Recombinant Mouse TFIIH basal transcription factor complex helicase XPD subunit(Ercc2)

Code CSB-YP007770MO
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Source Yeast
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Code CSB-EP007770MO
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Source E.coli
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Code CSB-EP007770MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007770MO
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Source Baculovirus
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Code CSB-MP007770MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Ercc2
Uniprot No. O08811
Alternative Names Ercc2; XpdGeneral transcription and DNA repair factor IIH helicase subunit XPD; TFIIH subunit XPD; EC 3.6.4.12; CXPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; Xeroderma pigmentosum group D-complementing protein
Species Mus musculus (Mouse)
Expression Region 1-760
Target Protein Sequence MKLNVDGLLV YFPYDYIYPE QFSYMLELKR TLDAKGHGVL EMPSGTGKTV SLLALIVAYQ RAYPLEVTKL IYCSRTVPEI EKVIEELRKL LSFYEQQEGE KLPFLGLALS SRKNLCIHPE VTPLRFGKDV DGKCHSLTAS YVRAQYQQDA SLPHCRFYEE FDIHGRQMPL PAGIYNLDDL KALGQRQGWC PYFLARYSIL HANVVVYSYH YLLDPKIADL VSKELARKAV VVFDEAHNID NVCIDSMSVN LTRRTLDRCQ SNLDTLQKTV LRIKETDEQR LRDEYRRLVE GLREASVARE TDAHLANPVL PDEVLQEAVP GSIRTAEHFL GFLRRLLEYV KWRLRVQHVV QESPPAFLSG LAQRVCIQRK PLRFCAERLR SLLHTLEIAD LADFSPLTLL ANFATLVSTY AKGFTIIIEP FDDRTPTIAN PVLHFSCMDA SLAIKPVFER FQSVIITSGT LSPLDIYPKI LDFHPVTMAT FTMTLARVCL CPMIIGRGND QVAISSKFET REDIAVIRNY GNLLLEMSAV VPDGIVAFFT SYQYMESTVA SWYEQGILEN IQRNKLLFIE TQDGAETSVA LEKYQEACEN GRGAILLSVA RGKVSEGIDF VHHYGRAVIM FGVPYVYTQS RILKARLEYL RDQFQIREND FLTFDAMRHA AQCVGRAIRG KTDYGLMVFA DKRFARADKR GKLPRWIQEH LTDSNLNLTV DEGVQVAKYF LRQMAQPFHR EDQLGLSLLS LEQLQSEETL QRIEQIAQQL
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.
Gene References into Functions
  1. Results describe a recessive cataract caused by a mutation in the Xpd/Ercc2 gene and demonstrate the importance of the gene not only for lens fiber cell differentiation, but also for the sensitivity to ionizing radiation. PMID: 25951169
  2. Compound TTD/XPCS heterozygosity partially rescues metabolic phenotype associated with homozygous XPD alleles. TTD allele dominates over XPCS allele in measures of UV-sensitivity. PMID: 23046824
  3. premature aging associated with XPD mutation in mice PMID: 11950998
  4. Mutations in XPD subunit of TFIIH result in transactivation defect of PPARs in the adipose tissue and the liver. PMID: 15988019
  5. New pathology features support the premature aging phenotype of Xpd(TTD) mutant mice and further strengthen the link between DNA damage, DNA repair and aging. PMID: 16115803
  6. XPCS with a G602D-encoding mutation in the Xpd helicase gene is the most skin cancer-prone NER model to date, and it shows an unusual NER dysfunction that is likely responsible for this susceptibility PMID: 16904611
  7. a variety of biallelic effects on organismal phenotype which attributes to combinations of recessive Xpd alleles PMID: 17020410
  8. results support a general model for premature aging in Xpd DNA repair deficient mice based on cellular responses to DNA damage that impair normal tissue homeostasis PMID: 18545656
  9. ERCC2 plays role in lung cancer development in a Chinese population. PMID: 19406934

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Subcellular Location Nucleus. Cytoplasm, cytoskeleton, spindle.
Protein Families Helicase family, RAD3/XPD subfamily
Database Links

KEGG: mmu:13871

STRING: 10090.ENSMUSP00000054380

UniGene: Mm.36524

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