Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

porA

Uniprot No.

P0DH58

Research Area

Others

Alternative Names

porA; NMB1429; Major outer membrane protein P.IA; PIA; Protein IA; Class 1 protein

Species

Neisseria meningitidis serogroup B (strain MC58)

Source

E.coli

Expression Region

20-392aa

Target Protein Sequence

DVSLYGEIKAGVEGRNYQLQLTEAQAANGGASGQVKVTKVTKAKSRIRTKISDFGSFIGFKGSEDLGDGLKAVWQLEQDVSVAGGGATQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMPVSVRYDSPEFSGFSGSVQFVPIQNSKSAYTPAYYTKNTNNNLTLVPAVVGKPGSDVYYAGLNYKNGGFAGNYAFKYARHANVGRNAFELFLIGSGSDQAKGTDPLKNHQVHRLTGGYEEGGLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

44.1 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 20-392 constitute the expression domain of recombinant Neisseria meningitidis serogroup B porA. The expected molecular weight for the porA protein is calculated to be 44.1 kDa. Expression of this porA protein is conducted in e.coli. The N-terminal 6xHis tag was smoothly integrated into the coding gene of porA, which enables a simple process of detecting and purifying the porA recombinant protein in the following steps.

The Neisseria meningitidis serogroup B major outer membrane protein P.IA (PorA) is a crucial antigenic protein that plays a significant role in the pathogenesis and immune response against Neisseria meningitidis, a bacterium responsible for causing meningococcal diseases. PorA is a porin protein known for its high variability, making it a key factor in distinguishing different strains. PorA is an important component of outer membrane vesicle (OMV) vaccines developed to protect against meningococcal B infections. Investigations into PorA contribute to the development of strategies to combat Neisseria meningitidis infections and enhance vaccine design, emphasizing the importance of understanding the antigenic properties of this major outer membrane protein.

Target Background

Function

Serves as a slightly cation selective porin. Major antigen on the gonococcal cell surface and it may have pathogenic properties in addition to its porin activity.

Subcellular Location

Cell outer membrane; Multi-pass membrane protein.

Protein Families

Gram-negative porin family

Database Links

KEGG: nme:NMB1429

STRING: 122586.NMB1429

Code	CSB-EP320687NGG
Abbreviation	Recombinant Neisseria meningitidis serogroup B porA protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Neisseria meningitidis serogroup B Major outer membrane protein P.IA (porA)

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