Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Activity

Not Test

Target Names

Tpbg

Uniprot No.

Q9Z0L0

Research Area

Cancer

Alternative Names

5T4 oncofetal trophoblast glycoprotein;Wnt-activated inhibitory factor 1

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

32-361aa

Target Protein Sequence

SAPSSSVPSSSTSPAAFLASGSAQPPPAERCPAACECSEAARTVKCVNRNLLEVPADLPPYVRNLFLTGNQMTVLPAGAFARQPPLADLEALNLSGNHLKEVCAGAFEHLPGLRRLDLSHNPLTNLSAFAFAGSNASVSAPSPLEELILNHIVPPEDQRQNGSFEGMVAFEGMVAAALRSGLALRGLTRLELASNHFLFLPRDLLAQLPSLRYLDLRNNSLVSLTYASFRNLTHLESLHLEDNALKVLHNSTLAEWHGLAHVKVFLDNNPWVCDCYMADMVAWLKETEVVPDKARLTCAFPEKMRNRGLLDLNSSDLDCDAVLPQSLQTS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

37.1 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

Gene References into Functions

The surface expression of 5T4 marks the use of the CXCR4 rather than the CXCR7 receptor, with distinct consequences for CXCL12 exposure. PMID: 22956548
5T4 overexpression in newborn olfactory bulb interneurons facilitates their dendritic arborization even under a sensory input-deprived condition. PMID: 22323733
molecular interaction of 5T4 and CXCR4 occurring at the cell surface which directly facilitates the biological response to CXCL12 PMID: 20376365
Spry4 and Tpbg/5T4 were detected in Kit-immunoreactive cells only in Kit(K641E), but not in Kit(WT/WT) animals. PMID: 19453770
Glycosylation and epitope mapping of the 5T4 glycoprotein oncofoetal antigen PMID: 11903056
5T4 glycoprotein expression is associated with early differentiative events of embryonic stem cells PMID: 14576347
A limited expression profile of 5T4 may contribute to the migration of actively cycling, undifferentiated epithelial progenitor cells during embryogenesis PMID: 15977177
Because 5T4 is known to interact with actin cytoskeleton, these observations suggest that 5T4 is involved in the formation or maintenance of strata-specific dendritic ramification or synaptic connection of subsets of local interneurons. PMID: 16506198
E-cadherin protein stabilizes cortical actin cytoskeletal arrangement in embryonic stem cells, and this can prevent cell surface localization of the promigratory 5T4 antigen. PMID: 17507657

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Tissue Specificity

Highly expressed in embryo and placenta. In adult, expressed only in brain and ovary. Not detected in kidney small intestine, heart, spleen, testis, liver, lung, thymus and stomach.

Database Links

KEGG: mmu:21983

STRING: 10090.ENSMUSP00000006559

UniGene: Mm.20864

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Code	CSB-MP7221MO
Abbreviation	Recombinant Mouse Tpbg protein, partial
MSDS	MSDS Datasheet
Size	$138
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Trophoblast glycoprotein (Tpbg), partial

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