Recombinant Mouse Prosaposin (Psap)

1. Accumulating evidence suggests that PGRN is essential for proper lysosomal function, but the precise mechanisms involved are not known. Here, we show that PGRN facilitates neuronal uptake and lysosomal delivery of prosaposin (PSAP), the precursor of saposin peptides that are essential for lysosomal glycosphingolipid degradation. PMID: 28541286
2. Both PSAP reduction and overexpression lead to significantly elevated extracellular progranulin (PGRN) levels. Intriguingly, PSAP knockdown increases PGRN monomers, whereas PSAP overexpression increases PGRN oligomers, partly through a protein-protein interaction. PMID: 27356620
3. Data show that matrix (ECM) stiffness leads to mechano-signal transduction in mesenchymal stem cells (MSC), which promotes mammary tumor growth in part through secretion of the signaling protein prosaposin. PMID: 28972074
4. Data suggested that the abundance of Psap in sperm sample may be a sensitive endpoint to predict PCB exposure. PMID: 26045750
5. Prosaposin facilitates sortilin-independent lysosomal trafficking of progranulin. PMID: 26370502
6. This study demonistrated that Spiral ganglion degeneration and hearing loss as a consequence of satellite cell death in saposin B-deficient mice. PMID: 25698761
7. Mesotrypsin generated saposins A-D from prosaposin, and mature caspase-14 contributed to this process by activating mesotrypsinogen to mesotrypsin. Knockdown of these proteases markedly down-regulated saposin A synthesis in skin equivalent models. PMID: 24872419
8. Low levels of prosaposin and its receptors in the mouse brain suggest the participation of prosaponin in pathological changes in the brains of dystrophic mdx mice. PMID: 24244600
9. These data show functional association between GPR37, prosaposin, and GM1 ganglioside in the plasma membrane. PMID: 24371137
10. The saposin A and saposin B deficiency AB(-/-) mice develop accumulation of multiple glycosphingolipids in various organs. PMID: 23697974
11. Prosaposin is involved in the regulation of muscle differentiation of regenerated fibres PMID: 23325523
12. Saposins A and B deficiencies attenuated GalCer-beta-galactosylceramidase and GM1-beta-galactosidase functions in the degradation of lactosylceramide in the liver. PMID: 23446636
13. These data suggest that prosaposin plays an important role in the neuronal maturation processes of the vestibular sensory epithelium and the maintenance of normal vestibular system function. PMID: 22326583
14. The saposin C deficient mice backcrossed to point mutated GCase mimics the central nervous system phenotype and biochemistry of some type 3 (neuronopathic) variants of Gaucher disease. PMID: 20047948
15. These results suggest the in vivo role of saposin C in axonal membrane homeostasis, the disruption of which leads to neurodegeneration in lysosomal storage disease. PMID: 20175216
16. UGT1 aids in the folding of sequential domain-containing proteins such as prosaposin. PMID: 20498017
17. Saposin C has multiple roles in glycosphingolipid catabolism and functions in Central Nervous System independent of its role as an stabilizer of GCase. PMID: 20015957
18. Immunoblot studies indicated that prosaposin, already abundant in the brain of WT, was dramatically increased in Sap-D(-/-) PMID: 19907127
19. A saposin-like motif within the first half of the prosaposin C terminus contains the sortilin recognition site. PMID: 19934382
20. The growth promotion and anti-apoptotic activity of prosaposin is partly through the PI3K/Akt signal pathway. PMID: 20042390
21. reconstruction of the order of internal duplications that gave rise to the four saposins by using phylogenetic tools PMID: 11734895
22. The lysosomal transport of prosaposin requires the conditional interaction of its highly conserved d domain with sphingomyelin PMID: 11856752
23. prosaposin-/- mice showed seminolipid level at approximately 150% of the normal level at the terminal stage PMID: 12810822
24. data indicate that saposin C is required for acid beta-glucosidase resistance to proteolytic degradation in the cell PMID: 12813057
25. mice deficient in prosaposin, the precursor to a family of endosomal lipid transfer proteins (LTP), exhibit specific defects in CD1d-mediated antigen presentation and lack Valpha14 NKT cells PMID: 14684827
26. The trafficking of prosaposin and GM2 activator protein to the lysosome in sertoli cells is dependent on sortilin. PMID: 15236333
27. Mice which had a mutation introduced into the prosaposin gene in the saposin D domain developed progressive polyuria and ataxia and loss of cerebellar Purkinje cells. PMID: 15345707
28. Data show that there is evolutionary conservation of the prosaposin structure and alternative splicing, and in expression in the mouse and chick developing brain. PMID: 15469878
29. The prosaposin variant containing the exon 8-encoded three amino acids is dispensable for normal mouse development and fertility as well as for prosaposin secretion and its lysosomal function. PMID: 15743835
30. Experiments with knock-out mice lacking prosaposin suggest that prosaposin helps maintain normal innervation patterns to the organ of Corti. PMID: 17167097
31. Combined saposin C and D deficiencies in mice lead to a neuronopathic phenotype. PMID: 17353235
32. These findings delineate the roles of saposin B for the in vivo degradation of several glycosphingolipid hydrolases and its primary function in maintenance of CNS function. PMID: 18480170
33. inactivation of the sortilin gene produces a significant decrease of prosaposin in the lysosomes PMID: 19732768

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KEGG: mmu:19156

STRING: 10090.ENSMUSP00000137476

UniGene: Mm.277498