Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Mouse Il1rap protein at 2 μg/mL can bind Anti-Il1RAP recombinant antibody (CSB-RA878844MA2HU). The EC50 is 2.279-3.955 ng/mL.

Target Names

Il1rap

Uniprot No.

Q61730

Alternative Names

Interleukin-1 receptor accessory protein; EC:3.2.2.6; IL-1 receptor accessory protein; IL-1RAcP; Interleukin-33 receptot beta chain; Il1rap

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

21-367aa

Target Protein Sequence

SERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKYNYSTAHSSGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSAMRFPVHKMYIEHGIHKITCPNVDGYFPSSVKPSVTWYKGCTEIVDFHNVLPEGMNLSFFIPLVSNNGNYTCVVTYPENGRLFHLTRTVTVKVVGSPKDALPPQIYSPNDRVVYEKEPGEELVIPCKVYFSFIMDSHNEVWWTIDGKKPDDVTVDITINESVSYSSTEDETRTQILSIKKVTPEDLRRNYVCHARNTKGEAEQAAKVKQKVIPPRYTVELACGFGAT

Mol. Weight

41.4 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

IL-1 receptor accessory protein forms an essential co-receptor complex with IL-1R1 to transduce interleukin-1 and interleukin-33 signaling, making it a critical node in inflammatory and innate immune responses. This mammalian-expressed construct spanning residues 21–367 captures the complete extracellular domain required for ligand recognition and receptor assembly, ensuring native post-translational modifications that preserve conformational epitopes. Functional ELISA validation demonstrates specific antibody binding with an EC50 of 2.279–3.955 ng/mL, confirming that the protein retains its native structure and supports applications including therapeutic antibody epitope mapping, blocking antibody screening, and ligand-receptor interaction studies by ELISA, SPR, or BLI. The combination of >95% purity and <1.0 EU/μg endotoxin meets the quality thresholds commonly required for competitive inhibition assays and serves as a validated positive control in binding experiments targeting IL-1 and IL-33 signaling pathways.

Target Background

Function

Coreceptor for IL1RL2 in the IL-36 signaling system. Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD. May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells.; Associates with secreted ligand-bound IL1R2 and increases the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Enhances the ability of secreted IL1R1 to inhibit IL-33 signaling.; Required for Src phosphorylation by IL1B. Required for IL1B-potentiated NMDA-induced calcium influx in neurons acting in cooperation with IL1R1 isoform 2 to mediate Akt kinase activation.

Gene References into Functions

These results thus reveal the decoding mechanism of splice-insert signaling codes for synaptic differentiation induced by trans-synaptic adhesion between PTPdelta and IL1RAPL1/IL-1RAcP. PMID: 25908590
We conclude that neuron-specific AcPb plays a critical role in host defenses and sleep homeostasis. PMID: 25449578
In experimental biliary atresia, miR-29a/29b1 are upregulated, and reporter assays confirmed that Igf1 and Il1RAP are down-regulated by miR-29. PMID: 22167021
The results of this study suggested that IL-1RAcP represents an interesting molecular link between immune systems and synapse formation in the brain. PMID: 22357843
IL-1RAcP is essential for physiological activities of peripheral IL-1. PMID: 11847485
Identification of essential regions in the cytoplasmic tail of interleukin-1 receptor accessory protein critical for interleukin-1 signaling PMID: 11880380
determination of interaction sites in vitro mutagenesis and molecular modeling PMID: 13679373
data suggest that domain III of IL-1RAcP may be involved in the formation or stabilization of the IL-1RI/IL-1 complex by binding to epitopes on domain III of the IL-1RI created following IL-1 binding to the IL-1RI PMID: 17669273
IL-33 and ST2 form a complex with IL-1R accessory protein (IL-1RAcP), a signaling receptor subunit that is also a member of the IL-1R complex. PMID: 17675517
IL-1RAcP is used by more than one alpha-chain of the IL-1 receptor family and thus may resemble a common beta-chain of that family PMID: 18003919
these observations establish AcP as co-receptor for IL-33 signaling via ST2 and suggest a novel role for sAcP in modulating the biological activity of IL-33 PMID: 18450470
an isoform of the IL-1 receptor accessory protein (termed AcPb) was identified that is expressed exclusively in the CNS. PMID: 19481478

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Subcellular Location

[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.

Protein Families

Interleukin-1 receptor family

Tissue Specificity

Detected in lung, brain, spleen, thymus and liver. Expressed in brain endothelial cells, astrocytes, microglia and neurons. Isoform 3 is predominantly expressed in brain; expressed in hippocampal neurons.

Database Links

KEGG: mmu:16180

UniGene: Mm.253424

Code	CSB-MP730751MO
Abbreviation	Recombinant Mouse Il1rap protein, partial (Active)
MSDS	MSDS Datasheet
Size	$9.9
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Mouse Il1rap protein at 2 μg/ml can bind Anti-Il1RAP recombinant antibody (CSB-RA878844MA2HU). The EC₅₀ is 2.279-3.955 ng/mL. Biological Activity Assay
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Recombinant Mouse Interleukin-1 receptor accessory protein (Il1rap), partial (Active)

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