Recombinant Mouse cGMP-inhibited 3',5'-cyclic phosphodiesterase B (Pde3b), partial

Code CSB-YP720247MO
MSDS
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Source Yeast
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Code CSB-EP720247MO
MSDS
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Source E.coli
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Code CSB-EP720247MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP720247MO
MSDS
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Source Baculovirus
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Code CSB-MP720247MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pde3b
Uniprot No.
Alternative Names
Pde3b; cGMP-inhibited 3',5'-cyclic phosphodiesterase B; EC 3.1.4.17; CGIPDE1; Cyclic GMP-inhibited phosphodiesterase B; CGI-PDE B
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.
Gene References into Functions
  1. PDE3B and/or its downstream signaling partners might be important regulators of energy metabolism in adipose tissue. PMID: 28084425
  2. NLRP3 inflammasome-mediated increase of IL1beta , TNFalpha and IL12-p40 in response to Lipopolysaccharides-associated danger signals participate in the development of proinflammatory state, leading to insulin resistance. PMID: 27321128
  3. Study concludes that CUGBP1 is a critical regulator of insulin secretion via activating PDE3B. PMID: 27255754
  4. Insulin induction of hypothalamic phosphodiesterase 3B activity and the reversal of the anorectic and body weight-reducing effects and stimulatory effect of insulin on hypothalamic proopiomelanocortin gene expression by cilostamide suggest that activation of phosphodiesterase 3B is a novel mechanism of insulin signaling in the hypothalamus. PMID: 27002827
  5. Suggest that by reducing production of cAMP in adipocytes, PDE3B, IKKepsilon and TBK1 may contribute to the repression of energy expenditure during obesity. PMID: 24368730
  6. Lack of PDE3B in brown adipocytes impairs the action of insulin on lipolysis. PMID: 26031333
  7. these findings indicate that PDE3B deletion confers cardioprotective effects because of cAMP/PKA-induced preconditioning, which is associated with the accumulation of proteins with cardioprotective function in ICEFs. PMID: 25877153
  8. Data suggest that Pde3b is involved in regulation of adipogenesis of adult stem cells into white adipocytes versus brown adipocytes; knockout mice lacking Pde3b form epididymal white adipose tissue with characteristics of brown adipose tissue. PMID: 23766131
  9. The variation of PDE3B-expression in the brain might play a crucial pathophysiological role, and regulation of PDE3B production might protect against ischemic brain damage. PMID: 23471014
  10. Calcineurin regulates degradation of phosphodiesterase 3B, in addition to phosphodiesterase 4D. PMID: 23258544
  11. The PDE3B pathway has a direct role in mediating leptin signaling in proopiomelanocortin and neuropeptide Y neurons, via a potential mechanism of leptin signaling in the hypothalamus. PMID: 22001576
  12. Results suggest that cav-1 acts as a molecular chaperone or scaffolding molecule in cholesterol-rich lipid rafts that may be necessary for the proper stabilization and activation of PDE3B in response to CL and insulin. PMID: 19747167
  13. Differential regulation of gene expression and insulin-induced activation of this enzyme in adipocytes of lean insulin-resistant IRS-1 (-/-) mice. PMID: 12213348
  14. Regulation of phosphodiesterase 3B gene expression by peroxisome proliferator-activated receptor gamma. PMID: 12729899
  15. PDE3B has an essential role in cAMP-mediated regulation of insulin release and glucose homeostasis PMID: 14736883
  16. p87PIKAP is a novel regulatory subunit of phosphoinositide 3-kinase gamma that is highly expressed in heart and interacts with PDE3B [p87PIKAP] PMID: 16476736
  17. PDE3B, a key enzyme in insulin signalling, appears to be associated with caveolae in adipocytes and this localization seems to be functionally important. PMID: 16503395
  18. These results indicate that AS47 exists in a complex with PDE3B in adipocytes and that the amount of AS47 protein regulates the amount of PDE3B. PMID: 16516160
  19. cAMP-response element-binding protein has a role in regulation of expression of the murine Pde3b gene in differentiating 3T3-L1 preadipocytes PMID: 16702214
  20. Phosphorylation of PDE3B after treatment of cells with insulin, cAMP-increasing agents, or the phosphatase inhibitor, calyculin A was analyzed by two-dimensional tryptic phosphopeptide mapping and mass spectrometry. PMID: 17320989
  21. PDE3B, through hydrolysis of cAMP in pools regulated by Ca(2+), plays a regulatory role in depolarization-induced insulin secretion and that the enzyme is associated with the exocytotic machinery in beta-cells. PMID: 17368848
  22. Role of Pde3b in regulating platelet and cardiac function using subtype-selective knockout mice is reported. PMID: 17482796
  23. PKB is required for the positive effects of insulin on lipid storage and regulation of PDE3B and AMPK by PKB is important for these effects. PMID: 19158325
  24. hepatocyte PDE3B is localized in caveolae and smooth endoplasmic reticulum and plays important roles in the regulation of glucose, triglyceride and cholesterol metabolism PMID: 19262749

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Cyclic nucleotide phosphodiesterase family, PDE3 subfamily
Tissue Specificity
Abundant in adipose tissues.
Database Links
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