Recombinant Mouse Protein phosphatase 1 regulatory subunit 3B (Ppp1r3b)

Code CSB-YP804922MO
MSDS
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Source Yeast
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Code CSB-EP804922MO
MSDS
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Source E.coli
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Code CSB-EP804922MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP804922MO
MSDS
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Source Baculovirus
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Code CSB-MP804922MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Ppp1r3b; Ppp1r4; Protein phosphatase 1 regulatory subunit 3B; Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL; Protein phosphatase 1 regulatory subunit 4; PP1 subunit R4; Protein phosphatase 1 subunit GL; PTG
Species
Mus musculus (Mouse)
Expression Region
1-284
Target Protein Sequence
MAVDIQYSYS SMAPSLRRER FTFKISPKLS KPLRPCIQLG SKDEASGMVA PAVQEKKVKK RVSFADNQGL ALTMVKVFSE FDDPLDIPFN ITELLDNIVS LTTAESESFV LDFPQPSADY LDFRNRLQTN HVCLENCVLK DKAIAGTVKV QNLAFEKVVK IRMTFDTWKS FTDFPCQYVK DTYAGSDRDT FSFDISLPEK IQSYERMEFA VCYECNGQAY WDSNKGKNYR ITRAELRSSP GKIEPYNGPD FGISFDQFGS PRCSFGLFPE WPSYLGYEKL GPYY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes.
Gene References into Functions
  1. Mice lacking protein phosphatase 1 regulatory subunit 3B (PPP1R3B) were deficient in hepatic glycogen, whereas HTGC was unchanged. Hepatic overexpression of PPP1R3B caused accumulation of hepatic glycogen and elevated plasma levels of ALT, but did not change HTGC PMID: 29266543
  2. findings indicate a major role for Ppp1r3b in regulating hepatic glycogen stores and whole-body glucose/energy homeostasis. PMID: 28473467
  3. analysis of expression of alternative splice variants of the mouse Ppp1r3b gene in lung epithelial cells PMID: 16949035
Tissue Specificity
Highly expressed in liver (at protein level). Expressed predominantly in liver. Expressed moderately in heart. Expressed weakly in prostate, stomach, thyroid, lung, kidney, spleen and skeletal muscle.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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