Recombinant Mouse T-box transcription factor TBX21(Tbx21)

Code CSB-YP862395MO
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Source Yeast
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Code CSB-EP862395MO
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Source E.coli
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Code CSB-EP862395MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862395MO
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Source Baculovirus
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Code CSB-MP862395MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Tbx21
Uniprot No. Q9JKD8
Alternative Names Tbx21; Tbet; Tblym; T-box transcription factor TBX21; T-box protein 21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM
Species Mus musculus (Mouse)
Expression Region 1-530
Target Protein Sequence MGIVEPGCGD MLTGTEPMPS DEGRGPGADQ QHRFFYPEPG AQDPTDRRAG SSLGTPYSGG ALVPAAPGRF LGSFAYPPRA QVAGFPGPGE FFPPPAGAEG YPPVDGYPAP DPRAGLYPGP REDYALPAGL EVSGKLRVAL SNHLLWSKFN QHQTEMIITK QGRRMFPFLS FTVAGLEPTS HYRMFVDVVL VDQHHWRYQS GKWVQCGKAE GSMPGNRLYV HPDSPNTGAH WMRQEVSFGK LKLTNNKGAS NNVTQMIVLQ SLHKYQPRLH IVEVNDGEPE AACSASNTHV FTFQETQFIA VTAYQNAEIT QLKIDNNPFA KGFRENFESM YASVDTSVPS PPGPNCQLLG GDPFSPLLSN QYPVPSRFYP DLPGQPKDMI SQPYWLGTPR EHSYEAEFRA VSMKPTLLPS APGPTVPYYR GQDVLAPGAG WPVAPQYPPK MSPAGWFRPM RTLPMDPGLG SSEEQGSSPS LWPEVTSLQP EPSDSGLGEG DTKRRRISPY PSSGDSSSPA GAPSPFDKET EGQFYNYFPN
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs. Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation. Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells. Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. Inhibits the Th2 cell lineage commitment by suppressing the production of Th2 cytokines, such as IL-4, IL-5, and IL- 13, via repression of transcriptional regulators GATA3 and NFATC2. Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN- stimulated genes. Acts as a regulator of antiviral B-cell responses; controls chronic viral infection by promoting the antiviral antibody IgG2a isotype switching and via regulation of a broad antiviral gene expression program.
Gene References into Functions
  1. Data show that interleukin-2 inducible T cell kinase (Itk)negatively regulates the development of nTh1 cells that express interferon-gamma (IFNgamma) in a T-bet transcription factor (Tbet) independent manner. PMID: 28406139
  2. The enhanced Th1 and Th2 immune response in K + O immunized mice was also supported by the increased expression of Tbx21 and GATA-3 transcription factors in splenocytes. This corroborated with increased BLIMP-1 and Oct-2 protein expression. PMID: 29486619
  3. T-bet expression by T cells is not required for the induction of colitis or the differentiation of pathogenic Th17 cells but modifies qualitative features of the IL-23-driven colitogenic response by negatively regulating IL-23R expression. PMID: 27193261
  4. Phosphorylation of T-bet by RSK2 is required for IFNgamma expression for attenuation of colon cancer metastasis and growth. PMID: 29133416
  5. The Tbet pathway therefore directly impairs T-regulatory-cell reconstitution and is consequently a feasible target in efforts to prevent autoimmune graft-versus-host disease PMID: 28473623
  6. IL-12 promoted increasing T-bet and down regulating Eomes to promote the CD8+ T cell differentiation to memory T-cells in invasive pulmonary aspergillosis mouse model. PMID: 28924369
  7. The data are consistent with a looping/synapsing model for transcriptional regulation by T-bet in which a single dimer of the transcription factor can recognize and coalesce distinct genetic elements, either a promoter plus a distant regulatory element, or promoters on two different genes. PMID: 27791029
  8. Arid5a deficiency resulted in decreased levels of IFN-gamma under Th1 cell conditions, in which T-box expressed in T cells (T-bet) mRNA expression was inhibited. PMID: 27671645
  9. the model of mutually antagonistic differentiation programs driven by mutually exclusively expressed T-bet or GATA-3 does not completely explain natural CD4 T cell priming outcomes PMID: 29088218
  10. T-bet expression by Th cells is not required for the manifestation of T-cell-induced colitis in the presence of segmented filamentous bacteria and Helicobacter hepaticus. T-bet expression by Th cells controls their survival and localization, their repertoire of chemokine and chemokine receptor expression, the accumulation of monocytes and macrophages in the inflamed colon. PMID: 26883725
  11. T-bet acts through enhancers to allow the recruitment of Mediator and P-TEFb in the form of the super elongation complex. PMID: 27292648
  12. results suggest that T-bet(+) Treg cells have an essential immunosuppressive function and indicate that Treg cell functional heterogeneity is a critical feature of immunological tolerance PMID: 28607488
  13. results establish a novel mechanism by which mTORC1 regulates Th1 differentiation, through control of T-bet phosphorylation. PMID: 28424242
  14. Conditional deletion of T-bet from B cells impaired the formation of germinal centers and mitigated the development of kidney damage and rapid mortality in systemic lupus erythematosus mice. PMID: 28240602
  15. this study shows that T-bet restrains IFN-gamma-induced collateral type I IFN circuitry in the Th1 response in vivo PMID: 28623086
  16. Cell migration is impaired in T-bet deficient Th17 cells in the central nervous system. PMID: 27242075
  17. Our data suggest that T-bet might contribute to the remission of CIA by facilitating the regulatory potential of IL-10-positive MZ B cells. PMID: 27343199
  18. There is a genetic association of ankylosing spondylitis with TBX21 influences T-bet and pro-inflammatory cytokine expression in humans and SKG mice as a model of spondyloarthritis. PMID: 27125523
