Recombinant Mouse POU domain, class 5, transcription factor 1 (Pou5f1)

Code CSB-YP018403MO
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Source Yeast
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Code CSB-EP018403MO
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Source E.coli
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Code CSB-EP018403MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018403MO
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Source Baculovirus
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Code CSB-MP018403MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pou5f1
Uniprot No.
Alternative Names
Pou5f1; Oct-3; Oct-4; Otf-3; Otf3; POU domain; class 5; transcription factor 1; NF-A3; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3
Species
Mus musculus (Mouse)
Expression Region
1-352
Target Protein Sequence
MAGHLASDFA FSPPPGGGDG SAGLEPGWVD PRTWLSFQGP PGGPGIGPGS EVLGISPCPP AYEFCGGMAY CGPQVGLGLV PQVGVETLQP EGQAGARVES NSEGTSSEPC ADRPNAVKLE KVEPTPEESQ DMKALQKELE QFAKLLKQKR ITLGYTQADV GLTLGVLFGK VFSQTTICRF EALQLSLKNM CKLRPLLEKW VEEADNNENL QEICKSETLV QARKRKRTSI ENRVRWSLET MFLKCPKPSL QQITHIANQL GLEKDVVRVW FCNRRQKGKR SSIEYSQREE YEATGTPFPG GAVSFPLPPG PHFGTPGYGS PHFTTLYSVP FPEGEAFPSV PVTALGSPMH SN
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Gene References into Functions
  1. Oct4 is involved in DNA methylation through the regulation of Dnmt1 transcription, especially during the early stages of mouse pre-implantation embryo development. PMID: 30140294
  2. Zfp127 is a novel regulator of Oct4, and may become a potent target to improve cellular reprogramming. PMID: 29335068
  3. Here we describe an optimized DamID method coupled with next-generation sequencing (DamID-seq) in mouse cells and demonstrate the identification of the binding sites of two TFs, POU5F1 (also known as OCT4) and SOX2, in as few as 1000 embryonic stem cells (ESCs) and neural stem cells (NSCs), respectively. PMID: 29572359
  4. These results indicate a role for Oct4 in orchestrating multiple fates and enabling expansion, correct patterning and lineage choice in the postimplantation epiblast. PMID: 29915126
  5. Acidic pH induced OCT-4 expression in fibroblasts and stromal cells in tumor microenvironment. PMID: 27302093
  6. Threonine(343) based OCT4-phosphorylation is crucial for the maintenance of ESC pluripotency and interaction with SOX2. PMID: 28988986
  7. These results demonstrated that OCT4 phosphorylation on serine 347 by JNKs plays an important role in its stability, transcriptional activities, and self-renewal of mouse ESCs PMID: 29153991
  8. the epigenetic status of Oct4 and Nanog genes confirm that pre-induced pluripotent stem cells belong to an RVTg+OCT4+NANOG- state PMID: 27622636
  9. These observations support a role for YY1 meditating and/or regulating the interaction of OCT4 and SOX2 at a posttranscriptional level. PMID: 28682643
  10. The results revealed that the synthesized complex decoy can concomitantly target Sox2 and Oct4, which subsequently represses the stemness properties of mESCs compared to controls through decreasing cell viability, arresting cell cycle in G0 /G1 phases, inducing apoptosis, and modulating differentiation in mESCs despite the presence of 2i/LIF in cell culture. PMID: 28833847
  11. Tip110 deletion impaired embryonic and stem cell development involving downregulation of stem cell factors Nanog, Oct4, and Sox2. PMID: 28436127
  12. Nanog, Oct4 and Tet1 interplay in establishing stem cell pluripotency has been described. PMID: 27146218
  13. Chromatin accessibility at OCT4-bound sites requires the chromatin remodeller BRG1, which is recruited to these sites by OCT4 to support additional transcription factor binding and expression of the pluripotency-associated transcriptome. PMID: 28287392
  14. data infer that OCT4 expression may have a direct effect on partial cardiomyocyte reprogramming of MSCs and suggest a new mechanism(s) associated with MSC multipotency and a requirement for crosstalk with the cardiac microenvironment PMID: 29216265
  15. Regulation of Oct4 by SirT1 may link stem cell development to environmental conditions, and it may provide strategies to manipulate epiblast cell state. PMID: 27732856
  16. we suggest that the activity of Oct4 DE and PE is regulated by the repressive histone marks and DNA methylation in a cell-type-specific manner. PMID: 28157483
  17. PRDM14 recruited OCT3/4 to the enhancer regions of naive pluripotency genes via TET-base excision repair-mediated demethylation. PMID: 27866876
  18. High POU5F1 expression is associated with disruption in differentiation and spermatogenesis. PMID: 27486267
  19. Three regulating complexes centered by Sox2-Oct4, Nanog, and Lrh1 maintain Oct4 expression in pluripotent stem cells in mice. [review] PMID: 28731785
  20. OCT4 is an integral and necessary component of signal-regulated transcriptional processes required for tissue-specific responses. PMID: 27499297
  21. Maintenance of pluripotency is regulated by a network of transcription factors coordinated by Oct4, Sox2, and Nanog; Trim24 significantly improved efficiency of cellular reprogramming, demonstrating its direct functionality in establishing pluripotency. PMID: 27773674
  22. ATOX1 appeared ubiquitously expressed throughout the cells until compaction; in subsequent embryo stages, ATOX1 relocalized to cytoplasmic perinuclear domains in the inner cell mass. Silencing of Oct4 did not affect Atox1 expression, but silencing of Atox1 at the 2-cell stage strongly diminished Oct4 expression in 16-cell embryos. PMID: 28711491
