Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
Nmes1Normal mucosa of esophagus-specific gene 1 protein
Species
Mus musculus (Mouse)
Target Protein Sequence
MGVFQILMKNKELIPLAFFISVAATGATSFALYALKKTDVVIDRKRNPEPWEMVDPTQPQKLITINQQWKPVEELQKVRRATR
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 1-83 form the expressed segment for recombinant Mouse Nmes1. The theoretical molecular weight of the Nmes1 protein is 25.6 kDa. Expression of this Nmes1 protein is conducted in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the Nmes1 encoding gene fragment was conducted, allowing for easier detection and purification of the Nmes1 protein in subsequent stages.
Mouse normal mucosa of esophagus-specific gene 1 protein (Nmes1) plays a crucial role in cellular differentiation and tissue homeostasis. Its primary function involves contributing to the maintenance of normal esophageal mucosal integrity. In molecular biology, Nmes1 is implicated in regulating gene expression patterns associated with tissue specificity. Exploring Nmes1 provides insights into esophageal biology, cellular differentiation, and potential implications in gastrointestinal research. Additionally, Nmes1's role in tissue homeostasis suggests relevance in understanding epithelial integrity in various organs. Investigating Nmes1 offers avenues for research in molecular and cellular biology, potentially enhancing the understanding of tissue-specific gene regulation and contributing to advancements in gastroenterology.