Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
porA; NMB1429; Major outer membrane protein P.IA; PIA; Protein IA; Class 1 protein
Species
Neisseria meningitidis serogroup B (strain MC58)
Expression Region
20-392aa
Target Protein Sequence
DVSLYGEIKAGVEGRNYQLQLTEAQAANGGASGQVKVTKVTKAKSRIRTKISDFGSFIGFKGSEDLGDGLKAVWQLEQDVSVAGGGATQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMPVSVRYDSPEFSGFSGSVQFVPIQNSKSAYTPAYYTKNTNNNLTLVPAVVGKPGSDVYYAGLNYKNGGFAGNYAFKYARHANVGRNAFELFLIGSGSDQAKGTDPLKNHQVHRLTGGYEEGGLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 20-392 constitute the expression domain of recombinant Neisseria meningitidis serogroup B porA. The expected molecular weight for the porA protein is calculated to be 44.1 kDa. Expression of this porA protein is conducted in e.coli. The N-terminal 6xHis tag was smoothly integrated into the coding gene of porA, which enables a simple process of detecting and purifying the porA recombinant protein in the following steps.
The Neisseria meningitidis serogroup B major outer membrane protein P.IA (PorA) is a crucial antigenic protein that plays a significant role in the pathogenesis and immune response against Neisseria meningitidis, a bacterium responsible for causing meningococcal diseases. PorA is a porin protein known for its high variability, making it a key factor in distinguishing different strains. PorA is an important component of outer membrane vesicle (OMV) vaccines developed to protect against meningococcal B infections. Investigations into PorA contribute to the development of strategies to combat Neisseria meningitidis infections and enhance vaccine design, emphasizing the importance of understanding the antigenic properties of this major outer membrane protein.
