Recombinant Mycobacterium tuberculosis Methionine aminopeptidase 1 (map-1)

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Code CSB-EP765906MVZ
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Size US$388
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  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP765906MVZ could indicate that this peptide derived from E.coli-expressed Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) map-1.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP765906MVZ could indicate that this peptide derived from E.coli-expressed Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) map-1.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
map-1
Uniprot No.
Research Area
Signal Transduction
Alternative Names
map-1; mapA; MT0758Methionine aminopeptidase 1; MAP 1; MetAP 1; EC 3.4.11.18; Peptidase M
Species
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Source
E.coli
Expression Region
1-266aa
Target Protein Sequence
MRPLARLRGRRVVPQRSAGELDAMAAAGAVVAAALRAIRAAAAPGTSSLSLDEIAESVIRESGATPSFLGYHGYPASICASINDRVVHGIPSTAEVLAPGDLVSIDCGAVLDGWHGDAAITFGVGALSDADEALSEATRESLQAGIAAMVVGNRLTDVAHAIETGTRAAELRYGRSFGIVAGYGGHGIGRQMHMDPFLPNEGAPGRGPLLAAGSVLAIEPMLTLGTTKTVVLDDKWTVTTADGSRAAHWEHTVAVTDDGPRILTLG
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
32.9 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Our Recombinant Mycobacterium tuberculosis map-1 is a carefully crafted research tool that precisely mimics the native Methionine aminopeptidase 1 (MetAP 1; Peptidase M) protein found in the Mycobacterium tuberculosis strain CDC 1551 / Oshkosh. This protein is known for its role in the area of signal transduction, making it a valuable resource for related research endeavors.

This recombinant protein, produced in E.coli, offers a full-length representation, encompassing the 1-266aa expression region of the Methionine aminopeptidase 1. Featuring an N-terminal 10xHis-tag, the protein allows for enhanced detection and purification during experimental procedures. The purity of the Recombinant Mycobacterium tuberculosis map-1 is above 90%, as determined by SDS-PAGE, affirming its quality and suitability for your studies. Choose between liquid or lyophilized powder forms based on your research needs and storage facilities.

Customer Reviews and Q&A

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Target Background

Function
Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.
Protein Families
Peptidase M24A family, Methionine aminopeptidase type 1 subfamily
Database Links

KEGG: mtc:MT0758

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