Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
(ATase(Glutamine phosphoribosylpyrophosphate amidotransferase)(GPATase)
Species
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Expression Region
35-527aa
Target Protein Sequence
CGVFGVWAPGEDVAKLTYYGLYALQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLAAMQGHVAIGHCRYSTTGDTTWENAQPVFRNTAAGTGVALGHNGNLVNAAALAARARDAGLIATRCPAPATTDSDILGALLAHGAADSTLEQAALDLLPTVRGAFCLTFMDENTLYACRDPYGVRPLSLGRLDRGWVVASETAALDIVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCVFEYVYLARPDSTIAGRSVHAARVEIGRRLARECPVEADLVIGVPESGTPAAVGYAQESGVPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIRGKRLIVVDDSIVRGNTQRALVRMLREAGAVELHVRIASPPVKWPCFYGIDFPSPAELIANAVENEDEMLEAVRHAIGADTLGYISLRGMVAASEQPTSRLCTACFDGKYPIELPRETALGKNVIEHMLANAARGAALGELAADDEVPVGR
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
To generate a recombinant Mycobacterium tuberculosis Amidophosphoribosyltransferase (purF) protein in the E. coli expression system, the full-length mature purF gene fragment (35-527aa) specific to Mycobacterium tuberculosis along with an N-terminal 6xHis-tag is cloned into a suitable expression vector. The resulting recombinant plasmid is then transformed into E. coli host cells using a transformation method. The transformed cells are selected using antibiotic resistance markers to ensure the presence of the recombinant plasmid and are cultured in a growth medium optimized for protein expression. The cells are induced with an inducer molecule, such as IPTG, to initiate the expression of the recombinant purF protein. After incubation, the cells are harvested and lysed to release the intracellular contents including the expressed recombinant purF protein. The purity of the recombinant purF protein is assessed through SDS-PAGE analysis, demonstrating a purity level of up to 85%. On the gel, the recombinant Mycobacterium tuberculosis purF protein appears as a distinct band with a molecular weight of approximately 57 kDa.
