Recombinant Mouse Integrin beta-3(Itgb3),partial

Code CSB-YP011885MO1
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Source Yeast
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Code CSB-EP011885MO1
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Source E.coli
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Code CSB-EP011885MO1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011885MO1
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Source Baculovirus
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Code CSB-MP011885MO1
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Itgb3
Uniprot No. O54890
Alternative Names Itgb3; Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD antigen CD61
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surfaces. Fibrinogen binding enhances SELP expression in activated platelets. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling. ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling. ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition. ITGAV:ITGB3 act as a receptor for CD40LG.
Gene References into Functions
  1. Study results utilizing knockout mice suggest smooth muscle cells (SMCs) contribution to atherogenesis is regulated by integrin beta-3-mediated pathways in both SMCs and bone marrow-derived cells. PMID: 29802249
  2. inflammation induces expression of alphaVbeta3 Integrin and other proteins, astrocyte reactivity, and Thy-1 responsiveness. Importantly, ectopic control of beta3 Integrin levels modulates these responses regardless of inflammation. PMID: 28962574
  3. The integrin Beta3 Pro33 mutation disrupts the localization of 5-HT transporters to specific compartments within the synapse. PMID: 29038237
  4. our finding that CD61 is conservative in defining HECs both in vitro for hPSC differentiation and in vivo for mouse embryo provides valuable information on how to define and access the bipotent HECs. PMID: 27746115
  5. The authors showed the synergistic negative regulation of the pro-inflammatory cytokine interferon-gamma (IFNgamma) and beta3 integrin signaling in murine hematopoietic stem cells function by a novel definitive phenotyping of hematopoietic stem cells. PMID: 28673932
  6. This study provided evidence in platelets at both static and flow conditions that the calpain cleavage-related sequences of integrin beta3, i.e. T755NITYRGT762, R760GT762, and N756ITY759 participate in bidirectional, outside-in, and inside-out signaling, respectively and the association of c-Src or kindlin with beta3 integrin may regulate these processes. PMID: 27851790
  7. MiR-30a-5p suppresses tumor metastasis of human colorectal cancer by targeting ITGB3. PMID: 27576787
  8. suppression of beta3-integrin in mice leads to the activation of a neuropilin-1 (NRP1)-dependent cell migration pathway in endothelial cells via a mechanism that depends on NRP1's mobilisation away from mature focal adhesions following VEGF-stimulation PMID: 26159543
  9. Deletion of Itgb3 causes increased B-cell responses to TLR stimulation in vitro. PMID: 26965188
  10. integrin beta3-mediated signaling in smooth muscle cells links elastin deficiency and pathological stenosis, and inhibiting this pathway is an attractive therapeutic strategy for supravalvular aortic stenosis. PMID: 26858344
  11. The clathrin-mediated endocytic machinery combines with Dab2 to facilitate the endocytosis of RGD-integrin-beta3 clusters. PMID: 26507506
  12. studies identify alphavbeta3 as the primary integrin heterodimer mediating shear stress-induced proinflammatory responses and as a key contributor to early atherogenic inflammation PMID: 26212910
  13. Integrin beta 3 causes arteriovenous graft occlusion. PMID: 25614287
  14. Taken together, our results reveal that TD-26 is a novel antithrombotic compound exhibiting both integrin alphaIIbbeta3 inhibition and PI3K signaling blockage, with a low bleeding risk. PMID: 26278818
  15. Conformational activation of talin by PREL-1 triggers integrin-mediated cell adhesion. PMID: 25520155
  16. Conclude that tumour, rather than stromal, beta3 expression is essential and is required early for efficient spontaneous breast cancer metastasis to bone and soft tissues. PMID: 25430721
  17. in the presence of both Itgb3 and Slc6a4 heterozygozity, 5-HT uptake was similar to wild-type levels, revealing a significant Slc6a4 by Itgb3 genetic interaction PMID: 24083985
  18. Integrin beta3 mediates cerebrovascular remodelling during hypertension via Src/ClC-3 signalling pathway. PMID: 24611720
  19. Downregulation of TGF-beta1 and integrin beta3 impairs lymph node metastasis in a mouse model of non-small-cell lung cancer. PMID: 24884715
  20. We used MRI to image three genetic mouse models with single mutations implicated in autism: Neuroligin-3 R451C knock-in, Methyl-CpG binding protein-2 (MECP2) 308-truncation and integrin beta3 homozygous knockout. PMID: 24151012
  21. alphavbeta3-deficient mice displayed normal development of the virgin gland with no effect on luminal progenitors PMID: 25117682
  22. beta3-integrins have a role in RGD peptide-induced relaxation of pulmonary arteries and airways PMID: 24500921
