Recombinant Pig POU domain, class 5, transcription factor 1 (POU5F1)

Code CSB-YP879986PI
MSDS
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Source Yeast
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Code CSB-EP879986PI
MSDS
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Source E.coli
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Code CSB-EP879986PI-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP879986PI
MSDS
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Source Baculovirus
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Code CSB-MP879986PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
POU5F1
Uniprot No.
Alternative Names
POU5F1; OCT3; OCT4; POU domain; class 5; transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3
Species
Sus scrofa (Pig)
Expression Region
1-360
Target Protein Sequence
MAGHLASDFA FSPPPGGGGD GPGGPEPGWV DPRTWLSFQG PPGGSGIGPG VGPGAEVWGL PACPPPYDFC GGMAYCAPQV GVGLVPQGGL ETPQPEGEAG AGVESNSEGA SPEPCAAPAG AAKLDKEKLE PNPEESQDIK ALQKDLEQFA KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGNLESMFL QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYSQREDFEA AGSPFPGGPV SFPLAPGPHF GTPGYGGPHF TTLYSSVPFP EGEAFPSVSV TPLGSPMHSN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Gene References into Functions
  1. Activin A and overexpression of SMAD2/3 significantly promoted expressions of porcine NANOG and OCT4,maintaining induced pluripotent stem cell self-renewal through up-regulation of Nanog/OCT4 expression. PMID: 27996082
  2. Our results indicate that continuous expression of OCT-4 in blastomeres is essential for trophectoderm formation of porcine embryos. PMID: 27210587
  3. These findings suggest that the 12-bp indel polymorphism of the Oct4 gene might be a potential DNA marker for selecting preferred individuals in relation to reproductive traits in pig marker-assisted selection breeding. PMID: 28139300
  4. Oct4-overexpression enhances porcine ovarian stem cell differentiation in to oocyte-like cells. PMID: 27067537
  5. showed novel molecular regulation of CDX2 on Oct4, and provided important clues for clarifying the mechanism of interaction between CDX2 and Oct4 in embryo of mammals other than mouse PMID: 26708097
  6. Overexpression of Sox2 or Oct4 in bone mesenchymal stem cells in culture media containing a basic fibroblast growth factor results in higher proliferation and differentiation compared to controls. PMID: 24338401
  7. Oct4 positive stem/progenitor swine lung epithelial cells are targets for influenza virus replication. PMID: 22491467
  8. study shows that localization of octamer-binding protein 4(OCT4) is associated with an embryonic stem cell (ESC)-like morphology from porcine inner cell mass PMID: 20663092
  9. OCT4 expression, in contrast to earlier speculations, at least in hatched blastocysts, resembles the expression pattern in the mouse embryo. PMID: 21089074
  10. cells isolated from umbilical cord express three transcription factors,Oct-4, Sox-2 & Nanog, found in pluripotent stem cell markers both at the mRNA and protein level. PMID: 16460563
  11. in the forming endoderm at the primitive streak stage, OCT4 was detectable in potential primordial germ cells only; in the ectoderm and mesodermal cell lineages OCT4 was cleared at the neural groove and somite stage, respectively PMID: 16541449

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
POU transcription factor family, Class-5 subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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