Product Details

Target Name

insulin-like growth factor binding protein 1

Alternative Names

Igfbp1 ELISA Kit; Igfbp-1 ELISA Kit; Insulin-like growth factor-binding protein 1 ELISA Kit; IBP-1 ELISA Kit; IGF-binding protein 1 ELISA Kit; IGFBP-1 ELISA Kit

Abbreviation

IGFBP1

Uniprot No.

P47876

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	89
	Range %	85-94
1:200	Average %	102
	Range %	96-107
1:400	Average %	92
	Range %	87-98
1:800	Average %	94
	Range %	89-98

Recovery

The recovery of mouse IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-102
EDTA plasma (n=4)	103	92-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.179	2.234	2.207	2.081
10	1.816	1.943	1.880	1.754
5	1.337	1.345	1.341	1.215
2.5	0.839	0.846	0.843	0.717
1.25	0.405	0.412	0.409	0.283
0.625	0.278	0.265	0.272	0.146
0.312	0.182	0.189	0.186	0.060
0	0.125	0.126	0.126

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse IGFBP1 ELISA Kit was designed for the quantitative measurement of Mouse IGFBP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Related Products

Target Background

Function
(From Uniprot)

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

Gene References into Functions

the hepatokine IGFBP1 is a critical liver-bone hormonal relay that promotes osteoclastogenesis and bone resorption as well as an essential mediator of FGF21-induced bone loss PMID: 26456333
Data suggest expression of Igfbp1 in liver is regulated by dietary factors; carnosine supplementation down-regulates expression of Igfbp1 in liver of obese/diabetic mice; mechanism involves suppression of Hif1a (hypoxia inducible factor 1 alpha). PMID: 25869614
serum IGFBPI protein could provide an easily measured biomarker for early detection of alcohol-induced liver injury in humans PMID: 24152801
These results show that RTEF-1-stimulated IGFBP-1 expression may be central to the mechanism by which RTEF-1 attenuates blood glucose levels. PMID: 22843786
Global deletion of Igfbp1 in a c-Myc transgenic model did not accelerate the development of prostate cancer. Global Igfbp1 deletion did result in a significant increase in body weight and body fat mass. PMID: 21903863
IGFBP-1 functions as a critical hepatic survival factor in the liver by reducing the level of proapoptotic signals PMID: 12511596
IGFBP-1 may support liver regeneration at least in part via its effect on MAPK/ERK and C/EBP beta activities. These findings are the first demonstration of the involvement of IGFBP-1 in the regulation of in vivo mitogenic signaling pathways. PMID: 12556485
Induction of IFGBP1 expression by amino acid deprivation of HepG2 hepatoma cells involves both a transcriptional activation and an mRNA stabilization due to its 3'UTR. PMID: 15862298
high concentrations of circulating IGFBP-1 are sufficient to cause fetal growth restriction in an animal model PMID: 16293667
the IGFBP-1 gene is a primary target of peroxisome proliferator-activated receptors PMID: 17062566
IGFBP-1 may have an important regulatory role in vivo and in the pathogenesis of type 2 diabetes, in which loss of insulin release is an early pathogenetic event PMID: 17693389
Ahr regulates Igfbp-1 and Lpl expression in the liver. TCDD treatment did not increase the Igfbp-1 and Lpl mRNA levels in Ahr knockout mice. PMID: 18827440
the embryopathic effects are associated with knockdown of EGNL1 and the associated induction of Igfbp1 mRNA in the placenta, but not the embryo. PMID: 19683511

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Subcellular Location

Secreted.

Database Links

KEGG: mmu:16006

STRING: 10090.ENSMUSP00000020704

UniGene: Mm.21300

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Mouse Insulin-like growth factor binding protein 1,IGFBP-1 ELISA Kit

Product Details

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Target Background