Recombinant Mouse Protein DEK (Dek)

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Code CSB-BP766504MO
Size $528
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Dek
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
DekProtein DEK
Species
Mus musculus (Mouse)
Source
Baculovirus
Expression Region
1-380aa
Target Protein Sequence
MSAAAAPAAEGEDAPVPPSSEKEPEMPGPREESEEEEEDDEDDDEEDEEEEKEKSLIVEGKREKKKVERLTMQVSSLQREPFTVTQGKGQKLCEIERIHFFLSKKKPDELRNLHKLLYNRPGTVSSLKKNVGQFSGFPFEKGSTQYKKKEEMLKKFRNAMLKSICEVLDLERSGVNSELVKRILNFLMHPKPSGKPLPKSKKSSSKGSKKERNSSGTTRKSKQTKCPEILSDESSSDEDEKKNKEESSEDEEKESEEEQPPKKTSKKEKAKQKATAKSKKSVKSANVKKADSSTTKKNQKSSKKESESEDSSDDEPLIKKLKKPPTDEELKETVKKLLADANLEEVTMKQICKEVYENYPAYDLTERKDFIKTTVKELIS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
45.7 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The gene responsible for the Mouse Dek protein (1-380aa) is cloned into a plasmid vector to obtain recombinant plasmid, which is then transformed into baculovirus cells. baculovirus cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to endure a specific antibiotic. The baculovirus cells containing the recombinant plasmid are cultured under conditions promoting the expression of the gene of interest. The protein carries a N-terminal 10xHis tag. Post-expression, affinity purification is employed to isolate and purify the recombinant Mouse Dek protein from the cell lysate. The resulting recombinant protein is subjected to denaturing SDS-PAGE, allowing for an estimation of its purity, surpassing 85%.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
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Applications : Western blot analysis

Review: Western blot analysis showed a significant reduction of DEK protein in the DTA6-treated RAW264.7 cells group. Above: in each group measured by Western blot analysis; Below: expression of DEK protein in RAW264.7 cells and relative intensity. Data are presented as mean ± SD.

By Anonymous

Target Background

Function
Involved in chromatin organization.
Gene References into Functions
  1. These findings define DEK as an important and multifunctional mediator of homologous recombination. PMID: 28317934
  2. DEK is crucial to the development of arthritis in mouse models, thus making it an appropriate target for aptamer-based therapy. PMID: 28165452
  3. DEK was expressed throughout the brain in both sexes, including the medial prefrontal cortex (prelimbic, infralimbic and dorsal peduncular). DEK was also abundant in all amygdalar subdivisions (basolateral, central and medial) and in the hippocampus including the CA1, CA2, CA3, dentate gyrus (DG), ventral subiculum and entorhinal cortex. PMID: 29175155
  4. Dek contributes to esophageal squamous cell carcinoma tumorigenesis at least partially through keratinocyte intrinsic pathways which promote cellular and tumor growth. PMID: 29538372
  5. Data suggests that DEK participates in the process of embryo implantation and might play a role in influencing stromal cell decidualization through mediating cell proliferation, apoptosis and DNA damage. PMID: 29109007
  6. DEK expression during normal hematopoiesis did reveal a relationship with specific cell types implicating a distinct function during myeloid differentiation PMID: 25128083
  7. Studies demonstrate that DEK overexpression, due in part to Ron receptor activation, drives breast cancer progression through the induction of Wnt/beta-catenin signaling. PMID: 24954505
  8. Results highlight a novel function of DEK as a guardian of chromatin by restricting widespread histone access, and in particular by modulating differential H3.3 loading in specific chromatin areas. PMID: 25049225
  9. data demonstrate that DEK stimulates HNSCC cellular growth and identify DeltaNp63 as a novel DEK effector PMID: 24608431
  10. DEK has potent effects on HSCs, HPCs, and hematopoiesis, information of biological and potential clinical interest. PMID: 21943234
  11. The hallmark of translocation in acutte myeloid leukemiaa is the expression of the DEK/CAN fusion protein. PMID: 20827285
  12. DEK is a neuroblastoma tumor antigen. PMID: 17397536
  13. Our conclusion is that DEK could serve as an architectural protein at the promoter or enhancer sites of a subfraction of human genes. PMID: 17524367
  14. Overexpression of the cellular DEK protein promotes epithelial transformation in vitro and in vivo. PMID: 19223548

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Subcellular Location
Nucleus.
Database Links
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