Recombinant Mouse Ventricular zone-expressed PH domain-containing protein 1 (Veph1), partial

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Code CSB-EP025844MO
Size US$388
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  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP025844MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Veph1.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP025844MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Veph1.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Veph1
Uniprot No.
Research Area
Others
Alternative Names
Veph1; Veph; Ventricular zone-expressed PH domain-containing protein 1; Protein melted homolog
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
660-833aa
Target Protein Sequence
AMTESTFPQQKDLEQLQLHLEEVRFFDVFGFSETAGAWQCFMCNNPEKATVVNQDGQPLIEGKLKEKQVRWKFIKRWKTHYFTLAGNQLLFQKGKSKDDPDDSPIELSKVQSVKAVAKKRRDRSLPRAFEIFTDSKTYVFKAKDEKNAEEWLQCINVALAQAKERESREVTTYL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
27.7 kDa
Protein Length
Partial
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Recombination of a plasmid encoding the Mouse Veph1 protein (660-833aa) is the first step during the production the recombinant Mouse Veph1 protein. The constructed plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected to be cultured for the induction of protein expression. The protein is equipped with a N-terminal 10xHis tag and C-terminal Myc tag. After expression, affinity purification is used to isolate and purify the recombinant Mouse Veph1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Mouse Veph1 protein. Its purity exceeds 85%.

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Target Background

Function
Interacts with TGF-beta receptor type-1 (TGFBR1) and inhibits dissociation of activated SMAD2 from TGFBR1, impeding its nuclear accumulation and resulting in impaired TGF-beta signaling. May also affect FOXO, Hippo and Wnt signaling.
Gene References into Functions
  1. Veph was strongly expressed in the ventricular zone of the embryonic brain, and its expression level gradually decreased in later stages. PMID: 15388229
Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
MELT/VEPH family
Tissue Specificity
Specifically expressed in kidney and eye. In the eye, expressed in retinal pigmented epithelium but not in the neural retina.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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