Recombinant Mouse Mannose-binding protein C (Mbl2)

In Stock
Code CSB-EP013540MO
Abbreviation Recombinant Mouse Mbl2 protein
MSDS
Size $256
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Target Names
Uniprot No.
Research Area
Immunology
Alternative Names
MBP-C;Mannan-binding protein;RA-reactive factor P28A subunit;RARF/P28A
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
19-244aa
Target Protein Sequence
ETLTEGVQNSCPVVTCSSPGLNGFPGKDGRDGAKGEKGEPGQGLRGLQGPPGKVGPTGPPGNPGLKGAVGPKGDRGDRAEFDTSEIDSEIAALRSELRALRNWVLFSLSEKVGKKYFVSSVKKMSLDRVKALCSEFQGSVATPRNAEENSAIQKVAKDIAYLGITDVRVEGSFEDLTGNRVRYTNWNDGEPNNTGDGEDCVVILGNGKWNDVPCSDSFLAICEFSD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
30.9
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages.
Gene References into Functions
  1. this study shows that MBL-C expression is induced in the gut in response to Candida albicans sensing and is required for intestinal homeostasis and host defense against Candida albicans PMID: 26442658
  2. Human and murine data together indicate that SP-A, SP-D and MBL are synthesized in early gestational tissues, and may contribute to regulation of immune response at the feto-maternal interface during pregnancy. PMID: 26603976
  3. These data show that modulation of MBL is involved in the protection against renal I/RI induced by dietary restriction, and suggest that the mechanisms of protection induced by dietary restriction and fasting may be different. PMID: 26367533
  4. demonstrate that apolipoprotein E (Apoe), mannose-binding lectin 2 (Mbl2), and parotid secretory protein (Psp) are present at significantly different quantities in depleted plasma of diabetic NZO mice compared to non-diabetic controls PMID: 23350727
  5. These observations point to a previously unappreciated role for MBL in regulating host resistance and cardiac inflammation during infection with a major human pathogen. PMID: 23139754
  6. MBL plays a key role in clearing influenza A virus and maintaining lung homeostasis. PMID: 21182784
  7. present in the decidua in abortion-prone mating combinations PMID: 19854517
  8. selectively expressed in villous epithelial cells of the small intestine PMID: 12471128
  9. role for MBL in clearance of dying cells PMID: 15749852
  10. exposure of MBL to Kupffer cells increased cell surface SR-A expression and phagocytosis of S. aureus and E. coli; and MBL bound to lipid A, LPS, and S. aureus PMID: 17015738
  11. MBL as an important molecule in the maintenance of the homeostatic balance in the skin PMID: 17363917
  12. both the presence of capsule and wild-type cell wall architecture preclude MBL binding to C. neoformans. PMID: 17646356
  13. The in vitro binding and activation of the human and mouse complement systems was analysed and the susceptibility to infection in complement-deficient mouse strains, was tested. PMID: 18501966
  14. These model studies have uncovered unexpected roles of MBL and evidence of its interaction with other molecules of the innate immune system. PMID: 19021581
  15. mMBL-C may protect host intestinal mucosa by directly binding to the bacteria. PMID: 19684159

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Subcellular Location
Secreted.
Database Links
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