Recombinant Mouse Osteopontin(Spp1),partial

Code 022603MO
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Spp1
Uniprot No. P10923
Research Area Others
Alternative Names Spp1; Eta-1; Op; Spp-1; Osteopontin; 2AR; Bone sialoprotein 1; Calcium oxalate crystal growth inhibitor protein; Early T-lymphocyte activation 1 protein; Minopontin; Secreted phosphoprotein 1; SPP-1
Species Mus musculus (Mouse)
Source E.coli
Expression Region 17-290aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 34.3kDa
Protein Length Partial
Tag Info N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.; FUNCTION
Gene References into Functions
  1. The data of this study suggest that OPN is essential for proper astrocytic generation in vitro culture prepared from mouse cerebral cortex. PMID: 30246619
  2. Study shows that potent pro-inflammatory and pro-fibrotic molecules, osteopontin and galectin-3, are not major disease modulators of laminin alpha2 chain-deficient muscular dystrophy. PMID: 28281577
  3. Osteopontin activates the NF-kappaB pathway and accelerates the transfer and phosphorylation of p-NF-kappaB P65 from the cytoplasm to the nucleus. In the nucleus, p-NF-kappaB P65 regulates the transcription of genes encoding bone transcription factors, affecting osteogenesis and osteoclast synthesis and the secretion of bone-damaging factors, and ultimately leading to bone destruction. PMID: 30184545
  4. Study demonstrates that osteopontin has an essential role in modulating macrophage immunological profile and their ability to resist pathogenic forms of amyloid beta-protein. PMID: 28860067
  5. BSP and pyrophosphates work in concert to direct mineralization in cementum and likely other mineralized tissues. PMID: 28866368
  6. OPN deficiency has a protective effect against the progressive lipid deposition and glomerulosclerosis elicited by hypercholesterolemia. PMID: 27353458
  7. The present study demonstrated that OPN deficiency reduced intestinal absorption of cholesterol by suppressing the expression of NPC1L1, thus protecting mice from cholesterol gallstone formation. PMID: 28627641
  8. OPN regulates CYP7A1 levels and the metabolic fate of liver acetyl-CoA as a result of CHOL and PC metabolism interplay PMID: 28754826
  9. These results demonstrate that OPN expressed by fatigue-resistant/slow motor neurons is involved in the second-wave neurodegeneration by up-regulating MMP-9 through alphavbeta3 integrin in the mouse model of amyotrophic lateral sclerosis. PMID: 27264390
  10. Osteopontin is highly induced in carbon nanotube-exposed lungs and plays critical roles in TGF-beta1 signaling activation and myofibroblast differentiation to promote fibrosis development. PMID: 28595626
  11. The findings reveal that hepatic OPN contributes to cholesterol gallstone formation by regulating biliary metabolism and might be developed as a therapeutic target for gallstone treatments. PMID: 27484115
  12. Endogenous OPN emerges as a key player in the pathogenesis of chronic Chagas heart disease, through the upregulation of myocardial CCL5/MMP-2 expression and activities resulting in pro-inflammatory and pro-hypertrophic events, cardiac remodeling and interstitial fibrosis. PMID: 28987763
  13. In this study, the effect of deficiency of OPN on intestinal tumor development in Apc-deficient Min mice was investigated.At 16 weeks of age, the number of small intestinal polyps in Min/OPN(+/-) and Min/OPN(-/-) mice was lower than that of Min/OPN(+/+) mice. Colorectal tumor incidences and multiplicities in Min/OPN(+/-) and Min/OPN(-/-) mice were significantly lower than those in Min/OPN(+/+) mice PMID: 28505114
  14. Osteopontin RNA aptamer is a novel and effective approach for preventing cardiac hypertrophy and fibrosis, improving cardiac function, and reversing pressure overload-induced heart failure. PMID: 28453726
  15. OPN deficiency reduced the incidence of chemically induced HCC by suppressing EGFR-mediated anti-apoptotic signaling. PMID: 27888617
  16. In experimental autoimmune encephalomyelitis treated with interferon-beta, OPN was significantly decreased in splenocyte supernatants. Neutralizing OPN antibodies offset the inhibitory effect of IFN-beta on Th17 cells. IFN-beta influenced the acetylation of the Opn gene promoter. IFN-beta plays a role in Th17 differentiation partly through the inhibition of OPN. PMID: 29127843
  17. Osteopontin plays a key role in vascular smooth muscle cell via signaling pathways involving AP-1 and C/EBP beta, leading to increases in vascular smooth muscle cell proliferation and subsequent vascular remodeling. PMID: 27089830
  18. we disclosed the role of chemotaxis and apoptosis in the fibrotic microenvironment-enhanced seeding of tumor cells and identified the fibroblast-derived FN1 and SPP1 as the central mediators in these processes PMID: 27329720