  23. Angptl3 could induce actin filament rearrangement, mainly in lamellipodia formation, and that this process was mediated by integrin alpha(V)beta-mediated FAK and PI3K phosphorylation and Rac1 activation. PMID: 24294595
  24. beta3 integrin plays crucial role in blastocyst implantation; its co-expression with osteopontin is a biological marker for good endometrial receptivity PMID: 23870190
  25. In vivo analysis revealed that Spry4 regulated integrin beta3 levels in murine embryos and yolk sacs. PMID: 23955631
  26. C560Rbeta3 caused platelet integrin alphaII b beta3 to bind fibrinogen continuously, leading to hemorrhagic death consistent with Glanzmann thrombasthenia rather than a thrombotic state PMID: 23551977
  27. The results suggested that CD61 signaling is required for the proper growth of the cervical loop and for the promotion of the proliferation of pre-ameloblastic cells through Lgr5. PMID: 23553959
  28. Er'zhi Tiangui Granule could regulate the sequential expressions of integrinbeta3 and its ligand osteopontin to improve the mouse endometrial receptivity during controlled ovarian hyperstimulation. PMID: 23086489
  29. These results provide evidence for a direct role of integrin beta3 in the adhesion, migration and metastasis processes of mouse melanoma cells. PMID: 22895695
  30. Data indicate that beta3 integrin is required for Pyk2 cell surface localization and fibronectin accumulation. PMID: 22984613
  31. The pigment on the labial surface of mouse incisors is composed of Fe and that both anemia and reduction of iron-transporting proteins may cause the loss of pigmentation in CD61(-/-) mice. PMID: 23064962
  32. Genetic deletion of ITGbeta3 did not alter long-term potentiation or long-term depression in hippocampal slices. PMID: 22748100
  33. integrin beta(3)-deficiency in tumor stroma leads to an elevated IFP and lowered ECV that correlates with a more fibrotic ECM, underlining the role of the collagen network for carcinoma physiology PMID: 22479530
  34. These results indicate that both endothelial and platelet beta3 integrins contribute to extracellular PDI binding at the vascular injury site. PMID: 22653978
  35. Glycoprotein IIIa/IIb-targeted microbubbles specifically bind to activated platelets in vitro, allowing real-time molecular imaging of acute arterial thrombosis in a mouse model. PMID: 22647975
  36. beta3-integrin is required for differentiation in OC-2 cells, derived from E13 embryonic immortomouse inner ears. PMID: 22424110
  37. Integrin beta3 or its heterodimer alphavbeta3 is not critical for neutrophil migration into lungs infected with S. pneumoniae. PMID: 22350844
  38. we showed that the integrin-beta3 (CD61) signaling pathway was required for sustaining active TGFbeta signaling and self-renewal of TICs. PMID: 21996747
  39. the direct association between the cytoplasmic tails (CTs) of beta(3) and VEGFR2, is reported. PMID: 22363548
  40. study demonstrates that the epidermal growth factor receptor (EGFR) cooperates with beta3 integrin to regulate p190RhoGAP activity in mammary gland epithelial cells PMID: 21937717
  41. Integrin-beta3 plays a fundamental role in muscle regeneration through a regulation of macrophage infiltration and polarization leading to suppressed TGF-beta1 production. PMID: 22210777
  42. beta3 integrin interacts directly with GluA2 AMPA receptor subunit and regulates AMPA receptor expression in hippocampal neurons. PMID: 22232691
  43. data suggest THY1 plays a role in cell adhesion via binding to integrin beta3 in ovaries; integrin beta3 colocalizes with THY1 in developing ovarian follicles PMID: 21228213
  44. The expression and regulation of beta3-integrin in skeletal muscles following chemical injury with cardiotoxin are reported. PMID: 21350117
  45. low dose aspirin alters the expression of endometrial LIF and integrin beta3 PMID: 21035850
  46. Integrin beta3 plays a vital role in preventing cardiomyocyte apoptosis under conditions of oxidative stress in vitro. PMID: 20393597
  47. controls proliferation and migration of vascular smooth muscle cells in response to vascular injury under hyperglycemic conditions PMID: 20184965
  48. beta3-integrins have cell-specific roles in complex biological processes PMID: 19933310
  49. These results show a function for the alphavbeta3 integrin in the regulation of protein permeability, selective for the heart capillaries. PMID: 19645751
  50. Data provide a proof for the concept that the function of TG2 is to stabilize accumulated integrin beta(3) concentration in the phagocytic cup. PMID: 19643138

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Subcellular Location Cell membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane. Cell junction, focal adhesion. Cell junction, synapse, postsynaptic cell membrane; Single-pass type I membrane protein. Cell junction, synapse.
Protein Families Integrin beta chain family
Database Links

KEGG: mmu:16416

STRING: 10090.ENSMUSP00000021028

UniGene: Mm.87150

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