Recombinant Mouse Profilin-2 (Pfn2)

Code CSB-YP884933MO
MSDS
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Source Yeast
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Code CSB-EP884933MO
MSDS
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Source E.coli
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Code CSB-EP884933MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884933MO
MSDS
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Source Baculovirus
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Code CSB-MP884933MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Pfn2; Profilin-2; Profilin II
Species
Mus musculus (Mouse)
Expression Region
1-140
Target Protein Sequence
MAGWQSYVDN LMCDGCCQEA AIVGYCDAKY VWAATAGGVF QSITPVEIDM IVGKDREGFF TNGLTLGAKK CSVIRDSLYV DGDCTMDIRT KSQGGEPTYN VAVGRAGRVL VFVMGKEGVH GGGLNKKAYS MAKYLRDSGF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Gene References into Functions
  1. RARalpha competes with other PFN2a-binding proteins bearing PRMs and involved in actin filaments elongation. Consequently, the actin filament network is altered and MEFs adhesion is decreased. This novel role opens novel avenues for the understanding of pathologies characterized by increased levels of cytoplasmic RARalpha. PMID: 29158086
  2. Pfn2 expression is controlled by the iron regulatory proteins in vivo and that Pfn2 contributes to maintaining iron homeostasis in cell lines and mice. PMID: 28774878
  3. knockdown of profilin 1 or profilin 2a led to significant decrease in cell spreading of astrocytes. the knockdown of profilin 2a resulted in a significantly reduced morphological complexity of astrocytes in both dissociated and slice culture astrocytes. PMID: 25629407
  4. Hyper-phosphorylation of profilin2a is the molecular link between SMN and the ROCK pathway repressing neurite outgrowth in neuronal cells. PMID: 21920940
  5. Profilin II regulates the exocytosis of kainate glutamate receptors. PMID: 20937818
  6. Phosphatidylinositol 4,5-bisphosphate releases Pfn2 from the Pfn2-dynamin 1 complex as well as from the Pfn2-actin complex, suggesting that Pfn2 is diverging the phosphoinositide signaling pathway to actin polymerization as well as endocytosis PMID: 16319076
  7. Following NMDA receptor activation profilin 2 accumulates not only in dendritic spines, but also in the nucleus of hippocampal neurons via a process involving rearrangement of the actin cytoskeleton. PMID: 16716297
  8. results highlight a novel, profilin2-dependent pathway, regulating synaptic physiology, neuronal excitability, and complex behavior PMID: 17541406
  9. High-resolution structural analysis of mouse profilin 2a complex formation with two physiological ligands: the formin homology 1 domain of mDia1 and the proline-rich domain of VASP. PMID: 18001770

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Profilin family
Tissue Specificity
Isoform IIa is the main isoform and is abundant in brain. Isoform IIb is a minor isoform.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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