Recombinant Mouse Nicotinamide riboside kinase 1 (Nrk1)

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Code CSB-EP835569MO
MSDS
Size $388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Nrk1
Uniprot No.
Research Area
Metabolism
Alternative Names
NRK 1;NmR-K 1;Nicotinic acid riboside kinase 1;Ribosylnicotinamide kinase 1;RNK 1;Ribosylnicotinic acid kinase 1
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
1-195aa
Target Protein Sequence
MKRFVIGIGGVTNGGKTTLAKSLQKHLPNCSVISQDDFFKPESEIDIDENGFLQYDVLEALNMEKMMSAVSCWMENPGSSAGPAALESAQGVPILIIEGFLLFNYKPLDTIWNRSYFLTVPYEECKRRRSTRVYEPPDPPGYFDGHVWPMYLKHRQEMSSITWDIVYLDGTRSEEDLFSQVYEDVKQELEKQNGL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
26.4 kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN).
Gene References into Functions
  1. NRK1 KO myotubes revealed redundancy in the NRK1/2 dependent metabolism of nicotinamide riboside to NAD+. PMID: 28752046
  2. adenoviral delivery of NRK1 in diet- and age-induced mice elevates hepatic NAD(+) levels, reduces hepatic steatosis, and improves glucose tolerance and insulin sensitivity. PMID: 29678570
  3. nicotinamide riboside kinase 1 (NRK1) is necessary and rate-limiting for the use of exogenous nicotinamide riboside and nicotinamide mononucleotide for NAD(+) synthesis. PMID: 27725675
Protein Families
Uridine kinase family, NRK subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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