein.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Protein-Protein Interaction Studies with IGF System Components
Researchers can examine binding interactions between this recombinant mouse Igfbp1 and insulin-like growth factors (IGF-I and IGF-II) along with other IGF system components through in vitro binding assays. The N-terminal His-SUMO tag makes purification possible and allows immobilization for surface plasmon resonance or bio-layer interferometry experiments that measure binding kinetics and affinities. Scientists may identify new protein partners or confirm existing interactions in the IGF signaling pathway using pull-down assays with the His tag. The >90% purity level supports quantitative biochemical analysis of these interactions.
2. Antibody Development and Validation
The purified recombinant mouse Igfbp1 works well as an antigen for creating mouse IGFBP1-specific antibodies in research settings. High purity (>90%) suggests minimal cross-reactivity problems during immunization protocols for polyclonal or monoclonal antibody production. The protein can serve as both a positive control and standard in antibody validation experiments like Western blotting, ELISA, and immunoprecipitation assays. Detection and quantification during antibody screening becomes easier thanks to the His-SUMO tag.
3. Comparative Species Analysis and Evolutionary Studies
This mouse Igfbp1 protein may prove valuable in comparative biochemical studies when examined alongside IGFBP1 versions from other species to explore how binding properties and structural features have been conserved through evolution. Cross-species binding assays could reveal differences in IGF binding specificity and affinity between mouse and human IGFBP1 variants. The recombinant protein allows controlled in vitro experiments that examine species-specific differences in IGF system regulation. Such work might provide insights into how growth factor regulation mechanisms have adapted across different mammalian species.
4. Biochemical Characterization and Stability Studies
Researchers can subject the recombinant mouse Igfbp1 to detailed biochemical characterization, including thermal stability analysis, pH sensitivity testing, and proteolytic susceptibility assays. The His tag helps with protein quantification and tracking during stability experiments under various buffer conditions and temperatures. Circular dichroism spectroscopy offers a way to analyze secondary structure content and monitor conformational changes under different experimental conditions. These studies may provide essential biochemical data needed for optimizing storage conditions and experimental protocols for future research work.
