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Recombinant Mouse Pyridoxal phosphate phosphatase (Pdxp)

Recombinant Mouse Pyridoxal phosphate phosphatase (Pdxp)

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Code	CSB-EP017749MO
Abbreviation	Recombinant Mouse Pdxp protein
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP017749MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Pdxp. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP017749MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Pdxp.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Pdxp

Uniprot No.

P60487

Research Area

Signal Transduction

Alternative Names

Pdxp; Cin; Plp; Plpp; Pyridoxal phosphate phosphatase; PLP phosphatase; EC 3.1.3.3; EC 3.1.3.74; Chronophin

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-292aa

Target Protein Sequence

MARCERLRGAALRDVLGQAQGVLFDCDGVLWNGERIVPGAPELLQRLARAGKNTLFVSNNSRRARPELALRFARLGFAGLRAEQLFSSALCAARLLRQRLSGPPDASGAVFVLGGEGLRAELRAAGLRLAGDPGEDPRVRAVLVGYDEQFSFSRLTEACAHLRDPDCLLVATDRDPWHPLSDGSRTPGTGSLAAAVETASGRQALVVGKPSPYMFQCITEDFSVDPARTLMVGDRLETDILFGHRCGMTTVLTLTGVSSLEEAQAYLTAGQRDLVPHYYVESIADLMEGLED
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

47.5kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Mouse Pdxp recombinant protein was produced in E.coli, where the gene sequence encoding Mouse Pdxp (1-292aa) was expressed with the N-terminal 6xHis-SUMO tag. The purity of this Pdxp protein was greater than 90% by SDS-PAGE.
Pdxp is a phosphatase enzyme with a primary function of removing phosphate groups from vitamin B6, converting pyridoxal phosphate (PLP) to pyridoxal (PL). This reaction is a crucial step in the metabolism of vitamin B6. Vitamin B6 and its active form, PLP, are essential for maintaining various physiological functions, including amino acid metabolism, neurotransmission, and hemoglobin synthesis. Pdxp, by regulating PLP levels, is involved in these important biochemical pathways. Vitamin B6 deficiency can lead to various health problems, including anemia, neurological issues, and immune system disorders. Therefore, understanding vitamin B6 metabolism and studying enzymes like Pdxp holds potential significance for the treatment of these diseases.

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Target Background

Function

Functions as a pyridoxal phosphate (PLP) phosphatase, which also catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), with order of substrate preference PLP > PNP > PMP and therefore plays a role in vitamin B6 metabolism. Also functions as a protein serine phosphatase that specifically dephosphorylates 'Ser-3' in proteins of the actin-depolymerizing factor (ADF)/cofilin family like CFL1 and DSTN. Thereby, regulates cofilin-dependent actin cytoskeleton reorganization, being required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phosphothreonines in LIMK1. Does not dephosphorylate peptides containing phosphotyrosine.

Gene References into Functions

Results provided novel evidence that PLPP is involved in controlling dendritic spine as well as synaptic plasticity. It showed also for the first time the role of PLPP in GluN functionality via regulation of GluN2A interaction with postsynaptic proteins. These findings suggest that PLPP/CIN may play an important role in information storage and recall capacity, which manifests as a learning memory. PMID: 27212638
Evolutionary and structural analyses of mammalian haloacid dehalogenase-type phosphatases AUM and chronophin provide insight into the basis of their different substrate specificities. PMID: 24338473
Dimer formation is essential for an intermolecular arginine-arginine-tryptophan stacking interaction that positions a critical histidine residue in the substrate specificity loop of chronophin for PLP coordination. PMID: 24338687
analysis of pyridoxal phosphatase cloning, expression and tissue distribution PMID: 14522954

Subcellular Location

Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

HAD-like hydrolase superfamily

Tissue Specificity

Ubiquitous. highly expressed in brain (at protein level).

Database Links

KEGG: mmu:57028

STRING: 10090.ENSMUSP00000086796

UniGene: Mm.263169

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