Recombinant Mycobacterium tuberculosis Large-conductance mechanosensitive channel(mscL),partial

Code CSB-EP358693MVZ1b1
Size $2466
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names mscL
Uniprot No. P9WJN5
Research Area Others
Species Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Source E.coli
Expression Region 91-151aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 11.5 kDa
Protein Length partial
Tag Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 13-23 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Channel that opens in response to stretch forces in the membrane lipid bilayer. The force required to trigger channel opening depends on the nature of the membrane lipids; the presence of phosphatidylinositol enhances mechanosensitivity of the channel. May participate in the regulation of osmotic pressure changes within the cell.
Gene References into Functions
  1. Results show the interaction of lipids with MscL using atomistic molecular dynamics simulations and identify the simple motif that confers MscL with strong anchoring to the bilayer. PMID: 25437007
  2. residues in the primary gate in the first transmembrane domain of MscL are critical for mechanosensitive channel gating PMID: 15111403
  3. Data show that solubilization of membrane proteins such as the MscL by covalent attachment of amphiphiles results in homogeneous particles that may prove useful for crystallization, solution NMR spectroscopy, and electron microscopy. PMID: 15465059
  4. very efficient hydrophobic matching between the protein and the surrounding lipid bilayer PMID: 15823029
  5. binding site of high affinity for anionic phospholipids PMID: 15823046

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Subcellular Location Cell membrane; Multi-pass membrane protein.
Protein Families MscL family
Database Links

KEGG: mtu:Rv0985c

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