  19. Our findings suggest that T-bet over-expression-induced suppression of Th17 differentiation is mediated through IFN-gamma-independent AHR suppression. PMID: 27936495
  20. this study reveales a new mechanism through which procyanidin B2 gallates inhibit IFN-gamma and IL-17 production in T cells by down-regulation of T-bet and RORgammat expression PMID: 28088699
  21. the key role of the transcription factor c-Myb in regulating the T-bet-mediated anti-viral program, is reported. PMID: 28423310
  22. T-bet regulates natural regulatory T cells migration into afferent lymphatics and lymph nodes and consequently their suppressive stability in vivo PMID: 26880765
  23. These data advance our understanding of the transcriptional regulation of megakaryopoiesis by supporting a new role for T-bet in the differentiation of MEPs into megakaryocytes. PMID: 26607595
  24. T-bet Promotes Acute Graft-versus-Host Disease by Regulating Recipient Hematopoietic Cells in Mice PMID: 26903480
  25. the effect of T-bet deletion in apolipoproteinE (ApoE) knockout mice fed a high fat diet (HFD) or normal chow diet, was studied. PMID: 26886778
  26. Dominance of regulatory T cells in carcinogen-induced fibrosarcomas is not T-bet or Il-2 dependent. PMID: 26433463
  27. a critical role for IRF4 in regulating protective anti-viral CD8+ T cell responses by ensuring a balanced ratio of T-bet to Eomes PMID: 26714260
  28. Cytokines analysis in peritoneal fluid showed that IFN-gamma was significantly increased in chlorhexidine gluconate (CG)-injected T-bet Tg mice and that IL-13 was significantly increased in CG-injected GATA-3 Tg mice PMID: 26156402
  29. Our data indicate that Tim-3 expression on NK cells is regulated by T-bet, and that Tim-3 levels correlate with advanced stages of gastric cancer PMID: 26214042
  30. is associated with the increased expression of T-bet in T cells PMID: 26461455
  31. Data show that although T and B cell infiltration into the central nervous system of infected T-bet transcription factor knockout (T-bet-/-) mice is comparable, their activities are not, resulting in delayed, low-level antibody production . PMID: 26408670
  32. T-bet is required for Th1 differentiation and migration, as well as for optimal function of Th17 cells. PMID: 25404360
  33. Studies indicate that a key feature of age-associated B cells (ABCs) is that they express and depend upon B cell-intrinsic expression of the T-bet transcription factor. PMID: 26297793
  34. T-bet as a key transcriptional regulator of tumor-reactive CD8(+) T-cell effector differentiation under otherwise tolerizing conditions. PMID: 25516478
  35. methyl-CpG-binding domain protein 2 (MBD2), an epigenetic regulator, controls autoimmunity and EAE through T-bet/Hlx. PMID: 24934598
  36. T-bet-deficient recipients of complete MHC-mismatched lung allografts develop costimulation blockade-resistant rejection characterized by neutrophilia and obliterative airway inflammation that is predominantly mediated by CD8(+)IL-17(+) T cells. PMID: 25286244
  37. Data show that transcription factor T-bet expression level was lower in OCH glycolipid treatment group than in bone marrow failure (BMF) group. PMID: 25254224
  38. these data highlight T-bet-independent pathways to IFN-gamma production and reveal a novel role for this transcription factor in coordinating the T cell responses necessary to control this infection in peripheral tissues. PMID: 25556247
  39. These findings reveal an unexpected link between T-bet overexpression in T lymphocytes and the development of pulmonary alveolar proteinosis caused by reorganization of mononuclear phagocytes in the lung PMID: 25349175
  40. Inflammation in acute microbial infection induced T-bet and Eomes. It promoted effector differentiation of self-reactive T cells in tolerance-favoring conditions. In Listeriosis, these responses relied on elevated T-bet expression, independent of Eomes. PMID: 24823810
  41. T-bet stimulates epigenetic remodeling of Tmevpg1-specific enhancers and Ifng-specific enhancers to achieve Th1-lineage-specific expression of Ifng. PMID: 25225667
  42. T-bet undergoes several post-translational protein modifications, such as phosphorylation and ubiquitination, which affect lineage commitment during T-cell differentiation. (Review) PMID: 24901011
  43. This study reveals a function of T-bet as a central transcriptional regulator linking agonist selection and IL-15 signaling with the emergence of CD8alphaalpha(+) intraepithelial lymphocytes. PMID: 25148024
  44. cooperation between the transcription factors T-bet and Runx3 resulted in suppression of conventional CD4(+) T helper functions and induction of an intraepithelial lymphocyte (IEL) program that included expression of IEL markers such as CD8alphaalpha homodimers. PMID: 25148025
  45. T-bet modulates the antibody response and immune protection during murine malaria. PMID: 25047384
  46. The role of T-bet in promoting the course of the disease demonstrated that T-bet was a critical factor in collagen-induced arthritis development. PMID: 24425064
  47. These findings identify T-bet as a potentially important susceptibility factor for nickel nanoparticle exposure PMID: 24499286
  48. Nod/Ripk2 signaling in dendritic cells activates IL-17A-secreting innate lymphoid cells and drives colitis in T-bet-/-.Rag2-/- (TRUC) mice. PMID: 24927559
  49. Ezh2 was also required to prevent proteasome-mediated degradation of T-bet protein in Th1 cells. PMID: 24760151
  50. The transcription factors T-bet and Runx are required for the ontogeny of pathogenic interferon-gamma-producing T helper 17 cells. PMID: 24530058

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Subcellular Location Nucleus.
Tissue Specificity T-cell specific. Expressed in regulatory T (TReg) cells.
Database Links

KEGG: mmu:57765

STRING: 10090.ENSMUSP00000001484

UniGene: Mm.94519

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