Recombinant Mouse Keratin, type I cytoskeletal 17 (Krt17)

Code CSB-YP889547MO
MSDS
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Source Yeast
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Code CSB-EP889547MO
MSDS
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Source E.coli
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Code CSB-EP889547MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP889547MO
MSDS
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Source Baculovirus
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Code CSB-MP889547MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Krt17
Uniprot No.
Alternative Names
Krt17; Krt1-17Keratin; type I cytoskeletal 17; Cytokeratin-17; CK-17; Keratin-17; K17
Species
Mus musculus (Mouse)
Expression Region
1-433
Target Protein Sequence
MTTTIRQFTS SSSIKGSSGL GGGSSRTSCR LSGSLGAGSC RLGSASGLGS ALGSNSYSSC YSFGTGSGYG GNFGGVDGLL AGGEKATMQN LNDRLASYLD KVRALEEANT ELEVKIRDWY QKQAPGPARD YSAYYHTIED LKNKILVATV DNASILLQID NARLAADDFR TKFETEQALR MSVEADINGL RRVLDELTLA RADLEMQIEN LKEELAYLKK NHEEEMNALR GQVGGEINVE MDAAPGVDLS RILSEMRDQY EKMAEKNRKD AEDWFFSKTE ELNREVATNS ELVQSGKSEI SELRRTMQAL EIELQSQLSM KASLEGSLAE TENRYCVQLS QIQGLIGSVE EQLAQLRCEM EQQNQEYKIL LDVKTRLEQE IATYRRLLEG EDAHLTQYKP KEPVTTRQVR TIVEEVQDGK VISSREQVHQ TTR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors. May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.
Gene References into Functions
  1. Narrow-band ultraviolet B irradiation with doses of MED or higher could eliminate the imiquimod-induced psoriasis-like dermatitis and inhibit K17 expression via the ERK1/2 and STAT3 signaling pathways. PMID: 29349514
  2. TGF-beta1-induced CK17 enhances cancer stem cell-like properties rather than epithelial-mesenchymal transition in promoting cervical cancer metastasis via the ERK1/2-MZF1 signaling pathway. PMID: 28703907
  3. the data suggest that the loss of Krt17 may foster an overall protective environment for lesion-prone cervical tissue. PMID: 27065324
  4. High KR17 expression is associated with oral cancer. PMID: 27512993
  5. p53 operates as a direct Krt17 repressor that invites therapeutic targeting in radiodermatitis . PMID: 26747697
  6. genetic loss of caspase-7 promotes solar-simulated light-induced skin carcinogenesis by blocking caspase-7-mediated cleavage of keratin-17. PMID: 26271098
  7. Data indicate that keratin 17 (k17) regulates autoimmune regulator Aire to promote skin tumorigenesis. PMID: 26168014
  8. These findings functionally integrate K17, hnRNP K, and gene expression along with RSK and CXCR3 signaling in a keratinocyte-autonomous axis and provide a potential basis for their implication in tumorigenesis PMID: 25713416
  9. KRT17 is necessary for oncogenic transformation in Ewing sarcoma and accounts for much of the GLI1-mediated transformation function but via a mechanism independent of AKT signaling. PMID: 24043308
  10. Cytochrome b5 and cytokeratin 17 are biomarkers in bronchoalveolar fluid signifying onset of acute lung injury. PMID: 22792238
  11. IL-22 up-regulates K17 expression in keratinocytes in a dose-dependent manner through STAT3- and ERK1/2-dependent mechanisms. PMID: 22808266
  12. Type I keratin 17 protein is phosphorylated on serine 44 by p90 ribosomal protein S6 kinase 1 (RSK1) in a growth- and stress-dependent fashion PMID: 22006917
  13. Keratin 17 promotes epithelial proliferation and tumor growth by polarizing the immune response in skin. PMID: 20871598
  14. Keratin 17 null mice exhibit age- and strain-dependent alopecia PMID: 12050118
  15. Induction of K6 and K17 correlates with changes in epithelial cells at wound edge. Closure of embryonic wounds is delayed in K17 null embryos. Correlation between K6 and K17 expression and epithelial wound closure. Important for timeliness of process. PMID: 12557214
  16. Gene located immediately 5' upstream from mK17 is functional and encodes a type I keratin protein highly analogous to mK17. Expression is highly restricted and most prevalent in nail bed and matrix, mK17n (n stands for nail). (K17n) PMID: 15102087
  17. K6alpha, K6beta, and K17 have roles in normal development of the nail bed epithelium PMID: 15601842
  18. sonic hedgehog participates in K17 regulation PMID: 16055733
  19. These findings identify K17 and TNFalpha as two novel and interdependent regulators of hair cycling. PMID: 16702408
  20. keratin 17, an intermediate filament protein rapidly induced in wounded stratified epithelia, regulates cell growth through binding to the adaptor protein 14-3-3sigma PMID: 16710422

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Subcellular Location
Cytoplasm.
Protein Families
Intermediate filament family
Tissue Specificity
Expressed strongly in outer root sheath and medulla region of hair follicle and in the early differentiating epithelial cells (trichocytes) within the hair bulb region. Weak expression in the matrix cells of hair bulb. Also present in the sweat gland with
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