Recombinant Mouse Probetacellulin (Btc), partial

Code CSB-MP002853MO
Abbreviation Recombinant Mouse Btc protein, partial
MSDS
Size $138
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Target Names
Uniprot No.
Research Area
Others
Alternative Names
Btc
Species
Mus musculus (Mouse)
Source
Mammalian cell
Expression Region
32-111aa
Target Protein Sequence
DGNTTRTPETNGSLCGAPGENCTGTTPRQKVKTHFSRCPKQYKHYCIHGRCRFVVDEQTPSCICEKGYFGARCERVDLFY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
10.7 kDa
Protein Length
Partial
Tag Info
C-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Gene References into Functions
  1. Betacellulin promotes the proliferation of corneal epithelial stem cells through the phosphorylation of Erk1/Erk2. PMID: 29331377
  2. The results of this study suggested a novel regulatory role of Btc in axon-Schwann cell interactions involved in myelin formation and nerve repair. PMID: 28139851
  3. BTC induces expansion of neural stem cells and neuroblasts, and promotes neurogenesis in the olfactory bulb and dentate gyrus. PMID: 22232668
  4. Betacellulin exerts proliferative activity on beta cells through the activation of ErbB-1 and ErbB-2 receptors PMID: 21897861
  5. BTC overexpression enhances glucose-induced insulin secretion in Mesenchyma Stem Cells PMID: 20836700
  6. Betacellulin protects from pancreatitis by activating stress-activated protein kinase. PMID: 20038432
  7. Excess of betacellulin exerts no deleterious effects on the structure or function of the male reproductive tract in mice. PMID: 19931240
  8. structure of the BTC gene PMID: 12031500
  9. Defective valvulogenesis in HB-EGF and TACE-null mice is associated with aberrant BMP signaling. PMID: 12773386
  10. Mice overexpressing BTC exhibit high early postnatal mortality, reduced body weight gain, impaired longitudinal growth, cataract, retinal and bone abnormalities, and lung disease. PMID: 16179416
  11. identified hitherto unknown effects of betacellulin (BTC) overabundance in reproduction and suggests BTC as a novel candidate protein for the modulation of fertility. PMID: 17914071
  12. role of BTC in the regulation of epidermal homeostasis, hair follicle morphogenesis and cycling, and wound angiogenesis PMID: 17960175
  13. Overexpression of BTC results in an EGFR-dependent upregulation of cortical bone mass in the appendicular skeleton of mice. PMID: 19049329
  14. Long-term BTC overabundance does not alter pancreatic islet structure and beta-cell mass, but enhances glucose-induced insulin secretion in vivo as well as in vitro. PMID: 19100309
  15. Reversal of streptozotocin-induced hyperglycemia by continouous supply of Btc. PMID: 19378417

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Subcellular Location
[Betacellulin]: Secreted, extracellular space.; [Probetacellulin]: Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Found in several mouse tissues including kidney, uterus and liver, as well as in beta tumor cell line and MCF-7 cells. It is not detected in the brain.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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