Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
Pdxp; Cin; Plp; Plpp; Pyridoxal phosphate phosphatase; PLP phosphatase; EC 3.1.3.3; EC 3.1.3.74; Chronophin
Species
Mus musculus (Mouse)
Expression Region
1-292aa
Target Protein Sequence
MARCERLRGAALRDVLGQAQGVLFDCDGVLWNGERIVPGAPELLQRLARAGKNTLFVSNNSRRARPELALRFARLGFAGLRAEQLFSSALCAARLLRQRLSGPPDASGAVFVLGGEGLRAELRAAGLRLAGDPGEDPRVRAVLVGYDEQFSFSRLTEACAHLRDPDCLLVATDRDPWHPLSDGSRTPGTGSLAAAVETASGRQALVVGKPSPYMFQCITEDFSVDPARTLMVGDRLETDILFGHRCGMTTVLTLTGVSSLEEAQAYLTAGQRDLVPHYYVESIADLMEGLED
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This Mouse Pdxp recombinant protein was produced in E.coli, where the gene sequence encoding Mouse Pdxp (1-292aa) was expressed with the N-terminal 6xHis-SUMO tag. The purity of this Pdxp protein was greater than 90% by SDS-PAGE.
Pdxp is a phosphatase enzyme with a primary function of removing phosphate groups from vitamin B6, converting pyridoxal phosphate (PLP) to pyridoxal (PL). This reaction is a crucial step in the metabolism of vitamin B6. Vitamin B6 and its active form, PLP, are essential for maintaining various physiological functions, including amino acid metabolism, neurotransmission, and hemoglobin synthesis. Pdxp, by regulating PLP levels, is involved in these important biochemical pathways. Vitamin B6 deficiency can lead to various health problems, including anemia, neurological issues, and immune system disorders. Therefore, understanding vitamin B6 metabolism and studying enzymes like Pdxp holds potential significance for the treatment of these diseases.