Recombinant Mouse Retinoic acid receptor alpha(Rara)

Code CSB-YP019338MO
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Source Yeast
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Code CSB-EP019338MO
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Source E.coli
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Code CSB-EP019338MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019338MO
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Source Baculovirus
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Code CSB-MP019338MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Rara
Uniprot No. P11416
Alternative Names Rara; Nr1b1; Retinoic acid receptor alpha; RAR-alpha; Nuclear receptor subfamily 1 group B member 1
Species Mus musculus (Mouse)
Expression Region 1-462
Target Protein Sequence MASNSSSCPT PGGGHLNGYP VPPYAFFFPP MLGGLSPPGA LTSLQHQLPV SGYSTPSPAT IETQSSSSEE IVPSPPSPPP LPRIYKPCFV CQDKSSGYHY GVSACEGCKG FFRRSIQKNM VYTCHRDKNC IINKVTRNRC QYCRLQKCFD VGMSKESVRN DRNKKKKEAP KPECSESYTL TPEVGELIEK VRKAHQETFP ALCQLGKYTT NNSSEQRVSL DIDLWDKFSE LSTKCIIKTV EFAKQLPGFT TLTIADQITL LKAACLDILI LRICTRYTPE QDTMTFSDGL TLNRTQMHNA GFGPLTDLVF AFANQLLPLE MDDAETGLLS AICLICGDRQ DLEQPDKVDM LQEPLLEALK VYVRKRRPSR PHMFPKMLMK ITDLRSISAK GAERVITLKM EIPGSMPPLI QEMLENSEGL DTLSGQSGGG TRDGGGLAPP PGSCSPSLSP SSHRSSPATQ SP
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone deacetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. Formation of heterocomplex with histone deacetylases might lead to inhibition of RARE DNA element binding and to transcriptional repression. Transcriptional activation and RARE DNA element binding might be supported by the transcription factor KLF2. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. Together with RXRA, positively regulates microRNA-10a expression, thereby inhibiting the GATA6/VCAM1 signaling response to pulsatile shear stress in vascular endothelial cells. In association with HDAC3, HDAC5 and HDAC7 corepressors, plays a role in the repression of microRNA-10a and thereby promotes the inflammatory response.
Gene References into Functions
  1. these findings indicate that epithelial cell-intrinsic RARalpha signaling is critical to the global development of the intestinal immune system PMID: 29139475
  2. This study demonstrates that RA signaling is required in steroidogenic cells for their normal function and, thus, for male fertility. PMID: 29899137
  3. RARalpha and RARgamma reciprocally control K5(+) progenitor cell proliferation and distribution in the developing submandibular salivary epithelium in a cell cycle-dependent manner while regulating lumenization independently of keratinizing differentiation PMID: 28933645
  4. These data suggest that impairment of cardiac RARalpha signaling may be a novel mechanism that is directly linked to pathological stimuli-induced diastolic dysfunction. PMID: 27539860
  5. Retinoic acid receptor alpha agonist all trans retinoic acid can protect the liver from ischemia reperfusion injury by promoting autophagy, which is dependent on Foxo3/p-Akt/Foxo1 signaling. PMID: 26604645
  6. the reprogramming of epiblast stem cells into embryonic stem cell-like cells also requires low levels of retinoic acid (RA), which can modulate Wnt signalling through physical interactions of RARs with beta-catenin. PMID: 25546009
  7. although X-RARA fusion proteins have been suggested to act by blocking retinoid-dependent transcriptional programs , we observed a surprising paucity of natural retinoids capable of transactivating Gal4-RARA in primary mouse bone marrow cells PMID: 25723855
  8. The results indicate a physiological role for RARgamma as a negative regulator of osteoclastogenesis in vivo and in vitro, and reveal distinct influences of RARalpha and RARgamma in bone structure regulation. PMID: 25800721
  9. Retinoic acid-RARalpha as a key component of the regulatory network governing maintenance and plasticity of Th1-cell fate. PMID: 25769610
  10. atRA signaling mediated by RARa is required in the adult pancreas for maintaining both beta-cell function and mass PMID: 25389133
  11. A regulatory network, incluing Sox17 and the retinoic acid receptor, controls nephrocan expression and midgut patterning. PMID: 25209250
  12. Report liver RAR-alpha mediated response to retinoic acid. PMID: 24833708
  13. Rbp4 and its membrane receptor STRA6 control adipogenesis by regulating cellular retinoid homeostasis and RARalpha activity. PMID: 23959802
  14. RARalpha2 has a role in determining myeloma stem cell features in mouse and human cells PMID: 23847194
  15. CTBP2 is a transcriptional cofactor for RXR-alpha/RAR-alpha. PMID: 23775127
  16. Uncoupling RARA transcriptional activation and degradation clarifies the bases for APL response to therapies. PMID: 23509325
  17. analysis of retinoic acid receptor-dependent, cell-autonomous, endogenous retinoic acid signaling and its target genes in mouse collecting duct cells PMID: 23049847
  18. Results define PARG as a coactivator regulating chromatin remodeling during retinoic acid receptor-dependent gene expression. PMID: 23102699
  19. in vitamin A-replete mice, RARalpha1 is required to maintain normal mammary morphogenesis, but paradoxically, also efficient tumorigenesis PMID: 20923554
  20. Retinoic acid receptor and CNGA2 channel signaling are part of a regulatory feedback loop controlling axonal convergence and survival of olfactory sensory neurons. PMID: 22009938
