Recombinant Mouse Rab11 family-interacting protein 5 (Rab11fip5)

Code CSB-YP837136MO
MSDS
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Source Yeast
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Code CSB-EP837136MO
MSDS
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Source E.coli
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Code CSB-EP837136MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP837136MO
MSDS
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Source Baculovirus
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Code CSB-MP837136MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rab11fip5
Uniprot No.
Alternative Names
Rab11fip5; D6Ertd32e; Rip11; Rab11 family-interacting protein 5; Rab11-FIP5; Rab11-interacting protein Rip11
Species
Mus musculus (Mouse)
Expression Region
1-645
Target Protein Sequence
MALVRDPEPA AGSSRWLPTH VQVTVLRASG LRGKSSGAGS TSDAYTVIQV GREKYSTSVV EKTQGCPEWC EECSFELPPG ALDGLLRAQE ADAGPAPWAS GPNAACELVL TTMHRSLIGV DKFLGRATVA LDEVFRAGRA QHTQWYRLHS KPGKKEKERG EIQVTIQFTR NNLSASMFDL SMKDKPRSPF SKLKDRVKGK KKYDLESASA ILPSSALEDP ELGSLGKMGK AKGFFLRNKL RKSSLTQSNT SLGSDSTLSS TSGSLVYQGP GAELLTRSPS HSSWLSTEGG RDSIQSPKLL THKRTYSDEA SQLRAAPPRA LLELQGHLDG ASRSSLCVNG SHVYNEEPQP PLRHRSSISG PFPPSSSLHS VPPRSSEEGS RSSDDSWGRG SHGTSSSEAV PGQEELSKQA KGASCSGEEE GARLPEGKPV QVATPMVASS EAVAAEKDRK PRMGLFHHHH HQGLSRSEQG RRGSVGEKGS PSLGASPHHS STGEEKAKSS WFGLRESKEP TQKPSPHPVK PLTAAPVEAS PDRKQPRTSL STALSSGLER LKTVTSGGIQ SVLPASQLGS SVDTKRPKDS AVLDQSAKYY HLTHDELIGL LLQRERELSQ RDEHVQELES YIDRLLVRIM ETSPTLLQIS PGPPK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane. Involved in insulin granule exocytosis. May regulate V-ATPase intracellular transport in response to extracellular acidosis.
Gene References into Functions
  1. the ability of insulin to increase the appearance of Rip11 at the cell surface involves an inhibition of Rip11 internalisation from the plasma membrane PMID: 26515129
  2. The results of this study suggest a requirement for Rab11Fip5, and presumably Rab11, during Hippocampal Long-Term Depression. PMID: 25972173
  3. Delivery of Rab11-containing membrane stores effects broad and functional pseudopods during FcgammaR-mediated phagocytosis in mutant cultures of kinesin 5B-expressing cells. PMID: 21149599
  4. Rip11 is a Rab11- and AS160-RabGAP-binding protein required for insulin-stimulatd glucose uptake in adipocytes. PMID: 18003705
  5. Study shows that both Rab11 and its effector Rip11 participate in insulin granule exocytosis and that Rip11, as a substrate of PKA, regulates the potentiation of exocytosis by cAMP in pancreatic beta-cells. PMID: 19335615

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Subcellular Location
Cytoplasm. Recycling endosome membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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