Recombinant Mouse Protein DBF4 homolog A (Dbf4)

Code CSB-YP865604MO
MSDS
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Source Yeast
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Code CSB-EP865604MO
MSDS
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Source E.coli
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Code CSB-EP865604MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865604MO
MSDS
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Source Baculovirus
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Code CSB-MP865604MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dbf4
Uniprot No.
Alternative Names
Dbf4; Dbf4aProtein DBF4 homolog A; MuDBF4
Species
Mus musculus (Mouse)
Expression Region
1-663
Target Protein Sequence
MNLETMRIHS KAPLPGGIQD RNEKNRPSLK SLKADNRLEK SKYKPLWGKI FYLDLPSITI CEKLQKDIKE LGGRVEEFLS KDISYFVSNK KEAKYAQTLG RVSPVPSPES AYTAETTSPH PSHDGSSFKS QDRVCLSRGK LLAEKAVKDH DFIPANSILS NALSWGVKIL HIDDIRYYIE QKKKALSALK KSSASGKDAG KKAGPGIQKT RTGRLKKPFL KVEDVNRCYR PFYLQLPSLP CINYFLQKPC SPFDIEKSSS VQKQAQPKLR INMDGDKCGT PVQLQLKEKR KKGYCECCLQ KYEDLETHLL SEKHRNFAQS NQYQVVDDIV SQLVFDFVEY GRDTPQKKRI RYSVGSLSSV SANVLKNTAP KEKPLLEPNF QKDVGESSGH LLKPNSQYEE TQKPEEKHGF ASEPTTYSSA GLKGCDRKPV SMFNASEPDP EQEYAQLPLR DSTPEHQVTE GRNDGEQRVD PAPGVSQSCG QVSHLSTESN LPQPQLAADI TQLSAKDLQE KGFHVVIGHA SDLVALNTSK EQLTMKARTP PCSPQEPHEC DTENMENLPC GKIQRKVRML LGQQKANAEP SAELDKKRTE YLPAHEDRTC GSPVQSLLDL FQTSEEKSEF LGFTGYTENS GICDVLDIWE EENSSTLLST FFSSPSTSAF VGF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role DNA in replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Subcellular Location
Nucleus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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