Mouse Insulin-like growth factor binding protein 1,IGFBP-1 ELISA Kit

Code CSB-E04587m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P47876
Lead Time 3-5 working days
Abbreviation IGFBP1
Target Name insulin-like growth factor binding protein 1
Alias AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1, IGF-binding protein 1|alpha-pregnancy-associated endometrial globulin|amniotic fluid binding protein|binding protein-25|binding protein-26|binding protein-28|gr
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 89
Range % 85-94
1:200 Average % 102
Range % 96-107
1:400 Average % 92
Range % 87-98
1:800 Average % 94
Range % 89-98
Recovery
The recovery of mouse IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 98 93-102
EDTA plasma (n=4) 103 92-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.179 2.234 2.207 2.081
10 1.816 1.943 1.880 1.754
5 1.337 1.345 1.341 1.215
2.5 0.839 0.846 0.843 0.717
1.25 0.405 0.412 0.409 0.283
0.625 0.278 0.265 0.272 0.146
0.312 0.182 0.189 0.186 0.060
0 0.125 0.126 0.126  
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Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration (By similarity).
Subcellular Location Secreted
Database Links

KEGG: mmu:16006

STRING: 10090.ENSMUSP00000020704

UniGene: Mm.21300

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