Recombinant Mouse LIM domain-containing protein ajuba (Ajuba)

Code CSB-YP856315MO
MSDS
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Source Yeast
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Code CSB-EP856315MO
MSDS
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Source E.coli
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Code CSB-EP856315MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856315MO
MSDS
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Source Baculovirus
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Code CSB-MP856315MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ajuba
Uniprot No.
Alternative Names
Ajuba; Jub; LIM domain-containing protein ajuba
Species
Mus musculus (Mouse)
Expression Region
1-547
Target Protein Sequence
MERLGEKASR LLEKLRLSDS GSAKFGRRKG EASRSGSDGT PGAGKGRLSG LGGPRKSGHR GANGGPGDEP LEPAREQGPL DAERNARGSF EAQRFEGSFP GGPPPTRALP LPLSSPPDFR LETTAPALSP RSSFASSSAS DASKPSSPRG SLLLDGAGAS GAGGSRPCSN RTSGISMGYD QRHGSPLPAG PCLFGLPLTT APAGYPGGAP SAYPELHAAL DRLCAHRSVG FGCQESRHSY PPALGSPGAL TGAVVGTAGP LERRGAQPGR HSVTGYGDCA AGARYQDELT ALLRLTVATG GREAGARGEP SGIEPSGLEE SPGPFVPEAS RSRIREPEAR EDYFGTCIKC NKGIYGQSNA CQALDSLYHT QCFVCCSCGR TLRCKAFYSV NGSVYCEEDY LFSGFQEAAE KCCVCGHLIL EKILQAMGKS YHPGCFRCIV CNKCLDGVPF TVDFSNQVYC VTDYHKNYAP KCAACGQPIL PSEGCEDIVR VISMDRDYHF ECYHCEDCRM QLSDEEGCCC FPLDGHLLCH GCHMQRLSAR QPSTNYI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1.
Gene References into Functions
  1. Ajuba recruits p300/CBP via its LIM domain and facilitates p300/CBP binding to PPARg. Moreover, Ajuba, PPARg, p300/CBP can cooperatively occupy the PPARg target promoters and concomitantly increases histone acetylation at these loci. PMID: 26113042
  2. Ajuba is a novel coactivator for liver X receptors and may play important role in lipid and glucose metabolism. PMID: 26389695
  3. Findings support the importance of adhesion molecules (VE-cadherin and CD31), survivin, and Ajuba in modulating the Hippo pathway, which regulates, in part, proliferation and survival in hemangioendotheliomas. PMID: 25266662
  4. PKD1-mediated phosphorylation of SNAI1 occurs in the nucleus and generates a nuclear, inactive DNA/SNAI1 complex that shows decreased interaction with its co-repressor Ajuba. PMID: 22276203
  5. This paper presents evidence indicating that the human and mouse Ajuba is a new cytosolic component of the IL-1 signaling pathway, influencing the assembly and activity of the aPKC/p62/TRAF6 multiprotein signaling complex. PMID: 15870274
  6. identification of the protein arginine methyltransferase 5 (PRMT5) as an effector recruited to SNAIL through an interaction with AJUBA that functions to repress the SNAIL target gene, E-cadherin PMID: 18347060

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Subcellular Location
Cytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, P-body.
Protein Families
Zyxin/ajuba family
Tissue Specificity
Expressed in skin, brain and genitourinary organs.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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