Recombinant Mouse Solute carrier family 2, facilitated glucose transporter member 4(Slc2a4),partial

Code CSB-YP021557MO
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Source Yeast
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Code CSB-EP021557MO
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Source E.coli
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Code CSB-EP021557MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021557MO
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Source Baculovirus
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Code CSB-MP021557MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Slc2a4
Uniprot No. P14142
Alternative Names Slc2a4; Glut4; Solute carrier family 2; facilitated glucose transporter member 4; GT2; Glucose transporter type 4; insulin-responsive; GLUT-4
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Insulin-regulated facilitative glucose transporter.
Gene References into Functions
  1. Those findings provide new insight into the mechanisms responsible for AMPKalpha2-dependent regulation of GLUT4 transcription after exercise. PMID: 28688716
  2. Portulaca oleracea L. extract enhanced glucose uptake, which was caused by increased GLUT4 expression at the plasma membrane through activating the PI3K/Akt pathway. PMID: 29341802
  3. 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine retains cell surface GLUT4 by suppressing PKC alpha-driven endocytic internalization of GLUT4, to enhance glucose uptake into cells and restrict an increase in the blood glucose levels after glucose loading in type 2 Diabetes Mellitus. PMID: 29723855
  4. The data, therefore, suggest that 1,25(OH)2D3 increases glucose consumption by inducing SIRT1 activation, which in turn increases IRS1 phosphorylation and GLUT4 translocation in myotubes. PMID: 29188534
  5. Cell-autonomous adiposity results from increased cell surface GLUT4 due to ankyrin-B deficiency in humans and mice. PMID: 29133412
  6. G4+/- offspring on a High Fat Diet displayed early hypertension associated with increased renal gene expression of renin and the AT1- receptors compared to G4+/- on a C diet. This group showed decreased cardiac expression of key genes involved in fatty acid oxidation compared to WT on a C diet. PMID: 28750406
  7. dynamin is a molecular motor which would be involved in GLUT4 translocation by facilitating exocytosis PMID: 28853753
  8. Insulin-stimulated translocation of GLUT4 and GLUT8 was down-regulated in the atria of insulin resistance animals, as well as their total protein expression. PMID: 29291943
  9. The disruption of SM22alpha enhances PDGF-BB-induced GLUT4 translocation and glucose uptake by promoting actin dynamics and cortical actin polymeriza- tion. PMID: 27631639
  10. Data suggest that Glut4 expression is glucose-dependent in white adipocytes; low glucose availability reduces total NADH/NADPH levels; high glucose availability up-regulates total NADH/NADPH levels, promotes adipogenesis and lipogenesis, and induces Glut4 expression. PMID: 28916720
  11. Glucose transporter 4 (GLUT4) is required for myocardial adaptations to exercise, and its absence accelerates heart dysfunction after pressure overload. PMID: 28822962
  12. Data show that TBK1 directly interacts with Exo84 through the coiled-coil domain of TBK1 and helical domain of Exo84, and knockdown of TBK1 blocked insulin-stimulated glucose uptake and GLUT4 translocation. PMID: 28325821
  13. E4-ORF1 activation of PI3K in adipocytes recapitulates insulin regulation of FoxO1 but not regulation of Glut4. This uncoupling of PI3K effects occurs despite E4-ORF1 activating PI3K and downstream signaling to levels achieved by insulin. PMID: 28009298
  14. Authors suggest that sortilin- and retromer-mediated Glut4 retrieval from endosomes may represent a step in the Glut4 pathway vulnerable to the development of insulin resistance and diabetes. PMID: 28450454
