Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Mafk

Uniprot No.

Q61827

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

Mafk; Nfe2u; Transcription factor MafK; Erythroid transcription factor NF-E2 p18 subunit

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-156aa

Target Protein Sequence

MTTNPKPNKALKVKKEAGENAPVLSDDELVSMSVRELNQHLRGLTKEEVTRLKQRRRTLKNRGYAASCRIKRVTQKEELERQRVELQQEVEKLARENSSMRLELDALRSKYEALQTFARTVARGPVTPTKVATTSVITIVKSAELSSTSVPFSAAS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

37.5 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Constructing a plasmid encoding the Mouse Mafk protein (1-156aa) initiates the general approach for generating the recombinant Mouse Mafk protein. Transformation of the plasmid into e.coli cells obtains the plasmid-containing e.coli cells, which are cultured and induced for protein expression. A N-terminal 10xHis-SUMO tag and C-terminal Myc tag is fused to the protein. Subsequently, the protein is purified through affinity purification, and SDS-PAGE analysis is undertaken to confirm the presence and assess the purity of the protein. The protein's purity exceeds 85%.

Target Background

Function

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.

Gene References into Functions

beta-Cell-Specific Mafk Overexpression Impairs Pancreatic Endocrine Cell Development PMID: 26901059
KAP-1 may contribute to the repression of Ey and beta-major globin gene transcription through recruitment to the promoters of these two genes, mediated by the interaction of KAP-1 with either Zfp445 or MafK, respectively PMID: 23291531
regulation of dynamic exchange with Bach1 PMID: 14747657
mafG::mafK::mafF triple-mutant fibroblasts that completely lack small Mafs are highly susceptible to oxidative stress. (mafK) PMID: 16135796
DNA-binding activity of endogenous MafA was significantly increased in the MafK transgenic mice PMID: 16780794
The involvement of both HNF4alpha and NF-E2 in Abcc6 gene regulation suggests that Abcc6 might be involved in a detoxification processes related to hemoglobin or heme. PMID: 16997394
MafK/NF-E2 p18 recruitment was involved in the physical proximity of LCR and active beta-globin genes upon beta-globin gene transcriptional activation. PMID: 18308612
Histological analysis revealed that embryonic development of beta cells in the MafA(-/-)MafK(+) mice was significantly suppressed and the reduced number of beta cells was responsible for the early onset of diabetes. PMID: 19715672

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Subcellular Location

Nucleus.

Protein Families

BZIP family, Maf subfamily

Tissue Specificity

Highly expressed in heart, skeletal muscle and placenta. Also expressed in erythroid cells.

Database Links

KEGG: mmu:17135

STRING: 10090.ENSMUSP00000018287

UniGene: Mm.157313

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Code	CSB-EP713990MO
Abbreviation	Recombinant Mouse Mafk protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Transcription factor MafK (Mafk)

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