Recombinant Mouse Metalloproteinase inhibitor 4 (Timp4)

Code CSB-YP867470MO
MSDS
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Source Yeast
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Code CSB-EP867470MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP867470MO
MSDS
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Source Baculovirus
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Code CSB-MP867470MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Timp4
Uniprot No.
Alternative Names
Timp4; Metalloproteinase inhibitor 4; Tissue inhibitor of metalloproteinases 4; TIMP-4
Species
Mus musculus (Mouse)
Expression Region
30-224
Target Protein Sequence
C SCAPAHPQQH FCHSALVIRA KISSEKVVPA SKDPADTQKL IRYEIKQIKM FKGFEKAKDI QYVYTPFDSS LCGVKLETNS HKQYLLTGQI LSDGKVFIHL CNYIEPWEDL SLVQRESLNH HYHQNCGCQI TTCYAVPCTI SAPNECLWTD WLLERKLYGY QAQHYVCMKH VDGICSWYRG HLHLRKEYVD IIQP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
Gene References into Functions
  1. there are 7 CpG islands in the TIMP4 promoter which get methylated during the progression of heart failure which leads to its epigenetic silencing. PMID: 27396717
  2. High fat diet-induced obesity downregulates adipocyte MMP3 expression to trigger adipogenesis, and adipocyte TIMP4 may modulate this process to regulate hyperplastic vs. hypertrophic adipose tissue expansion, fat distribution, and metabolic health in a sex- and depot-dependent manner. PMID: 27879248
  3. Our findings highlight a protective mechanism induced by PPARgamma in deoxycorticosterone acetate-salt treatment, establishing a novel mechanistic link between PPARgamma and TIMP-4. PMID: 26597823
  4. TIMP4 augments contractility and induces differentiation of embryonic stem cells into cardiac phenotype. PMID: 25745981
  5. TIMP-4 might act as a negative regulator of adipogenesis through NFkB cascade modulation. PMID: 25999146
  6. Replenishment of myocardial TIMP4 could serve as an effective therapy in post-I/R recovery for patients with reduced TIMP4. PMID: 24842912
  7. TIMP-4 protein was localized to corpus luteum and theca-intera cells in various periods throughout the pregnancy and postpartum day 1. PMID: 21141538
  8. Timp4(-/-) mice are more susceptible to MI but not to pressure overload, and TIMP4 functions in its capacity as a metalloproteinase inhibitor after myocardial infarction PMID: 20516072
  9. Identification of an initiator-like element essential for the expression of the tissue inhibitor of metalloproteinases-4 (Timp-4) gene PMID: 11988080
  10. molecular cloning and localization of the gene within intron 5 of the synapsin 2 gene PMID: 12151094
  11. This study suggests that tissue inhibitor of metalloproteinase-4 may restrict mouse blastocyst outgrowth and embryo implantation by inhibiting the activities of matrix metalloproteinases-2 and -9. PMID: 12606590
  12. Contractile responses of normal LV rings were measured in the presence and absence of CIMP. CIMP shifted the pressure-length relationship to the left, attenuated LV dilatation, and had no effect on CaCl2-mediated contraction [CIMP] PMID: 15117845

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Subcellular Location
Secreted.
Protein Families
Protease inhibitor I35 (TIMP) family
Tissue Specificity
Expressed in brain, heart, ovary and skeletal muscle.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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