  15. It was concluded that ILK depletion modifies the transcription of GLUT4, which results in reduced peripheral insulin sensitivity and glucose uptake, suggesting ILK as a molecular target and a prognostic biomarker of insulin resistance. PMID: 28490443
  16. Data, including data from studies using knockout/transgenic mice, suggest that PrPC is involved in development of insulin resistance and obesity; primary embryonic fibroblasts cultured from PrPC knockout mice exhibit reduced glucose uptake upon insulin stimulation due to reduced translocation of glucose transporter Glut4 to cell membrane. (PrPC = cellular prion protein; Glut4 = facilitated glucose transporter 4) PMID: 28739602
  17. Our findings implicate Rac1 as a regulatory element critical for controlling glucose uptake during exercise via regulation of GLUT4 translocation. PMID: 27061726
  18. insulin and insulin resistance regulate the spatial organization of GLUT4 in adipocytes. PMID: 27888215
  19. these results indicate that PI3K and Akt ( Akt1-Akt3)play distinct roles, and that PI3K stimulates Akt-independent pathways that are important for GLUT4 translocation. PMID: 27076519
  20. We confirmed that procyanidins suppressed acute hyperglycemia with an oral glucose tolerance test in a dose-dependent manner.procyanidins, especially cinnamtannin A2, significantly ameliorate postprandial hyperglycemia at least in part by promoting GLUT4 translocation to the plasma membrane by activating both insulin- and AMPK-signaling pathways. PMID: 27598258
  21. DHHC7 KO mice developed hyperglycemia and glucose intolerance, thereby confirming that DHHC7 represents the principal PAT for Glut4 and that this mechanism is essential for insulin-regulated glucose homeostasis PMID: 28057756
  22. Brain GLUT4 knockout mice are glucose intolerant, insulin resistant, and have impaired glucose sensing, indicating a critical role for brain GLUT4 in sensing and responding to changes in blood glucose. PMID: 27797912
  23. Glut4 expression in the gastrocnemius muscle was lowered under short photoperiod. Access to running wheels did not alter Glut4 expression in the gastrocnemius muscle. Photoperiodic changes in Glut4 expression may be independent of physical activity. PMID: 28189688
  24. SEC16A and RAB10 promote insulin-stimulated mobilization of GLUT4 from a perinuclear recycling endosome/trans Golgi network compartment. PMID: 27354378
  25. these results indicate an indispensable role for Rab8A in insulin-regulated GLUT4 trafficking in C2C12 cells. PMID: 28079283
  26. The findings suggest that Glut4 mediates insulin-stimulated glucose uptake by mature osteoblasts/osteocytes and that the magnitude of glucose use by bone cells is sufficient to impact global glucose disposal in the mouse. PMID: 27689415
  27. study shows that increased Skeletal Muscle GLUT4 Expression in Obese Mice After Voluntary Wheel Running Exercise Is Posttranscriptional.( PMID: 27411383
  28. studies demonstrate that Elmo2 is a new regulator of insulin-dependent Glut4 membrane translocation through modulating Rac1 activity and Akt membrane compartmentalization. PMID: 27226625
  29. The results confirmed the altered expression of HFABP, a key fatty acid transport protein, and of enolase and PGK1, the key enzymes in the glycolytic process PMID: 27966485
  30. Leucine significantly increased PPARbeta/delta expression with enhanced glucose transporter 4 (GLUT4) content and glucose uptake in myotubes. PMID: 27345255
  31. Study found that knockdown of Prep1 gene expression affects adipogenic differentiation and results in significant increase in the insulin-sensitive glucose carrier Glut4 gene expression PMID: 27193720
  32. Data suggest that trans-cinnamaldehyde, a dietary flavoring agent with suspected hypoglycemic effects, stimulates mitochondrial biogenesis in muscle fibers through up-regulation of gene expression of Pgc1a (PPARgamma coactivator 1 alpha) and Glut4. PMID: 26449747
