Recombinant Mouse Phosphoinositide 3-kinase regulatory subunit 6 (Pik3r6)

Code CSB-YP664932MO
MSDS
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Source Yeast
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Code CSB-EP664932MO
MSDS
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Source E.coli
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Code CSB-EP664932MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP664932MO
MSDS
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Source Baculovirus
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Code CSB-MP664932MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Pik3r6; Phosphoinositide 3-kinase regulatory subunit 6; Phosphoinositide 3-kinase gamma adapter protein of 87 kDa; p84 PI3K adapter protein; p84 PIKAP; p87 PI3K adapter protein; p87PIKAP
Species
Mus musculus (Mouse)
Expression Region
1-756
Target Protein Sequence
MESSDVELDF QRSVQAVLRE LNTPNPALQS NQGMWRWSLH KKVERNPGKS SILVRILLRE LEKAESEDGR RVIIPLLLTL MSVLTKATGI PEDLYHRAYT FCTRLLTLPA PYSTVALDCA IRLKTETAVP GTLYQRTVIA EQNLISELYP YQERVFLFVD PELVSASVCS ALLLEIQAAQ EQQTPEACMR HVVSHALQAA LGEACHTGAL NRKLQASSRR VLEYYFHAVV AAIEQVASED SPSRLGHLEK MEEIYCSLLG PATTRRHCVG DLLQDRLPSI PLPSPYITFH LWTDQEQLWK ELVLFLRPRS QLRLSADLDA LDLQGFRLDR DLARVSTDSG IERDLPLGSD ELPDPSSSEM ERAALQRKGG IKKRVWPPDF FMPGSWDGPP GLHRRTGRPS GDGELLPGVS RVHTARVLVL GDDRMLGRLA QAYYRLRKRE TKKFCLTPRL SLQLYYIPVL APQVTGQDPE ASRKPELGEL ASFLGRVDPW YESTVNTLCP AILKLAEMPP YLDTSRTVDP FILDVITYYV RMGTQPIYFQ LYKVKIFTSL SHDPTEDIFL TELKVKIQDS KSPKEGSSPR RRGAAEGTGA ELSMCYQKAL LSHRPREVTV SLRATGLVLK AIPAGDTEVS GFFHCTSPNA ASATDCSCLH VSVTEVVKSS NLAGRSFTTS TNTFRTSSIQ VQSQDQRLLT LWLDKDGRRT FRDVVRFEVS PCPEPCSRTQ KSKTSALNSH GQETEKNMAK PNSLLMPINT FSGIIQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulatory subunit of the PI3K gamma complex. Acts as an adapter to drive activation of PIK3CG by beta-gamma G protein dimers. The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-gamma G proteins than PIK3CG:PIK3R5 and its membrane recruitment and beta-gamma G protein dimer-dependent activation requires HRAS bound to PIK3CG. Recruits of the PI3K gamma complex to a PDE3B:RAPGEF3 signaling complex involved in angiogenesis; signaling seems to involve RRAS.
Gene References into Functions
  1. These data demonstrate PI3Kgamma-dependent signalling downstream of IL-17RA, which plays a pivotal role in regulating IL-17 production in T cells. PMID: 22930133
  2. GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4. PMID: 22728827
  3. that genetic deletion of PI3Kgamma enhanced scratching behaviours in histamine-dependent and protease-activated receptor 2 (PAR-2)-dependent itch. PMID: 22168443
  4. p87PIKAP is a novel regulatory subunit of phosphoinositide 3-kinase gamma that is highly expressed in heart and interacts with PDE3B [p87PIKAP] PMID: 16476736
  5. ablation of PI3Kgamma reduced SDF1alpha-induced integrin activation in human EPCs and in murine Lin(-) BM-derived progenitor cells PMID: 18323525
  6. PI3Kgamma has a role in the induction of CXCR3 on T cells PMID: 18658026
  7. Results suggest that the CXCR2 and PI3Kgamma signaling pathway may represent a suitable target for the development of novel therapeutic strategies for human diseases characterized by vascular leakage. PMID: 19255141

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Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein.
Tissue Specificity
Highly expressed in heart. In a lower extent, also expressed in brain, spleen, lung, liver, kidney, prostate, thyroid, salivary gland, dendritic cells, macrophages and neutrophils.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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