Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Irf5

Uniprot No.

P56477

Research Area

Others

Alternative Names

Irf5Interferon regulatory factor 5; IRF-5

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-497aa

Target Protein Sequence

MNHSAPGIPPPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFYIPWRHATRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFQLFYDGPRDMPPQPYKIYEVCSNGPAPTESQPTDDYVLGEEEEEEEEELQRMLPGLSITEPALPGPPNAPYSLPKEDTKWPPALQPPVGLGPPVPDPNLLAPPSGNPAGFRQLLPEVLEPGPLASSQPPTEPLLPDLLISPHMLPLTDLEIKFQYRGRAPRTLTISNPQGCRLFYSQLEATQEQVELFGPVTLEQVRFPSPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCALAHGSCPNPIQREVKTKLFSLEQFLNELILFQKGQTNTPPPFEIFFCFGEEWPDVKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDHMVEQFKELHHLWQSQQQLQPMVQAPPVAGLDASQGPWPMHPVGMQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

72.0kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

In the production of recombinant Mouse Irf5 protein, the gene for Irf5 (E.coli) was cloned into a vector and expressed as Irf5 protein in E.coli. The plasmids with the copy of Irf5, or the expression vector, were often used to enhance gene expression. Every step of production was undergone with a strict QC system. N-terminal 6xHis-SUMO tag was used in the process. The purity is 90% determined by SDS-PAGE.

IRF5 is the member of the interferon-regulatory factor (IRF) family, has diverse activities, such as the activation of genes encoding type I interferon, inflammatory cytokines (including tumor necrosis factor, IL-6, IL-12 and IL-23) and tumor suppressors. IRF5 is constitutively expressed in lymphoid tissues such as spleen and peripheral blood lymphocytes. In the latter, the expression of IRF5 is mainly found in B cells and barely in T cells and natural killer cells. Higher level of IRF5 expression has been found in other immune cells such as monocytes and macrophages but relatively lower in dendritic cells. Irf5-deficient mice are resistant to lethal endotoxic shock. Researches implied that IRF5 expression in macrophages was reversibly induced by inflammatory stimuli and contributed to the plasticity of macrophage polarization. High expression of IRF5 was characteristic of M1 macrophages, in which it directly activated transcription of the genes encoding interleukin 12 subunit p40 (IL-12p40), IL-12p35 and IL-23p19 and repressed the gene encoding IL-10. Consequently, those macrophages set up the environment for a potent T helper type 1 (TH1)-TH17 response.

Target Background

Function

Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway.