  33. CD2AP forms a complex with GGA2, a clathrin adaptor, which sorts Glut4 to GSVs, suggesting a role for CD2AP in this process. PMID: 26546360
  34. Study shows that high-intensity training was more effective for increasing GLUT4 content and glycaemia reduction in insulin-resistant mice, perhaps because of a higher metabolic demand imposed by this form of exercise. PMID: 26467261
  35. GLUT-4 and -8 are insulin-sensitive. PMID: 26720696
  36. Glut4 traffics predominantly through the specialized Rab10-dependent pathway both before and after insulin stimulation. PMID: 26527681
  37. data suggest that insulin stimulates traffic from cellugyrin-containing to sortilin-containing membranes, and that cellugyrin-containing IGVs provide an insulin-sensitive reservoir to replenish GSVs following insulin-stimulated exocytosis of GLUT4. PMID: 26071524
  38. Transcription factor MEF2 and Zac1 mediate MIF-induced GLUT4 expression through CD74-dependent AMPK activation in cardiomyocytes PMID: 26455966
  39. Tmod3 is a novel Akt2 effector that mediates insulin-induced cortical actin remodelling and subsequent GLUT4 membrane insertion PMID: 25575350
  40. TBC1D4 is dispensable for the regulation of renal Na+ and water transport, but may play a role for GLUT4-mediated basolateral glucose uptake in distal tubules. PMID: 26336159
  41. GLUT4 defects in adipose tissue are early signs of metabolic alterations in Alms1-deficient mice, a mouse model for obesity and insulin resistance. PMID: 25299671
  42. Tctex1d2 is a novel syntaxin 4 binding protein that functions as a negative regulator of GLUT4 plasma membrane translocation through inhibition of the Doc2b-syntaxin 4 interaction PMID: 26200093
  43. These results suggest that the association of Rab10-GTP with Exoc6/6b is a molecular link between insulin signaling and the exocytic machinery in GLUT4 translocation. PMID: 26299925
  44. Data (including data from mice with Tbc1d1 gene deletion) suggest Tbc1d1 controls basal intracellular GLUT4 retention in large skeletal muscles; in small muscles (extensor digitorum longus) Tbc1d1 does not regulate basal GLUT4 cell surface exposure. PMID: 26015432
  45. Data show that in healthy transgenic mice, cardiac-specific sarcoplasmic reticulum calcium ATPase SERCA1a expression increased active cell-surface glucose transporter GLUT4 and glucose uptake in the myocardium. PMID: 25615793
  46. Data indicate that 4-phenylbutyric acid (4-PBA) increases glucose transporter type 4 (GLUT4) expression through a mechanism that involves suppression of the histonedeacetylase 5 (HDAC5) pathway, but without involving endoplasmic reticulum stress. PMID: 25011668
  47. previously unrecognized physiological role of dehydroepiandrosterone as inducer of the genes PGC-1alpha and GLUT4 in myotubes PMID: 25983323
  48. studies uncover a novel mechanism under which Glut4 palmitoylation regulates Glut4 sorting to insulin responsive vesicles, thereby insulin-dependent Glut4 membrane translocation PMID: 25824042
  49. Palmitoleic acid increases glucose uptake and the GLUT4 content, in white adipocytes, in association with AMPK activation. PMID: 25528561
  50. Data suggest that insulin/insulin receptor-stimulated GLUT4 (facilitated glucose transporter member 4) translocation to plasma membrane in adipocytes may require assembly of SNARE protein complexes. [REVIEW] PMID: 25233421

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Involvement in disease Defects in Slc2a4 may be the cause of certain post-receptor defects in non-insulin-dependent diabetes mellitus (NIDDM).
Subcellular Location Cell membrane, Multi-pass membrane protein, Endomembrane system, Multi-pass membrane protein, Cytoplasm, perinuclear region
Protein Families Major facilitator superfamily, Sugar transporter (TC 2.A.1.1) family, Glucose transporter subfamily
Tissue Specificity Skeletal and cardiac muscles; brown and white fat.
Database Links

KEGG: mmu:20528

STRING: 10090.ENSMUSP00000018710

UniGene: Mm.10661

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