Product Details

Purity

>85% (SDS-PAGE)

Target Names

Atp6v0d2

Uniprot No.

Q80SY3

Alternative Names

Atp6v0d2; V-type proton ATPase subunit d 2; V-ATPase subunit d 2; Osteoclast-specific vacuolar ATP synthase; Vacuolar proton pump subunit d 2

Species

Mus musculus (Mouse)

Expression Region

1-350

Target Protein Sequence

MLETAELYFN VDHGYLEGLV RGCKASLLTQ QDYVNLVQCE TLEDLKIHLQ TTDYGNFLAN ETNPLTVSKI DTEMRKKLCR EFDYFRNHSL EPLSTFLTYM TCSYMIDNII LLMNGALQKK SVKEVLAKCH PLGRFTEMEA VNIAETPSDL FKAVLVETPL APFFQDCMSE NTLDELNIEL LRNKLYKSYL EAFYKFCKDH GDVTADVMCP ILEFEADRRA LIITLNSFGT ELSKEDRETL FPTCGRLYPE GLRLLAQAED FEQMKRVADN YGVYKPLFDA VGGSGGKTLE DVFYEREVQM NVLAFNRQFH YGVFYAYVKL KEQEMRNIVW IAECISQRHR TKINSYIPIL

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis. Regulator of osteoclast fusion and bone formation.

Gene References into Functions

High V-ATPase expression is associated with Bone Pain Induced by Multiple Myeloma. PMID: 28254863
we propose that PTH has a direct effect on osteoblasts and osteoclasts, and that this effect is mediated through PTH1R, thus contributing to bone remodeling PMID: 26647715
Insulin significantly activated ERK1/2 MAP kinase as well as markedly induced the expression of NFATc1, an osteoclast marker gene, and Atp6v0d2, an osteoclast fusion-related gene. PMID: 26343857
V-ATPase has a role in modulating a macrophage phenotype towards tumor-associated macrophages through the action of the a2 isoform of V-ATPase PMID: 24362525
Luteolin can be effective in reducing bone resorption and that this effect of luteolin may be through disruption of osteoclast V-ATPase a3-d2 interaction. PMID: 23129004
PKCdelta deficiency does not perturb formation of the ruffled border or trafficking of lysosomal vesicles containing the vacuolar-ATPase (v-ATPase). PMID: 22806935
these findings suggest that the critical role of ATP6v0d2 may be limited to the control of bone homeostasis under normal development. PMID: 21040703
The data indicate that integrity of lipid rafts regulates the activity of V-ATPase in osteoclasts, suggesting that cholesterol-lowering agents might be useful for inhibiting osteoclast-dependent bone resorption. PMID: 20127130
d2 is expressed mainly in kidney and at lower levels in heart, spleen, skeletal muscle, and testis. The d2 isoform can complement the yeast vma6Delta phenotype when cells are grown at 25, but not 30, degrees C. PMID: 12963731
d2 mRNA and protein appear later during nephrogenesis than does the ubiquitously expressed E1 subunit PMID: 15800125
d2 protein displayed association with membranes and the localization in lysosomes and antigen-containing endosomes. PMID: 16144709
Atp6v0d2 is a regulator of osteoclast fusion and bone formation. PMID: 17128270
Data indicate for the first time that the NFATc1/Atp6v0d2 and DC-STAMP signaling axis plays a key role in the osteoclast multinucleation process, which is essential for efficient bone resorption. PMID: 17885208
Atp6v0d2 (d2), an isoform of the d subunit in the V-ATPase, showed 5-fold higher expression than that of Atp6v0d1 (d1) in mature osteoclasts, indicating a potential function in osteoclastic bone resorption. PMID: 19113919
MEF2 and MITF function cooperatively with NFATc1 to transactivate the V-ATPase d2 promoter during RANKL-induced osteoclastogenesis. PMID: 19321441

Hide All

Protein Families

V-ATPase V0D/AC39 subunit family

Tissue Specificity

Predominantly expressed in the kidney. Also expressed in the lung, testis, skeletal muscle and heart. Upr-egulated during osteoclast differentiation and is most abundant in mature osteoclasts.

Database Links

KEGG: mmu:242341

STRING: 10090.ENSMUSP00000029900

UniGene: Mm.19298

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-YP770449MO
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP770449MO
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP770449MO-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP770449MO
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP770449MO
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Mouse V-type proton ATPase subunit d 2 (Atp6v0d2)

Product Details

Customer Reviews and Q&A

Target Background

×CloseMessage