Product Details

Purity

>85% (SDS-PAGE)

Target Names

Rab3a

Uniprot No.

P63011

Alternative Names

Rab3a; Ras-related protein Rab-3A

Species

Mus musculus (Mouse)

Expression Region

1-220

Target Protein Sequence

MASATDSRYG QKESSDQNFD YMFKILIIGN SSVGKTSFLF RYADDSFTPA FVSTVGIDFK VKTIYRNDKR IKLQIWDTAG QERYRTITTA YYRGAMGFIL MYDITNEESF NAVQDWSTQI KTYSWDNAQV LLVGNKCDME DERVVSSERG RQLADHLGFE FFEASAKDNI NVKQTFERLV DVICEKMSES LDTADPAVTG AKQGPQLTDQ QAPPHQDCAC

Protein Length

Full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 isoform RIMS2 and RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT.

Gene References into Functions

Rab3A might function as a marker of cortical granules and participates in cortical granule exocytosis in mouse eggs. PMID: 27423421
Rab3a accelerates cell proliferation by increasing cyclin D1 expression, enhances anti-cancer drug resistance, and increases tumorigenicity and self-renewal of glioma cells. PMID: 24965146
Rab3a deletion restores excitatory/inhibitory imbalance in synapsin II(-) hippocampal slices. PMID: 24227741
betaARs couple to a cAMP/Epac/PLC/Munc13-1/Rab3a/RIM1a-dependent pathway to enhance glutamate release at cerebrocortical nerve terminals. PMID: 24036110
key role of Rab3a proteins control the balance between secretory vesicle biogenesis and degradation, and suggest that the ablation of this protein probably changes the nature of the reservoir of secretory vesicles available for regulated exocytosis. PMID: 23399968
alpha-Synuclein membrane association is regulated by the Rab3a recycling machinery and presynaptic activity PMID: 23344955
Data show diminished actin plasticity in the cell processes of Rab3A knockout (KO) podocytes. PMID: 22472623
these findings suggest a crucial role of Rab3A in delivering vesicles to Ca2+ -dependent release sites at ribbon synapses. PMID: 22593061
We conclude that Rab3A is involved in a novel presynaptic mechanism to homeostatically regulate the amount of transmitter in a quantum. PMID: 21389214
roles in maintaining the total store of synaptic vesicles and cooperate in promoting the latest steps of neuronal secretion. PMID: 20338242
We suggest that Rab3A may regulate SNAP-29-mediated membrane fusion during myelination PMID: 19170188
Data show that Rab3A is highly and specifically expressed in brain regions. PMID: 19570198
The monomeric GTP binding protein, rab3a, is associated with the acrosome in mouse sperm. PMID: 10398417
role in regulatory mechanism of neurotransmitter release PMID: 11871104
Phenotypic assessment of earlybird mice and a null allele of Rab3a revealed anomalies in circadian period and sleep homeostasis, providing evidence that Rab3a-mediated synaptic transmission is involved in these behaviors PMID: 12244319
Rab3A protein has a role in exocytosis PMID: 12438138
Rab3A reduces the potency of adenosine as an endogenous mediator of neuromuscular depression PMID: 12456815
Rab3A plays an important in vivo role facilitating the efficiency of insulin exocytosis, most likely at the level of replenishing the ready releasable pool of beta-granules PMID: 12510060
a possible role in glomerulopathies PMID: 12937130
Mice deficient for the synaptic vesicle protein Rab3a show impaired spatial reversal learning and increased explorative activity. PMID: 15078563
Rab3A and its effectors are general modules for four distinct types of PKA-dependent LTP in the brain PMID: 15967982
Rab3-induced modifications to primed vesicles causes a transient increase in the transduction efficacy of synaptic action potential trains and optimizes the encoding of synaptic information at an intermediate spike frequency range. PMID: 16436611
Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2 PMID: 16704978
Data implicate Rab3A in learning and memory as well as in the regulation of emotion. Differences in genetic background have a stronger effect on gene expression than the mutations in the Rab3a gene. PMID: 16734774
In p130-deficient mice the GTP-bound form of Rab3A accumulated in the brain. PMID: 16782817
Rab3a positively regulates docking and basal quantal release at the mouse neuromuscular junction. This result is consistent with the proposed role of rab3a in trafficking and targeting vesicles to the active zones. PMID: 17640821
Rab3GEP has a role as the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes PMID: 18559336
We found that small amplitude pre-spike feet with abnormally long durations were increased in Rab3A(-/-) cells. PMID: 18591190
Results support the hypothesis that Rab3a maintains the pool of fusion competent vesicles tightly coupled to Ca2+ channels. PMID: 19348946

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Subcellular Location

Cytoplasm, cytosol. Lysosome. Cytoplasmic vesicle, secretory vesicle. Cell projection, axon. Cell membrane; Lipid-anchor; Cytoplasmic side.

Protein Families

Small GTPase superfamily, Rab family

Tissue Specificity

Found in active zone in brain nerve terminals.

Database Links

KEGG: mmu:19339

STRING: 10090.ENSMUSP00000034301

UniGene: Mm.5083

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Recombinant Mouse Ras-related protein Rab-3A (Rab3a)

Product Details

Customer Reviews and Q&A

Target Background

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