  19. Data show that, together, osteocalcin (OC) and osteopontin (OPN) play important roles in determining bone size, shape, and strength. PMID: 29044594
  20. Results show that BSP and OPN are present in the mineralized tissues of fibrocartilaginous enthuses. PMID: 26826499
  21. AMPK was sufficient to stimulate osteogenesis of MC3T3-E1 cells and inhibit adipogenesis of 3T3-L1 cells through the AMPK-Gfi1-OPN axis. PMID: 27283242
  22. Excess osteopontin exacerbated sarcolemmal injury, and correspondingly, that loss of osteopontin reduced injury extent both in isolated myofibers and in muscle. PMID: 29065150
  23. A MMP-9-cleaved OPN fragment, OPN-32kDa, was responsible for inducing expansion of myeloid-derived suppressor cells, which may contribute to 3LL tumor's evasion of the immune response. PMID: 28986261
  24. OPN played a critical role in activating the migration of Mesenchymal stem cells to injured sites and their differentiation into specific skin cell types during skin wound healing. PMID: 28957406
  25. Findings demonstrate that claudin-low mammary tumor cells rely on OPN for proliferation, survival and migration as knockdown of OPN using siRNA inhibited proliferation and migration while increasing apoptosis. PMID: 27282619
  26. Intracellular OPN (iOPN) diminished the population size of myeloid progenitor cells and myeloid cells, and secreted OPN (sOPN) increase the population size of lymphoid cells. The total effect of OPN on skewing the leukocyte population balance was observed as host sensitivity to early systemic infection with Candida albicans and T cell-mediated colitis. PMID: 28671690
  27. this study shows that OPN promotes sepsis in Pseudomonas aeruginosa-infected mice and potentially blocks B cell-dependent immunity PMID: 28601359
  28. data suggest the possibility that Opn might regulate the homeostasis of intestinal microflora through maintenance of TCRgammadelta+ IELs, possibly by support of IEL survival PMID: 28296922
  29. OPN secreted by follicular CD153(+) senescence-associated T cells in germinal centers (GCs) promotes a continuous supply of intracellular autoantigens via apoptotic cells, thus playing a key role in the progression of the autoreactive GC reaction and leading to pathogenic autoantibody production in lupus-prone mice. PMID: 27534552
  30. study demonstrated an anti-tumorigenic role of OPN in transgenic adenocarcinoma of the mouse prostate (TRAMP) development and also suggests that the contribution of OPN to tumor development depends on the type of tumor as well as the source and isoform of OPN PMID: 27601131
  31. These data suggest a critical role for reduced stroma-derived osteopontin for hematopoietic stem cell aging. PMID: 28254837
  32. Osteopontin is a key mediator released by senescent pulmonary artery smooth muscle cells and contributes to pulmonary hypertension progression. PMID: 27444202
  33. Osteopontin (OPN) plays an anti-inflammatory role in acute gastrointestinal acute graft-versus-host disease (GVHD). PMID: 28192120
  34. Circulating OPN levels are upregulated in human and murine acute schistosomiasis. PMID: 27755536
  35. essential for the type I collagen secretion by newly differentiated odontoblast-like cells to form reparative dentin PMID: 27126446
  36. this study shows that neutrophil accumulation at the site of infection with a mixture of endodontic pathogens is significantly reduced in OPN-deficient mice, and is accompanied by an increase in bacterial load PMID: 27599164
  37. OPN ablation skews macrophage polarization toward a pro-regenerative phenotype. PMID: 27091452
  38. OPN induces oxidative stress via the involvement of mitochondria and NOX-4. It may affect mitochondrial morphology and integrity, at least in part, via the involvement of BIK. PMID: 27262843
  39. Endogenous osteopontin plays a critical role in the prevention of phosphate-induced nephrocalcinosis and vascular calcification in response to high phosphate load. PMID: 27083280
  40. GATA4 acts as a negative regulator of Bsp expression in osteoblasts. PMID: 26973342
  41. Genetic deletion of osteopontin in TRAMP mice skews prostate carcinogenesis from adenocarcinoma to aggressive human-like neuroendocrine cancers. PMID: 26700622
  42. IFN-gamma regulates hypertensive vascular collagen remodeling induced by ANG II and inhibits the differentiation of Th17 cells by suppressing OPN expression and regulating secretion of inflammatory cytokines. PMID: 26823760
  43. results indicate that autocrine OPN plays a crucial role in HPC expansion, migration, and subsequent oncogenic transformation of HPCs, which may provide a new insight into hepatocarcinogenesis PMID: 26033745
