Recombinant Mouse Probable ATP-dependent RNA helicase DDX4 (Ddx4)

Code CSB-YP717216MO
MSDS
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Source Yeast
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Code CSB-EP717216MO
MSDS
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Source E.coli
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Code CSB-EP717216MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP717216MO
MSDS
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Source Baculovirus
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Code CSB-MP717216MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ddx4
Uniprot No.
Alternative Names
Ddx4; Mvh; VasaATP-dependent RNA helicase DDX4; EC 3.6.4.13; DEAD box protein 4; Mvh; Vasa homolog
Species
Mus musculus (Mouse)
Expression Region
1-702
Target Protein Sequence
MGDEDWEAEI LKPHVSSYVP VFEKDKYSSG ANGDTFNRTS ASSDIGESSK KENTSTTGGF GRGKGFGNRG FLNNKFEEGD SSGFWKESNN DCEDNQTRSR GFSKRGGCQD GNDSEASGPF RRGGRGSFRG CRGGFGLGRP NSESDQDQGT QRGGGLFGSR KPAASDSGNG DTYQSRSGSG RGGYKGLNEE VVTGSGKNSW KSETEGGESS DSQGPKVTYI PPPPPEDEDS IFAHYQTGIN FDKYDTILVE VSGHDAPPAI LTFEEANLCQ TLNNNIAKAG YTKLTPVQKY SIPIVLAGRD LMACAQTGSG KTAAFLLPIL AHMMRDGITA SRFKELQEPE CIIVAPTREL INQIYLEARK FSFGTCVRAV VIYGGTQFGH SVRQIVQGCN ILCATPGRLM DIIGKEKIGL KQVKYLVLDE ADRMLDMGFG PEMKKLISCP GMPSKEQRQT LLFSATFPEE IQRLAGDFLK SSYLFVAVGQ VGGACRDVQQ TILQVGQYSK REKLVEILRN IGDERTMVFV ETKKKADFIA TFLCQEKIST TSIHGDREQR EREQALGDFR CGKCPVLVAT SVAARGLDIE NVQHVINFDL PSTIDEYVHR IGRTGRCGNT GRAISFFDTD SDNHLAQPLV KVLSDAQQDV PAWLEEIAFS TYVPPSFSSS TRGGAVFASV DTRKNYQGKH TLNTAGISSS QAPNPVDDES WD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.
Gene References into Functions
  1. BHR also decreased the mRNA level of MVH in the bone marrow, and increased mRNA and protein level of MVH in the ovary o fpremature ovarian failure mice PMID: 27465335
  2. Hgher expression levels of ~55-60 kDa MVH isoform in the ovaries were followed by the accumulations of preovulatory follicles. PMID: 27460133
  3. In mouse oocytes the mitochondrion-originated germinal body-like structures accumulate MVH protein. PMID: 24780413
  4. The results indicate the presence of a developmentally regulated mechanism in which Hat1 and p46 control the physiology of the chromatoid body by acetylation of MVH. PMID: 22223882
  5. Vasa/Ddx4 gene can be involved in regulation of ground and primed states of pluripotency PMID: 22288104
  6. SPSB1 and SPSB4 bind strongly to both Par-4 and VASA peptides. PMID: 20561531
  7. MVH plays crucial roles in the early phase of the ping-pong amplification cycle PMID: 20439430
  8. The RNA helicase Vasa is another essential protein in germline development and arginine methylation is conserved across phyla PMID: 20080973
  9. Data show that RanBPM and mouse vasa homolog (Mvh) are closely associated with perinuclear RNA-protein complexes and chromatoid bodies, and suggests a functional relationship between translational regulation and microtubule nucleation during meiosis. PMID: 14648869

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Subcellular Location
Cytoplasm. Cytoplasm, perinuclear region.
Protein Families
DEAD box helicase family, DDX4/VASA subfamily
Tissue Specificity
Testis-specific.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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