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Recombinant Mycoplasma pneumoniae Uncharacterized protein MG218.1 homolog (MPN_311)

Recombinant Mycoplasma pneumoniae Uncharacterized protein MG218.1 homolog (MPN_311)

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Code	CSB-EP305096MLW
Abbreviation	Recombinant Mycoplasma pneumoniae Uncharacterized protein MG218.1 homolog
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

MPN_311

Uniprot No.

P75470

Research Area

Others

Species

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Source

E.coli

Expression Region

1-357aa

Target Protein Sequence

MTNDYQQLNYLVETDDEADIIIANLVKQLNELKEILLSLDNQDLEINQVNHRTTVHNTSSNTSNNSTSNNIADGNYNSNFFHNFSKETLQTQAKRGFLLLERCSLVGLQQLELEYLNVLGRSFESHQDKINFLVNLRDLTNDHLLDTEKIVSTLEQIFNVIGGTEYTPILNSFFNQSLNDPDPIQREIGLKQFVANLRQRFKTLLQKTNNSIQQLEAEIQIPTTHIKSDEVMFGPPDMNERLVLNDSETDAILRSIEAELESALQNKKQVVVTAVPPIAANLATDSISQETLEHNLNNTETVNTTTVTVTSASETQVRKPQISLRPIFQGNFPKRLSREDIQRYAHQLQELEQSNEG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

48.0 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Mycoplasma pneumoniae (strain ATCC 29342 / M129) MPN_311 protein expression involves transfecting e.coli cells with a DNA expression vector containing the gene encoding the recombinant protein of interest (1-357aa) and then culturing the cells so that they express the desired protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant Mycoplasma pneumoniae (strain ATCC 29342 / M129) MPN_311 protein is collected and purified from the cell lysate through affinity purification. Its purity is greater than 85%, as measured by SDS-PAGE.