  44. OPN produced by hematogenous macrophages induces astrocyte process extension toward the infarct border zone, which may contribute to repair of the ischemic neurovascular unit following stroke PMID: 26148976
  45. During the priming phase of liver regeneration, osteopontin enhances the recruitment of macrophages and neutrophils, and triggers hepatocyte proliferation through Kupffer cell-derived IL-6 release and the downstream activation of Stat3. PMID: 26327817
  46. OPN deficiency accelerated the spontaneous development of colitis in mice with disrupted gut microbiota and macrophage phagocytic activity. PMID: 26274807
  47. mechanical stretch (MS) directly increased OPN protein expression and secretion in vascular smooth muscle cells. PMID: 25986148
  48. OPN may modulate psoriasis-like inflammation through altering lymphocyte distribution in skin and draining lymph nodes and by inducing IL-17 expression of inflammatory T cells. PMID: 25655893
  49. Osteopontin is expressed in the oviduct and promotes fertilization and preimplantation embryo development. PMID: 25263084
  50. BSP has a role in mouse cementum and craniofacial bone mineralization PMID: 25963390
  51. OPN actually contributes to the development of gastric cancer. PMID: 26438603
  52. Hypergravity promoted OPN expression in osteoblasts by facilitating focal adhesion assembly, strengthening actin bundles, and increasing Runx2 expression. PMID: 26046934
  53. lentiviral-OPN short hairpin RNA ameliorates the inflammatory response and bone destruction in mice with collagen-induced arthritis. Therefore, OPN may be a potential therapeutic target for rheumatoid arthritis. PMID: 26304992
  54. Data show that liver and serum osteopontin (OPN) levels correlated with fibrosis stage. PMID: 26201095
  55. Hindlimb-unloading increases OPN expression in circulating cells and suppresses bone marrow B-lymphogenesis PMID: 24831120
  56. A novel motif, LRSKSRSFQVSDEQY, in the C-terminal fragment of MMP-3/7-cleaved mouse OPN binds to alpha9beta1 integrin. PMID: 25545242
  57. local OPN expression combined with a peripheral inflammatory stimulus, such as pertussis toxin, may be implicated in the development of brain inflammation and induction of cell death PMID: 25525298
  58. Studied the involvement of OPN in Helicobacter pylori (HP)-induced gastritis using OPN knockout (KO) mice and OPN knockdown (KD) cell lines. PMID: 25867766
  59. BSP is an important and multifaceted protein that regulates both chondrocyte proliferation and apoptosis as well as transition from cartilage to bone during development of endochondral bone. PMID: 25464126
  60. Our study demonstrates that the sOPN/integrin alphav axis, which induces T cell chemotaxis toward macrophages, is critical for suppressing hyperinflammation during the first 3 h of endotoxemia. PMID: 25972484
  61. Together, our findings define a novel, ovary-dependent mechanism underlying gender-specific augmentation of emphysema through transcriptional control of the Opn gene. PMID: 25912141
  62. osteopontin silencing in tumor cells delayed both tumor growth and extramedullary myelopoiesis PMID: 25273090
  63. Evidence from several genetic mouse models suggests that OPN is an enhancer of atherosclerosis. Review. PMID: 25128758
  64. ablation of the OPN gene, Spp1, leads to improvements in the skeletal phenotype in Phospho1(-/-) as they age PMID: 24825455
  65. Activation of PI3K (phosphatidylinositol 3-kinase)-AKT pathway was required for OPN-induced brown adipogenesis. The findings suggest OPN plays an important role in promoting the differentiation of brown adipocytes. PMID: 25749339
  66. Osteopontin deletion prevents the development of obesity and hepatic steatosis via impaired adipose tissue matrix remodeling and reduced inflammation and fibrosis in adipose tissue and liver in mice. PMID: 24871103
  67. OPN is a key mediator of the alcohol-induced effects on hepatic stellate cell functions and liver fibrogenesis. PMID: 25278703
  68. suggesting that the effects might in part reflect the higher OPN expression in the absence of BSP PMID: 25502698
  69. retinal ganglion cells accounted for nearly all regeneration following downregulation of PTEN, which activates the mTOR pathway PMID: 25754821
  70. PGC-1alpha drives functional angiogenesis in skeletal muscle via SPP1 induction and macrophage activation. PMID: 25009290
  71. Data (including data from knockout mice) suggest that osteopontin (Spp1) plays regulatory role in promoting healing/neovascularization of diabetic skin wounds (here, mesenchymal fetal stem cell transplantation was treatment used). PMID: 24827928
  72. deficiency of osteopontin in NK cells results in defective responses to IL-15 associated with an increase in NK cell contraction phase of homeostatic expansion, defective expression of Eomes transcription factor, and diminished response to tumors PMID: 25550515
