Recombinant Mouse Semaphorin-4C (Sema4c), partial

Code CSB-YP723720MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP723720MO
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP723720MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP723720MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP723720MO
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Sema4c
Uniprot No.
Alternative Names
Sema4c; S4c; Semacl1; Semai; Semaphorin-4C; M-Sema F; Semaphorin-C-like 1; Sema I; Semaphorin I
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

What are the price of all size of the product CSB-MP723720MO in the version 6xhis tagged + My c tagged? Also I would like to know what is the protein sequence of these product? Is there any negative control available for these product?

A:
Thanks for your inquiry.
Express Region:21-500aa; Partial, contain Sema Domain
Tag information:EP,BP,MP:N-terminal 10xHis-tagged and C-terminal Myc-tagged;YP:N-terminal 6xHis-tagged
Express Sequence:

AEMWWNLVPRKTVSSGELVTVVRRFSQTGIQDFLTLTLTEHSGLLYVGAREALFAFSVEALELQGAISWEAPAEKKIECTQKGKSNQTECFNFIRFLQPYNSSHLYVCGTYAFQPKCTYINMLTFTLDRAEFEDGKGKCPYDPAKGHTGLLVDGELYSATLNNFLGTEPVILRYMGTHHSIKTEYLAFWLNEPHFVGSAFVPESVGSFTGDDDKIYFFFSERAVEYDCYSEQVVARVARVCKGDMGGARTLQKKWTTFLKARLVCSAPDWKVYFNQLKAVHTLRGASWHNTTFFGVFQARWGDMDLSAVCEYQLEQIQQVFEGPYKEYSEQAQKWARYTDPVPSPRPGSCINNWHRDNGYTSSLELPDNTLNFIKKHPLMEDQVKPRLGRPLLVKKNTNFTHVVADRVPGLDGATYTVLFIGTGDGWLLKAVSLGPWIHMVEELQVFDQEPVESLVLSQSKKVLFAGSRSQLVQLSLADC


As to the negative control, can you let me know which experiment will you use this protein? Usually we could use the protein's buffer as negative control.

Target Background

Function
Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.
Gene References into Functions
  1. Follicular T helper cells use Semaphorin 4C as a guidance receptor to sense germinal center-expressed Plexin B2. PMID: 28467912
  2. we demonstrate that Sema4C is critical for optimal regulatory cytokine production in CD138(+) B cells. PMID: 27881703
  3. Sema4C-Plexin B2 signalling regulates ureteric branching PMID: 21035938
  4. We have identified here Sema4C and Sema4G as candidate ligands for Plexin-B2 PMID: 21122816
  5. Plexin-B2 and Sema4C are potential regulators of the vascular and endocrine system. PMID: 20478304
  6. Results suggest a putative role of Sema4C during neurogenesis both in vivo and in vitro. PMID: 19189244
  7. Sema4C-mediated interaction among myoblasts plays an important role in terminal myogenic differentiation PMID: 15811348
  8. Sema4C promotes terminal myogenic differentiation in a p38 MAPK-dependent manner. PMID: 17498836

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Subcellular Location
Cell junction, synapse, postsynaptic density membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type I membrane protein.
Protein Families
Semaphorin family
Tissue Specificity
Predominantly expressed in brain (at protein level).
Database Links
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