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Recombinant Mycobacterium tuberculosis Immunogenic protein MPT63 (mpt63)

Recombinant Mycobacterium tuberculosis Immunogenic protein MPT63 (mpt63)

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Code	CSB-EP358712GKB
Abbreviation	Recombinant Mycobacterium tuberculosis mpt63 protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP149074BA(A3) could indicate that this peptide derived from E.coli-expressed Mycobacterium tuberculosis mpt63. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP149074BA(A3) could indicate that this peptide derived from E.coli-expressed Mycobacterium tuberculosis mpt63.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

mpt63

Uniprot No.

P9WIP0

Research Area

Others

Alternative Names

mpt63; MT1977; Immunogenic protein MPT63; 16 kDa immunoprotective extracellular protein; Antigen MPT63

Species

Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

Source

E.coli

Expression Region

30-159aa

Target Protein Sequence

AYPITGKLGSELTMTDTVGQVVLGWKVSDLKSSTAVIPGYPVAGQVWEATATVNAIRGSVTPAVSQFNARTADGINYRVLWQAAGPDTISGATIPQGEQSTGKIYFDVTGPSPTIVAMNNGMEDLLIWEP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

17.7kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

In e.coli cells, the generation of recombinant Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) mpt63 protein involves cloning a DNA fragment encoding the Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) mpt63 protein (30-159aa) into a plasmid vector, which is then transferred into the e.coli cells. Positive cells are selected, cultured, and induced to express the mpt63 protein. A N-terminal 6xHis tag is attached to the protein. Lysis of the cells allows for the harvest of the recombinant Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) mpt63 protein. The collected recombiant Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) mpt63 protein is subjected to affinity purification and is identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. Its purity is greater than 90%.