  73. Data indicated that OPN expression in the mouse uterus during early pregnancy is essential for blastocyst hatching and adhesion and that the knockdown of OPN in mouse endometrial stroma cells could lead to a restrained in vitro trophoblast invasion. PMID: 25133541
  74. Osteopontin in hepatocytes inhibits acetaminophen metabolism, while osteopontin in macrophages enhances acetaminophen toxicity. PMID: 22728710
  75. protective role in alcoholic hepatitis PMID: 25132354
  76. Intracellular osteopontin protects Bcl-6 from degradation downstream of signaling via the receptor ICOS in both follicular helper and regulatory T cells. PMID: 25436971
  77. Osteopontin (OPN) plays an important role in the regulation of biomineralization, but does not appear to affect osteoblast cell development in vitro. PMID: 25310312
  78. Chronic UVB-induced OPN expression protects the survival of initiated basal keratinocytes and, consequently, facilitates cutaneous squamous carcinoma development. PMID: 24888687
  79. Expression of Opn was upregulated in mouse ovarian granulosa cells in response to a gonadotropin surge through epidermal growth factor receptor signaling. PMID: 25352284
  80. cell stress induces the upregulation of OPN in AEC II by signaling through the ERK pathway PMID: 24963635
  81. full expression of either OPN or BSP is necessary for the anabolic effect of PTH at least in the ectopic calvaria injection model PMID: 25160656
  82. Results reveal that OPN promotes the progression of gastric cancer through the NF-kappaB pathway. PMID: 24756245
  83. Osteopontin is a matricellular protein involved in ductal reaction and contributing to scarring and liver fibrosis via TGF-beta. PMID: 24496779
  84. Microarray analysis suggests a dysregulation of key lung developmental transcripts in gene-targeted Spp1((-/-)) mice, particularly during the peak phase of alveologenesis. PMID: 24816281
  85. Thrombin cleavage of osteopontin disrupts a pro-chemotactic sequence for dendritic cells, which is compensated by the release of its pro-chemotactic C-terminal fragment. PMID: 25112870
  86. This study demonstrates that OPN can mediate immune response during TBI synaptic repair, positively influencing synapse reorganization and functional recovery. PMID: 25151457
  87. The CD44 ligand osteopontin shared a perivascular expression pattern with CD44 and promoted glioma stem cell-like phenotypes. PMID: 24607407
  88. Hepatic ischemia reperfusion injury is aggravated in osteopontin knockout mice. PMID: 24810044
  89. increased OPN levels were significantly decreased upon lowering serum phosphate by feeding a low-phosphate diet or after deletion of NaPi2a, indicating that phosphate levels contribute in part to the high OPN levels in Fgf23(-/-) mice PMID: 24038141
  90. our study demonstrates a novel role for OPN-integrin av/b1 in regulating MSC differentiation. PMID: 24123709
  91. These data provide compelling evidence that OPN and COX-2 expressing macrophages are obligatory factors in melanoma growth. PMID: 23728342
  92. osteopontin, acting via CD44 receptors, induces apoptosis in myocytes via the involvement of mitochondrial death pathway and endoplasmic reticulum stress PMID: 24531809
  93. our results suggest that osteopontin expressed by tumor-derived microparticles play an important role in bone-marrow derived pro-angiogenic cell mobilization and colonization of tumors PMID: 24347266
  94. contribution of OC-OPN to fracture toughness is related to their presence at the extrafibrillar organic-mineral interfaces PMID: 24128197
  95. OP has crucial role in blastocyst implantation; its co-expression with beta3 integrin is a biological marker for good endometrial receptivity PMID: 23870190
  96. Disrupting the interaction of a specific domain of the Opn protein responsible for its CD103(-) DC-mediated proinflammatory effect, with integrin alpha9, overexpressed on colitic CD103(-) DCs, suppressed the inflammatory potential of these cells. PMID: 24550510
  97. These studies suggest unique roles for SC-derived OPN and CLU in regeneration of peripheral motor and sensory axons. PMID: 24478351
  98. This study establish at least in part, the molecular mechanism underlying the role of host OPN in melanoma growth and angiogenesis. PMID: 23935934
  99. BAFF regulates T-cell survival by inducing osteopontin secretion in B cells in autoimmune diseases. PMID: 24084099
  100. OPN is a sexually dimorphic modifier of muscle size in normal humans and mice and responds to muscle damage. PMID: 23274598

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Subcellular Location Secreted
Protein Families Osteopontin family
Database Links

KEGG: mmu:20750

STRING: 10090.ENSMUSP00000084043

UniGene: Mm.288474

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