Gene References into Functions

Scavenger receptor class A is regulated by pathogens and suppresses IRF5 nuclear translocation by direct interaction. Reduced abundance of nuclear IRF5 shifts macrophage polarization from M1 towards M2, which subsequently switches T-helper responses from type 1 to type 2. PMID: 28695899
Aged mice expressed higher IRF5 levels in the ischemic brains, suggesting that aging has a significant influence on stroke outcomes in mice, which is probably mediated by age-specific inflammatory responses. PMID: 28905935
this study shows a critical role for IRF5 in regulating allergic airway inflammation PMID: 27759022
IRF5 and IRF5 disease-risk variants increase glycolysis and human m1 macrophage polarization by regulating proximal signaling and Akt2 activation. PMID: 27545875
these results reveal a role for Lyn as a specific suppressor of the TLR-MyD88-IRF5 pathway and illustrate the importance of fine-tuning IRF5 activity for the maintenance of immune homeostasis PMID: 27521268
IRF5 siRNA reverses pancreatitis-induced activation of lung macrophages from M1 phenotype to M2 phenotype in severe acute pancreatitis associated with acute lung injury. PMID: 27895424
Our data show that miR-146b targets IRF5, resulting in the regulation of macrophage activation. Furthermore, miR-146b deficient mice developed intestinal inflammation with enhanced M1 macrophage polarization. PMID: 27825654
data suggest that IRF5 plays a causal role in inflammation, fibrosis and impaired vascular EC function in Tsk/+ mice PMID: 27050551
Collectively, these results indicate a key role for IRF-5 in modulating the host antiviral response in peripheral organs that controls bunyavirus neuroinvasion in mice. PMID: 26468541
studies extend prior ones suggesting that inhibiting IRF5 might be useful for chronic macrophage-induced inflammation and suggest that IRF5 blockade would ameliorate more acute forms of inflammation, including lung injury PMID: 26283380
IRF5 plays an important role in the development of disease in the MRL/lpr mouse model of lupus in the absence of the DOCK2 mutation and that IRF5 is required for the transition from mature B cells to plasma cells. PMID: 25076492
IRF5 expression in microglia is regulated by IRF8. IRF5 directly upregulates P2RX4 expression on microglia in peripheral nerve injury, and may play a role in neuropathic pain. PMID: 24818655
PAR1 suppression of IRF5 and IL-12/23 secretion by macrophages provides a novel mechanism by which the host suppresses the mucosal Th1 and Th17 response to H. pylori infection. PMID: 24866378
This study uncovers a new function for IRF5 in controlling the relative mass of different adipose tissue depots and thus insulin sensitivity in obesity. PMID: 25939064
IKKbeta is an IRF5 kinase that instigates inflammation PMID: 25326420
our results reveal a protective role for IRF5 in lupus-associated atherosclerosis that is mediated through the effects of IRF5 in both immune and nonimmune cells. PMID: 25595782
IRF5 has a critical role in shaping the early innate immune response towards West Nile virus in the draining lymph node, which impacts the spread of virus infection, optimal B cell immunity, and disease pathogenesis. PMID: 25031348
The IRF5 deficiency reduces IFN-alpha, IFN-beta and IL-6 production by Toll-like receptor 9 (TLR9)- and TLR7-stimulated DCs and reduces TLR7- and TLR9-induced IL-6 production by B cells PMID: 23291967
establish the species-invariant role of IRF5 in controlling the inflammatory macrophage phenotype both in vitro and in in vivo PMID: 24453413
These proteins were upregulated in Irf5(-/-) mice. PMID: 23912454
Reduction of IRF5 expression, a factor that regulates macrophage polarization, reduced expression of inflammatory M1 macrophage markers, supported resolution of inflammation, accelerated cutaneous and infarct healing PMID: 24361318
IRF5 levels in Tsk(-/+) hearts were similar to control. PMID: 23251680
Our results establish IRF-3, -5, and -7 as the key transcription factors responsible for mediating the type I IFN and IFN stimulated gene response in myeloid dendritic cells during WNV infection and suggest a novel signaling link between MAVS and IRF-5. PMID: 23300459
Our results show that IRF5 is a crucial driver of lupus development in mice. PMID: 21305501
Irf5-deficient monocytes are defective in their response to peritoneal lavage of pristane-injected mice; pristane-induced Ly6C(hi) antigen-producing monocyte recruitment to the peritoneal cavity is impaired in Irf5-deficient mice. PMID: 22933628
these results identify the IRF-5-Ikaros axis as a critical modulator of IgG2a/c class switching. PMID: 22535200
overexpression of IRF5 in blood cells of systemic lupus erythematosus(SLE) patients may contribute to disease pathogenesis. PMID: 22678902
uncovered a new, IFN-I-independent role of IRF5 in regulating chemokines involved in the homing of pDCs and certain lymphocyte subsets. PMID: 22422888
A spontaneous genomic duplication and frameshift mutation in the guanine exchange factor dedicator of cytokinesis 2 (Dock2) that has arisen in at least a subset of circulating Irf5(-/-) mice and inadvertently been bred to homozygosity. PMID: 22431588
the IRF5/Blimp-1 axis differentially regulates the expression of Nba2 lupus susceptibility genes, and they suggest an important role for the IRF5/Blimp-1/p202 axis in murine lupus susceptibility. PMID: 22116829
The authors show that the myxoma virus-induced innate immune response requires TLR9 and its adaptor MyD88, transcription factors IRF5 and IRF7, and the type I interferon positive-feedback loop mediated by IFNAR1. PMID: 21835795
Levels of Irf5 mRNA and protein were higher in females than in age-matched males. PMID: 20802013
Demonstrate a requirement for IRF5 in the development of SLE via its role in B cells. Antinuclear autoantibodies and Ig glomerular deposits, hallmarks of SLE, are absent in Irf5(-/-) mice challenged to develop SLE by pristane injection. PMID: 20479222
Data show the role of IRF-5 in the B-cell terminal differentiation. PMID: 20176957
IRF5 plays an essential role in lupus pathogenesis in murine models and this is mediated through pathways beyond that of type I IFN production PMID: 20007534
study identifies IRF-5 as a new, principal downstream regulator of the TLR-MyD88 signalling pathway and a potential target of therapeutic intervention to control harmful immune responses PMID: 15665823
we also provide evidence that this TLR synergy is mediated, at least in part, by activation of the transcription factor interferon regulatory factor 5 (IRF5). PMID: 17275788
IRF5 is critical for antiviral immunity and for the induction of apoptosis, but not cell cycle arrest in response to DNA damage, and acts as a tumor suppressor on a pathway that may be distinct from that for p53. PMID: 17360658
IRF5 plays a critical role in interferon (IFN)-alpha and IFN-beta production induced not only by RNA-containing immune complexes but also by conventional Toll-like receptor (TLR)7 and TLR9 ligands. PMID: 17513736
reveal a cell-type-specific function for IRF5 in the complex regulatory mechanism of death-receptor-induced apoptosis PMID: 18268344
reveal the cell type-specific importance of IRF-5 in MyD88-mediated antiviral pathways and the widespread role of IRF-5 in the regulation of inflammatory cytokines PMID: 18332133
Data show that IRF-5, which is activated via the MyD88 pathway, is subjected to TRAF6-mediated K63-linked ubiquitination, which is important for IRF-5 nuclear translocation and target gene regulation. PMID: 18824541
The functional characterization of the novel IRF-5 mutant in transformed B and T cell lines and in ATL and CLL patients may lead to a better understanding of the role of these transcriptional regulators in hematopoietic malignancies. PMID: 19430534

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

IRF family

Database Links

KEGG: mmu:27056

STRING: 10090.ENSMUSP00000004392

UniGene: Mm.6479

Code	CSB-EP011820MO
Abbreviation	Recombinant Mouse Irf5 protein
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Interferon regulatory factor 5 (Irf5)

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