  21. RARalpha and RARgamma are present in pluripotent and differentiating mEC and mES cells and suggest that the expression of these proteins is dynamic. PMID: 21987218
  22. These results identify Trim24 as a novel negative regulator of the IFN/STAT pathway and suggest that this repression through Rara inhibition may prevent liver cancer. PMID: 21768647
  23. RAR-a, RXR-a and TF may emerge as important regulatory molecules and play important role in liver injury. PMID: 21658367
  24. Report that Am580, a water-soluble RARalpha-specific agonist, attenuated proteinuria, glomerosclerosis, and podocyte proliferation, and restored podocyte differentiation markers in kidneys of HIV-associated nephropathy mice. PMID: 21150871
  25. reveal a fundamental role for the RA-RARalpha axis in the development of both regulatory and inflammatory arms of adaptive immunity and establish nutritional status as a broad regulator of adaptive T cell responses PMID: 21419664
  26. CART1 might be a cytoplasmic, testis-specific derepressor of RAR PMID: 20736163
  27. The specific defects in spermiogenesis in RARalpha-deficient testes may correlate with a disrupted cyclic expression of retinoic acid-responsive structural components, including vimentin, connexin-40 and ZO-1. PMID: 19937743
  28. Retinoic acid-suppressed phosphorylation of RARalpha induces FGF8f expression to mediate differentiation response pathway in U2OS osteosarcoma cells. PMID: 20190807
  29. Protein Kinase C alpha can influence retinoic acid signaling by altering the stability of Retinoic Acid Receptor alpha protein without directly phosphorylating this receptor. PMID: 12000751
  30. RA-induced keratinocyte proliferation was studied in mutant mice in which RXR alpha, RXR alpha and RAR alpha, RAR gamma, or RXR alpha and RAR gamma genes were specifically disrupted in either basal or suprabasal keratinocytes. PMID: 12093741
  31. the number, migration, and terminal fate of the cardiac neural crest is normal in mutant embryos; however, the specific function of these cells in forming the aorticopulmonary septum is impaired PMID: 12204252
  32. RARalpha and RARgamma play an essential role in the initial differentiation of otic placode derivatives, whereas RARbeta plays a minimal role in this process PMID: 12464434
  33. regulates pulmonary alveolus formation after, but not during, perinatal period PMID: 12533315
  34. signals mediated through retinoic acid receptor alpha (RAR alpha), but not RAR beta or gamma, required both Mad1 and p27(Kip1) to induce cell cycle arrest and to accelerate terminal differentiation of granulocytes PMID: 14576045
  35. RARalpha and RXRalpha play crucial roles in organogenesis and in the growth and development of murine embryos PMID: 14967906
  36. acute promyelocytic leukemia cell differentiation parallels transcriptional activation through PML-RARA-RXR oligomers PMID: 15096541
  37. crystallization of the heterodimeric complex of RARbeta and RXRalpha ligand-binding domains in the active conformation PMID: 15159591
  38. RARalpha has a role in retinoid signaling and synchronous progression of spermatogenesis PMID: 15254909
  39. RARs are not essential for p63 expression PMID: 15528198
  40. Expression profiles of normal promyelocyte-enriched samples and nontransformed promyelocytes expressing RARalpha1 were similar to the wild type promyelocytes. However, transformed cells from acute promyelocytic leukemia systems were all different. PMID: 15572690
  41. Retinoic acid modulates the expression of RARalpha in brown adipose tissue, suggesting auto regulation of the retinoid effect on the thermogenic system. PMID: 15646024
  42. RARalpha, beta, and gamma isotypes each have specific and distinct roles during the initial stages of neural differentiation of PCC7 cells PMID: 15831518
  43. Sterility in RARalpha(-/-) males is associated with specific defects in spermiogenesis, which may correlate with a failure in both spermatid release and spermatid orientation. PMID: 15901285
  44. These results demonstrate that the dimerization interface of RARalpha fusion partners is a critical element in APL pathogenesis while pointing to other features of PML for enhancing penetrance and progression. PMID: 16473276
  45. Taken together, these findings endorse an oligomerization-dependent mechanism for RARalpha-mediated transformation and suggest a potential avenue for molecular therapy. PMID: 16473277
  46. physiological RAR activity may normally suppress B lymphocyte and mammary epithelial cell growth and that global RAR inactivation is sufficient to initiate a stochastic process of tumor development requiring multiple transforming events PMID: 16563162
  47. prevalence of anti-RARalpha antibodies in acute promyelocytic leukemia PMID: 16728697
  48. The ablation of all RXR (alpha, beta and gamma isotypes) in Sertoli cells does not recapitulate the phenotype generated upon ablation of all three retinoic acid receptors. PMID: 17124491
  49. identified a trimethylation site on Lys(347) in the ligand binding domain PMID: 17205979
  50. Two highly related subunits of the core SWI/SNF complex, BAF60c1 and BAF60c2, interact physically with retinoid receptors and are coactivators for retinoic acid receptors. PMID: 17363140

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Subcellular Location Nucleus. Cytoplasm.
Protein Families Nuclear hormone receptor family, NR1 subfamily
Tissue Specificity Expressed in Sertoli cells and germ cells.
Database Links

KEGG: mmu:19401

STRING: 10090.ENSMUSP00000069744

UniGene: Mm.439744

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