  23. smooth muscle cell (SMC)-specific conditional knockout of Oct4 in Apoe-/- mice resulted in increased lesion size and changes in lesion composition that are consistent with decreased plaque stability, including a thinner fibrous cap, increased necrotic core area, and increased intraplaque hemorrhage. PMID: 27183216
  24. In mouse model of acute uterine injury, Nanog homebox (NANOG) expression reached a peak at 6 h, while sex-determining region Y-box2 (SOX2) and octamer-binding protein 4 (OCT4) peaked later at 12 h after lipopolysaccharide (LPS) treatment. PMID: 28253866
  25. Here, the authors present the first structure of mouse GCNF DNA-binding domain in complex with the Oct4 DR0. The overall structure revealed two molecules bound in a head-to-tail fashion on opposite sides of the DNA at a DR0 motif located within the Oct4 promoter. PMID: 27984042
  26. To our knowledge, this is the first report on lineage reprogramming of TILs using protein stem cell transcription factors delivered directly to the nucleus. PMID: 27084449
  27. In late preimplantation, Oct4 served as a chromatin opener to create transcriptional permissive states at Xm-Xist/Tsix genomic loci. In parthenogenetic embryos, Rnf12 overdose caused Xm-Xist derepression via Xm-Tsix repression; physiological Rnf12 levels were essential for Xm-Xist silencing maintenance in fertilized embryos. PMID: 27788132
  28. Critical POU domain residues confer Oct4 uniqueness in somatic cell reprogramming. PMID: 26877091
  29. Hierarchical Oct4 binding in concert with primed epigenetic rearrangements during somatic cell reprogramming has been described. PMID: 26832419
  30. One-week administration of valproic acid followed by one-week forced expression of Oct4 enhanced myelination by converting transduced cells to myelinating oligodendrocytes PMID: 26804242
  31. Aurkb phosphorylates Oct4(S229) during G2/M phase, leading to the dissociation of Oct4 from chromatin, whereas PP1 binds Oct4 and dephosphorylates Oct4(S229) during M/G1 transition, which resets Oct4-driven transcription for pluripotency and the cell cycle. PMID: 26880562
  32. Bcl3 Bridges LIF-STAT3 to Oct4 Signaling in the Maintenance of Naive Pluripotency. PMID: 26303070
  33. Oct3/4 plays a crucial role in mediating CSCs to induce cardiac regeneration. PMID: 26704423
  34. OCT4-SOX2 configuration that dimerizes on a Hoxb1-like composite, a canonical element with juxtaposed individual binding sites, plays a more critical role in the induction and maintenance of pluripotency. PMID: 26314899
  35. The long noncoding RNA Gm15055 is highly expressed in mouse embryonic stem cells and its expression is maintained by OCT4. PMID: 26615201
  36. The Oct4 distal enhancer interacts with genomic loci that exhibit open chromosome features and contain active histone marks. PMID: 26435056
  37. In summary, the data presented here reveal that TRIM32 directly regulates at least two of the four Yamanaka Factors (cMyc and Oct4), to modulate cell fate transitions. PMID: 26307407
  38. Tgif1 counterbalances the activity of core pluripotency factors, Oct4, Sox2, and Nanog, in mouse embryonic stem cells. PMID: 26411691
  39. Pluripotency transcription factors Nanog and Oct4 positively modulate Sod2 gene transcription in induced pluripotent stem cells. PMID: 26642061
  40. Results suggest that Oct4 and Sox 2 transcription factors can decrease nucleosome occupancy in vivo at poised genes, but do not alter in vivo nucleosome occupancy at active genes. PMID: 25992972
  41. This data highlights a crucial role for Oct4A in the progression and metastasis of EOC. Targeting Oct4A may prove to be an effective strategy in the treatment and management of epithelial ovarian tumours. PMID: 26260289
  42. YAP1 regulates OCT4 activity and SOX2 expression to facilitate self-renewal of stem-like cell PMID: 25754111
  43. findings demonstrate that chromatin organization and transcriptional programs are intimately connected in ESCs and that the dynamic positioning of the Oct4 alleles is associated with the transition from pluripotency to lineage specification. PMID: 25748933
  44. The BET family member BRD4 interacts with OCT4 and regulates pluripotency gene expression PMID: 25684227
  45. Pontin functions as an essential coactivator for Oct4-dependent large intergenic noncoding RNA expression in mouse embryonic stem cells. PMID: 25857206
  46. Reptin plays a key role in maintaining the pluripotency of embryonic stem cells and in establishing the pluripotency during reprogramming of somatic cells by regulation of Oct4-mediated gene regulation PMID: 25185564
  47. The mechanism of hypoxia induction of germ cell pluripotency involves HIF1alpha stabilization and Oct4 deregulation. PMID: 25226357
  48. Study showed that Ezh2 mRNA expression was inversely correlated with Oct4 and Sox2, but Oct4 and Sox2 negatively affect Ezh2; additionally, Oct4 and Sox2 were negative regulators of Ezh2 primarily on a post-translation level. PMID: 25501223
  49. OCT4 and SOX2 are the primary transcription factors recruiting SCC to regulatory regions of pluripotency genes; the XPC subunit is essential for interaction with the two proteins PMID: 25901318
  50. Oct3/4 directly bound to the promoter region of the E2F3a gene and positively regulated expression of E2F3a in embryonic stem cells. PMID: 25727014

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
POU transcription factor family, Class-5 subfamily
Tissue Specificity
Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues.
Database Links
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