Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Trex1

Uniprot No.

Q91XB0

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

Trex1; Three-prime repair exonuclease 1; EC 3.1.11.2; 3'-5' exonuclease TREX1

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-314aa

Target Protein Sequence

MGSQTLPHGHMQTLIFLDLEATGLPSSRPEVTELCLLAVHRRALENTSISQGHPPPVPRPPRVVDKLSLCIAPGKACSPGASEITGLSKAELEVQGRQRFDDNLAILLRAFLQRQPQPCCLVAHNGDRYDFPLLQTELARLSTPSPLDGTFCVDSIAALKALEQASSPSGNGSRKSYSLGSIYTRLYWQAPTDSHTAEGDVLTLLSICQWKPQALLQWVDEHARPFSTVKPMYGTPATTGTTNLRPHAATATTPLATANGSPSNGRSRRPKSPPPEKVPEAPSQEGLLAPLSLLTLLTLAIATLYGLFLASPGQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

41.1 kDa

Protein Length

Full length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer，50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Unless degraded, these DNA fragments accumulate in the cytosol and activate the IFN-stimulatory DNA (ISD) response and innate immune signaling. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

Gene References into Functions

Trex1 is an upstream regulator of radiation-driven anti-tumor immunity. Trex1 induction may guide the selection of radiation dose and fractionation in patients treated with immunotherapy. PMID: 28598415
the cell-cycle-dependent post-translation modification of TREX1 regulates its interaction with OST. PMID: 28297665
The effect of topical TREX1 knockdown and local interferon production on HIV transmission in human cervicovaginal explants and humanized mice, is reported. PMID: 27184854
data do not support the concept of retroelement-derived cDNA as key triggers of systemic autoimmunity in Trex1-deficient humans and mice PMID: 28835460
Trex1 expression in dendritic cells is essential to prevent breakdown of self-tolerance ensuing from aberrant detection of endogenous DNA. PMID: 27511730
Data show that oligosaccharyltransferase (OST) activity is dysregulated in three prime exonuclease 1 knockout (Trex1-/-) cells. PMID: 26320659
Data show that cyclic GMP-AMP synthase (cGAS) is essential for all aspects of the autoimmune disease in 3' repair exonuclease Trex1 knockout mice. PMID: 26223655
Dysfunctional dsDNA degradation by TREX1 D18N induces disease in mice that recapitulates many characteristics of human lupus. PMID: 25848017
knocking out the DNA sensor cyclic GMP-AMP synthase completely abrogates spontaneous induction of IFN-stimulated genes in TREX1-deficient cells. PMID: 24813208
Spontaneous type I INF dependent cutaneous pathology in TREX1 deficiency illustrates common pathogenetic pathway in chilblain lupus. PMID: 24270665
Upon proinflammatory stimulation, Trex1(-/-) macrophages increase CD86, TNF-alpha & IFN-alpha production, & Ag presentation to CD4(+) T cells, but decrease apoptotic T cell clearance. Trex1 is a negative regulator of macrophage inflammatory activation. PMID: 24218451
Oxidized DNA Is less susceptible to TREX1 degradation; the oxidized base 8-hydroxyguanosine, a marker of oxidative damage in DNA, potentiated cytosolic immune recognition by decreasing its susceptibility to 3' repair exonuclease 1 -mediated degradation PMID: 23993650
regulates lysosomal biogenesis and interferon-independent activation of antiviral genes PMID: 23160154
The structures of the mutant TREX1 proteins provide insight into the dysfunction relating to human disease. PMID: 22071149
TREX1 bound to cytosolic HIV DNA and digested excess HIV DNA that would otherwise activate interferon expression via a pathway dependent on the kinase TBK1, the adaptor STING and the transcription factor IRF3 PMID: 20871604
Trex1(-/-) mice exhibit a dramatically reduced survival and develop inflammatory myocarditis leading to progressive, often dilated, cardiomyopathy and circulatory failure PMID: 15254239
Study defines Trex1 as an essential negative regulator of the IFN-stimulatory DNA response and delineate the genetic pathway linking Trex1 deficiency to lethal autoimmunity. PMID: 18724932
Study showed that GFP-TREX1-(1-307) lacking the C-terminal seven amino acids localizes in a perinuclear pattern. PMID: 19442247

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Subcellular Location

Nucleus. Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein. Note=Retained in the cytoplasm through the C-terminal region. In response to DNA damage, translocates to the nucleus where it is specifically recruited to replication foci. Translocation to the nucleus also occurs during GZMA-mediated cell death.

Protein Families

Exonuclease superfamily, TREX family

Tissue Specificity

Widely expressed with high expression levels detected in spleen, thymus and uterus.

Database Links

KEGG: mmu:22040

STRING: 10090.ENSMUSP00000050971

UniGene: Mm.439964

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Code	CSB-EP821009MO
MSDS	MSDS
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Three-prime repair exonuclease 1 (Trex1)

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