Recombinant Mouse Tumor necrosis factor receptor type 1-associated DEATH domain protein (Tradd)

Code CSB-YP659708MO
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Source Yeast
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Code CSB-EP659708MO
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Source E.coli
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Code CSB-EP659708MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP659708MO
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Source Baculovirus
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Code CSB-MP659708MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Tradd
Uniprot No.
Alternative Names
Tradd; Tumor necrosis factor receptor type 1-associated DEATH domain protein; TNFR1-associated DEATH domain protein; TNFRSF1A-associated via death domain
Species
Mus musculus (Mouse)
Expression Region
1-310
Target Protein Sequence
MAAGQNGHEE WVGSAYLFLE SAVDKVILSE AYTDPKKKVA IYKALQTALS ESGDSSDVLQ ILKIHCSDPQ LIVQLRFCGR VLCGRFLQAY REGALRTALQ RCMAPALAQE ALRLQLELRA GAEQLDSWLT DEERCLNYIL AQKPDRLRDE ELAELEDELC KLTCDCTGQG GAIQVASAGS KFPVSSPTEE KPLPAACQTF LFHGQLVVNR PLTLQDQQTF ARSVGLKWRR VGRSLQRNCR ALRDPALDSL AYEYERDGLY EQAFQLLRRF MQAEGRRATL QRLVEALEEN ELTSLAEDLL GQAEPDGGLA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A.
Gene References into Functions
  1. TRADD knockout blunts pressure overload-induced cardiac hypertrophy through mediating TAK1/p38 MAPK but not AKT phosphorylation PMID: 28013046
  2. Data indicate that ASK1 activation stimulated the activity of the transcription factor FoxO3a, which increased the abundance of the apoptosis-promoting adaptor protein TRADD, leading to activation of caspase 8. PMID: 23982205
  3. data indicate that TRADD shuttles dynamically from the cytoplasm into the nucleus to modulate the interaction between p19(Arf) and its E3 ubiquitin ligase ULF, thereby promoting p19(Arf) protein stability and tumour suppression PMID: 22561347
  4. Data suggest that deficiency of TRADD sensitizes cells to TRAIL-induced apoptosis, and that enhanced cell death in TRADD(-/-) MEFs is associated with defective NF-kappaB activation. PMID: 21627969
  5. TRADD is required for recruitment of receptor interacting protein 1 and TNFR-associated factor 2 to the DR3 signaling complex and for the ubiquitination of receptor interacting protein 1 PMID: 21421854
  6. We show that TRADD is recruited to the TRAIL-receptor complex, and RIP1 recruitment is mediated by TRADD. PMID: 21187341
  7. TRADD may be involved in IFN-gamma signaling by forming a complex with STAT1-alpha within the nucleus and regulating IFN-gamma-mediated STAT1-alpha activation. PMID: 14730360
  8. Tradd activates distinct mechanisms of apoptosis from the nucleus and the cytoplasm. PMID: 15761471
  9. silencing TRADD expression with small-interfering RNA reduced neuronal apoptosis and subsequent microglial and astroglial activation PMID: 18325634
  10. TRADD is a multifunctional protein crucial both for TNFR1 signaling and other signaling pathways relevant to immune responses. PMID: 18719121

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Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton.
Database Links
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