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Recombinant Mouse Ventricular zone-expressed PH domain-containing protein 1 (Veph1), partial

Recombinant Mouse Ventricular zone-expressed PH domain-containing protein 1 (Veph1), partial

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Code	CSB-EP025844MO
Abbreviation	Recombinant Mouse Veph1 protein, partial
MSDS	MSDS Datasheet
Size	US$388
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Image	Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP025844MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Veph1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP025844MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Veph1. (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Veph1

Uniprot No.

A1A535

Research Area

Others

Alternative Names

Veph1; Veph; Ventricular zone-expressed PH domain-containing protein 1; Protein melted homolog

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

660-833aa

Target Protein Sequence

AMTESTFPQQKDLEQLQLHLEEVRFFDVFGFSETAGAWQCFMCNNPEKATVVNQDGQPLIEGKLKEKQVRWKFIKRWKTHYFTLAGNQLLFQKGKSKDDPDDSPIELSKVQSVKAVAKKRRDRSLPRAFEIFTDSKTYVFKAKDEKNAEEWLQCINVALAQAKERESREVTTYL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

27.7 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombination of a plasmid encoding the Mouse Veph1 protein (660-833aa) is the first step during the production the recombinant Mouse Veph1 protein. The constructed plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected to be cultured for the induction of protein expression. The protein is equipped with a N-terminal 10xHis tag and C-terminal Myc tag. After expression, affinity purification is used to isolate and purify the recombinant Mouse Veph1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Mouse Veph1 protein. Its purity exceeds 85%.

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Target Background

Function

Interacts with TGF-beta receptor type-1 (TGFBR1) and inhibits dissociation of activated SMAD2 from TGFBR1, impeding its nuclear accumulation and resulting in impaired TGF-beta signaling. May also affect FOXO, Hippo and Wnt signaling.

Gene References into Functions

Veph was strongly expressed in the ventricular zone of the embryonic brain, and its expression level gradually decreased in later stages. PMID: 15388229

Subcellular Location

Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

MELT/VEPH family

Tissue Specificity

Specifically expressed in kidney and eye. In the eye, expressed in retinal pigmented epithelium but not in the neural retina.

Database Links

KEGG: mmu:72789

STRING: 10090.ENSMUSP00000029419

UniGene: Mm.443526

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