Recombinant Mouse Mitochondrial Rho GTPase 1 (Rhot1), partial

Code CSB-YP805245MO
MSDS
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Source Yeast
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Code CSB-EP805245MO
MSDS
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Source E.coli
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Code CSB-EP805245MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP805245MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP805245MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rhot1
Uniprot No.
Alternative Names
Rhot1; Arht1; Mitochondrial Rho GTPase 1; MIRO-1; EC 3.6.5.-; Ras homolog gene family member T1
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
Gene References into Functions
  1. MIRO1 is an adaptor for microtubule-dependent peroxisome motility in cultured cells. MIRO1 is targeted to peroxisomes and alters their distribution and motility. PMID: 29364559
  2. both Miro proteins can act to coordinate microtubule-dependent and actin-dependent mitochondrial trafficking, Miro1 preferentially acts to control microtubule-dependent trafficking through kinesin and dynein, while Miro2 plays a more prominent role in coordinating mitochondrial interactions with the actin cytoskeleton through a more efficient recruitment and stability of endogenous Myo19 in the mitochondrial membrane. PMID: 29311115
  3. Using mouse knockout strategies, we demonstrate that Miro1, as opposed to Miro2, is the primary regulator of mitochondrial transport in both axons and dendrites. Miro1 deletion leads to depletion of mitochondria from distal dendrites but not axons, accompanied by a marked reduction in dendritic complexity. Disrupting postnatal mitochondrial distribution in vivo by deleting Miro1 in mature neurons causes a progressive loss PMID: 27705781
  4. Data establish that Miro1-mediated mitochondrial positioning at the leading edge provides localized energy production that promotes cell migration by supporting membrane protrusion and focal adhesion stability. PMID: 28615318
  5. This study demonstrated that Miro1 regulates trafficking of mitochondria in the processes of astrocytes, as well as retention of mitochondria at sites that require energy production and Ca2+-buffering such as the tripartite synapse PMID: 26631479
  6. Neuron-specific loss of Miro1 causes depletion of mitochondria from corticospinal tract axons and progressive neurological deficits mirroring human upper motor neuron disease. PMID: 25136135
  7. Miro1 overexpression leads to increased stem cell repair. PMID: 24431222
  8. Armcx genes regulate mitochondrial trafficking in neurons and interact with Miro 1/2 and Trak2. PMID: 22569362

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Subcellular Location
Mitochondrion outer membrane; Single-pass type IV membrane protein.
Protein Families
Mitochondrial Rho GTPase family
Database Links

KEGG: mmu:59040

UniGene: Mm.489674

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